An Evaluation of Signal Amplification by the Ion Channel Sebsir Based on a Glutamate Receptor Ion Channel Protein

Minami, Hirotsugu; Uto, Masayuki; Sugawara, Masao; Odashima, Kazunori; Umezawa, Yoshio; Michaelis, Elias K.; Kuwana, Theodore

doi:10.2116/analsci.7.supple_1675

Cited by 16 publications

(5 citation statements)

References 6 publications

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“…Sensors based on the glutamate ion channel receptor (GluR) were developed for the detection of l -glutamate and other agonists presented in nanomolar concentration. − GluR isolated from rat synaptic plasma membranes was incorporated into planar BLMs. The integrated multichannel current, corresponding to the sum of the total amount of ions that had passed through the multiple open channels, was used as a measure of agonist affinity and concentration.…”

Section: Receptors and Their Classificationmentioning

confidence: 99%

Application of Natural Receptors in Sensors and Assays

2002

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Biosensors are analytical devices that use a biological or biologically derived material immobilized at a physicochemical transducer to measure one or more analytes. Although there are a large number of reviews on biosensors in general, there has been little systematic information presented on the application of natural receptors in sensor technology. This perspective discusses broadly the fundamental properties of natural receptors, which make them an attractive option for use as biorecognition elements in sensor technology. It analyses the current situation by reference to typical examples, such as the application of nicotinic acetylcholine receptor and G protein-linked receptors in affinity sensors and analyses the problems that need to be resolved prior to any commercialization of such devices.

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Section: Receptors and Their Classificationmentioning

confidence: 99%

Application of Natural Receptors in Sensors and Assays

2002

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“…6 To gain molecular recognition, receptors must be incorporated in a reconstituted biomembrane able to mimic the natural environment in which the selective interaction with substrate produces a membrane disruption that can be electrochemically trasducted, 1,2,7-12 as in the case of ion-channel-linked receptors, where the specific ligand-receptor interaction transiently opens or closes the ion gate, thus changing the membrane ion permeability. 13 In the last years, various natural receptors, including glutamate ionotropic receptor, [14][15][16][17][18][19] nicotinic acetylcholine receptor ion- † Dipartimento di Chimica, Universita `di Roma "La Sapienza". ‡ Dipartimento di Scienze Biochimiche "A. Rossi Fanelli", Universita di Roma "La Sapienza".…”

Section: Introductionmentioning

confidence: 99%

“…In the last years, various natural receptors, including glutamate ionotropic receptor, − nicotinic acetylcholine receptor ion- channel, , Na + / d -glucose cotransporter, lactose/H + cotransporter, , Na + ,K + -ATPase, and DNA, have been tested as sensing elements. Semiartificial receptors were also obtained by modifying well-known peptides such as Val 26 and GramD [widely used for testing reconstructed bilayer lipid membranes (BLMs)] achieving biosensors for detecting DNA or antibodies. , Receptor-based biosensors were also obtained by embedding fully artificial receptors into BLMs …”

Section: Introductionmentioning

confidence: 99%

Glutamate Receptor Incorporated in a Mixed Hybrid Bilayer Lipid Membrane Array, as a Sensing Element of a Biosensor Working under Flowing Conditions

et al. 2005

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The realization of a reliable receptor biosensor requires stable, long-lasting, reconstituted biomembranes able to supply a suitable biomimetic environment where the receptor can properly work after incorporation. To this end, we developed a new method for preparing stable biological membranes that couple the biomimetic properties of BLMs (bilayer lipid membranes) with the high stability of HBMs (hybrid bilayer membranes); this gives rise to an innovative assembly, named MHBLM (mixed hybrid bilayer lipid membrane). The present work deals with the characterization of biosensors achieved by embedding an ionotropic glutamate receptor (GluR) on MHBLM. Thanks to signal (transmembrane current) amplification, which is typical of natural receptors, the biosensor here produced detects glutamate at a level of nmol L(-1). The transmembrane current changes linearly vs glutamate up to 100 nmol L(-1), while the limit of detection is 1 nmol L(-1). In addition, the biosensor response can be modulated both by receptor agonists (glycine) and antagonists (Mg(2+)) as well, and by exploiting the biosensor response, the distribution of different kinds of ionotropic GluR present in the purified sample, and embedded in MHBLM, was also evaluated. Finally, one of the most important aspects of this investigation is represented by the high stability of the biomimetic system, which allows the use of biosensor under flowing conditions, where the solutions flow on both biomembrane faces.

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“…[7][8][9][10][11] A membrane-protein-based ion-channel sensor for glutamate and active (uphill) transport sensor for glucose were first reported later in 1990 and 1993, respectively. [12][13][14][15] Coulometric biosensors using glutamate receptor (GluR) ion channel protein as a signal-amplifying sensory element that exploits the glutamate-triggered Na + ion current through bilayer lipid membranes have been fabricated. 13 The formation of stable planar bilayer lipid membranes was achieved by applying the folding method across a small circular aperture bored through a thin polyimide film.…”

Section: Ion-channel Sensors and Active Transport Sensorsmentioning

confidence: 99%