An evaluation of sampling- and culturing methods in the Norwegian action plan against Campylobacter in broilers

Sandberg, Marianne; Østensvik, Øyvin; Aunsmo, Agnete Lien; Skjerve, Eystein; Hofshagen, Merete

doi:10.1016/j.ijfoodmicro.2005.09.007

Cited by 12 publications

(10 citation statements)

References 3 publications

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“…The collection of faecal samples was based on the method described by Sandberg et al., 2006. In each sector, ten individual fresh faecal droppings, each consisting of approximately 5 g of faecal material, were collected and mixed in a sterile pot using a wooden spatula.…”

Section: Methodsmentioning

confidence: 99%

“…Several sampling methods are in use to detect Campylobacter in broiler houses, including cloacal swabs (Hansson et al., 2004), faecal samples (Sandberg et al., 2006), caecal contents (Allen et al., 2007; Rosenquist et al., 2009) and boot swabs or the equivalent boot sock model (Bull et al., 2006; Ellis‐Iversen et al., 2011; Ridley et al., 2011). Boot swabs are a widely used sampling method for Salmonella testing in broilers, turkeys and laying hens on floor systems and for the statutory monitoring of chicken breeding flocks (Mueller‐Doblies et al., 2009).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of C. jejuni and C. coli from Broiler Flocks at Primary Production

Vidal

Rodgers

Arnold

et al. 2012

Zoonoses and Public Health

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The objective of the study was to evaluate the performance of different combinations of sample type, transport medium and culture methods for the recovery of Campylobacter jejuni and C. coli from broiler flocks at primary production. Boot swabs moistened with one of four different transport media [maximum recovery diluent (n=120), Exeter broth (EX) (n=120), buffered peptone water (n=120) and modified semi-solid Cary-Blair (n=120)], caecal samples (n=40) and faecal samples (n=120) from 40 broiler flocks were compared and sensitivity estimates obtained using a Bayesian model. Samples were cultured onto mCCDA before and after enrichment in EX and incubated microaerobically at 41.5°C. Campylobacter suspect colonies were identified to the species level by multiplex PCR. Results from the Bayesian model indicated that boot swabs after enrichment had higher sensitivity (90-94%) than caecal contents before or after enrichment (84% and 89%, respectively) and faecal samples after enrichment (82%) for the detection of Campylobacter spp., although these differences were not statistically significant. Enrichment significantly increased the sensitivity of boot swab and caecal samples for detection of Campylobacter spp. and C. jejuni, respectively. However, the enrichment of caecal samples resulted in a significant decrease in the sensitivity of these samples for detection of C. coli. There was much greater variation in the sensitivity estimates of the methods for detecting C. coli than for C. jejuni, and the ranking of methods was different between the two species. Boot swabs gave the best sensitivity values for detection of C. jejuni, and enrichment culture of faecal samples was the most sensitive method for detection of C. coli.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of C. jejuni and C. coli from Broiler Flocks at Primary Production

Vidal

Rodgers

Arnold

et al. 2012

Zoonoses and Public Health

View full text Add to dashboard Cite

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“…On the other hand, while Revez et al (2008) evaluate caecal contents, Piccirillo et al (2011) examined rectal swabs. Several sampling methods are in use to detect Campylobacter in broiler houses, including cloacal swabs (Hansson et al, 2004), faecal samples (Sandberg et al, 2006), caecal contents (Allen et al, 2007;Rosenquist et al, 2009). However, there is not yet an accepted standard method for the detection and isolation of Campylobacter spp.…”

Section: Resultsmentioning

confidence: 99%

Short communication: Absence of campylobacter spp. In intensive rabbit farming in eastern Spain, preliminary results

2016

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Campylobacteriosis and salmonellosis are the most frequently reported zoonoses and among the most common causes of diarrhoeal illness in the European Union and the United States, and their incidence appears to be increasing. Campylobacter species are routinely found in poultry, swine, cattle, dairy cows and sheep. So far, there are few descriptions of Campylobacter isolation from rabbits. Rabbit meat is a common item in the Mediterranean diet. In this context, the aim of the present study was to investigate the occurrence of Campylobacter spp. in healthy rabbits reared in intensive farms in the Alto Palancia region, eastern Spain. Caecal contents from 70 healthy does reared on 7 different farms were collected. Bacteriological culture was performed in accordance with ISO 10272-1:2006. All samples tested negative for Campylobacter spp. To our knowledge, this is the first study in which comprehensive monitoring was specifically carried out in order to provide data on the occurrence of thermophilic Campylobacter spp. in large intensive rabbit farms in Spain. However, further microbiological studies throughout the Spanish territory are needed to determine the prevalence and risk of other foodborne pathogens in rabbits at farm level.

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“…From the AFLP profiles, the 20-25 observed bands ranging from 50 to 450 bp in size, were included in the analysis. The discriminatory power of the method was verified by analysing 24 strains from eight different flocks isolated in a previous project (Sandberg et al 2005b), results not shown. The relatedness between the strains was analysed with bionumerics 3.0 software (Applied Maths, Kortrijk, Belgium).…”

Section: Discussionmentioning

confidence: 96%

Genetic variability of Campylobacter jejuni isolated from fresh and frozen broiler carcasses

et al. 2006

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Aims: The aim of this study was to determine the genetic variability of Campylobacter jejuni isolates from poultry before and after freezing treatment in order to identify genotypes that would survive the treatment. Methods and Results: C. jejuni was isolated from both fresh and frozen halves of the same carcass after freezing for 2 or more than 20 days at −20°C. From 36 carcasses, representing five unrelated flocks in Norway, a total of 209 isolates were included in the study. Thirty‐two of the isolates were recovered with a qualitative method while the remaining 177 were isolated using a quantitative method. Isolates were genotyped with fluorescent amplified fragment length polymorphism using MfeI and BglII restriction enzymes. Nine different genotypes were identified, however, one genotype was shown to be dominant in three different flocks. This genotype and the dominant genotype of another flock were found among isolates from fresh and frozen broiler halves. They were also shown to be identical to genotypes frequently identified among strains isolated from humans, cattle and poultry flocks in previous years. Conclusions: Freezing treatment or isolation method appeared not to select for a particular genotype. Significance and Impact of the Study: The results of the present study indicate that the freezing tolerance of strains is not genotype dependent.

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An evaluation of sampling- and culturing methods in the Norwegian action plan against Campylobacter in broilers

Cited by 12 publications

References 3 publications

Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of C. jejuni and C. coli from Broiler Flocks at Primary Production

Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of C. jejuni and C. coli from Broiler Flocks at Primary Production

Short communication: Absence of campylobacter spp. In intensive rabbit farming in eastern Spain, preliminary results

Genetic variability of Campylobacter jejuni isolated from fresh and frozen broiler carcasses

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