An evaluation of sampling effects on multiple DNA barcoding methods leads to an integrative approach for delimiting species: A case study of the North American tarantula genus Aphonopelma (Araneae, Mygalomorphae, Theraphosidae)

The Dynamic Discipline of Species Delimitation: Progress Toward Effectively Recognizing Species Boundaries in Natural Populations

“…Furthermore, research suggests that the so-called single-threshold version of the GMYC method likely outperforms the multiple-threshold approach (Fujisawa and Barraclough 2013;Monaghan et al 2009). However, other studies suggest that the GMYC method may often provide higher estimates for the total number of OTUs than other molecular species delimitation methods (Hamilton et al 2014;Miralles and Vences 2013;Talavera et al 2013), warranting a cautious interpretation of results from GMYC analyses.…”

“…However, an ongoing appeal to researchers to assess species boundaries from multiple and complementary perspectives (phylogenetics, population genetics, comparative morphology, development, ecology, etc. ) has resulted in an integrative taxonomic framework bringing these conceptual and methodological perspectives together (Hamilton et al 2014;Fujita et al 2012;Salicini et al 2011;Yeates et al 2011;Padial et al 2010;Dayrat 2005;Will et al 2005;Wiens and Penkrot 2002). In practice, any study linking different kinds of data to support hypotheses of species boundaries, including mapping morphological characters onto a molecular phylogeny, can be considered integrative.…”

Comparison of five methods for delimitating species in Ophion Fabricius, a diverse genus of parasitoid wasps (Hymenoptera, Ichneumonidae)

“…The jMOTU analysis used thresholds of 7, 13 and 20 base pairs, or approximately 1%, 2%, and 3% sequence divergence. (Bergsten et al, 2012;Hamilton et al, 2014). In theory, even (Ceccarelli et al, 2012;Lim et al, 2012;Monagahan et al, 2009).…”

Multilocus sequence data reveal dozens of putative cryptic species in a radiation of endemic Californian mygalomorph spiders (Araneae, Mygalomorphae, Nemesiidae)

“…3). 403 Using the 5% threshold (similar to Hamilton et al, 2014), the num-404 ber of species identified more than doubles to 59 species (Fig. 3, 405 Appendix).…”

“…An examination of female reproduc- Mygalomorph spiders, the radiation that includes tarantulas 48 and trapdoors spiders, are ancient and diverse. Because of general 49 dispersal limitation and high levels of regional endemism, several 50 research groups from different continents have developed mygalo-51 morphs as models for studies of species delimitation, cryptic spe-52 ciation, and historical biogeography (e.g., Bond and Stockman,53 2008; Satler et al, 2013;Hedin et al, 2013;Hendrixson et al, 54 2013; Castalanelli et al, 2014;Hamilton et al, 2014;Opatova 55 and Arnedo, 2014a). Still, many features of mygalomorph biology 56 make them challenging to study.…”