An evaluation of methods to decrease the availability of inorganic sulphate for sulphate conjugation in the rat in vivo

Krijgsheld, Klaas R.; Scholtens, Egbert; Mulder, Gerard J.

doi:10.1016/0006-2952(81)90208-2

Cited by 45 publications

(20 citation statements)

References 25 publications

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“…The serum sulfate level in humans has been shown to increase dramatically 3-8 hours after ingestion of a single large amount of protein (12); in rats, sulfate levels have been shown to drop 30-50% after an overnight fast (11). Feeding rats a diet deficient in protein for 4 days has been shown to reduce sulfate levels by 50% (18).…”

Section: Discussionmentioning

confidence: 99%

“…It is noteworthy that several drugs, including aspirin and acetaminophen, are metabolized for excretion by conjugation with sulfate. Use of these agents has been shown to lower sulfate levels in humans (13,(24)(25)(26) and in other animals as well (18,(27)(28)(29). For these reasons, it remains possible that sulfate levels in some people under specific circumstances of protein consumption and/or drug ingestion could affect OA, even though results of the current study have shown no such effect.…”

Section: Discussionmentioning

confidence: 99%

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Fasting serum sulfate levels before and after development of osteoarthritis in participants of the veterans administration normative aging longitudinal study do not differ from levels in participants in whom osteoarthritis did not develop

Blinn¹,

Dibbs²,

Hronowski³

et al. 2005

Arthritis & Rheumatism

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Objective. To determine whether the development of osteoarthritis (OA) in men over a 33-year period is related to lower sulfate levels in stored serum collected during that time interval.Methods. Stored serum samples from participants in the Veterans Administration Normative Aging Study were assayed for sulfate by ion-exchange chromatography. Samples had been obtained every 3-5 years during part or all of a 33-year portion of the study. Sulfate levels were determined in serum from all participants who underwent knee replacement surgery and had evidence of radiographic hand OA, from some of the participants who had evidence of radiographic hand OA but had not undergone knee replacement surgery, from all participants who underwent knee replacement surgery but had no evidence of radiographic hand OA, and from age-matched participants who had no evidence of OA by history, physical examination, or hand radiography.Results. Serum sulfate levels in participants, with or without radiographic hand OA and/or knee replacements, who were ages 34-72 years at the first examination, ranged from 0.21 mM to 0.51 mM over the course of a maximum of 33 years. Both the overall mean and median sulfate levels rose from 0.32 mM at age 40-50 years to 0.38 mM at age 70-80 years, and the overall mean and median for all ages was 0.36 mM. There were no significant differences in sulfate levels between subjects in any of the 4 groups.Conclusion. There was no evidence of a relationship between these serum sulfate levels and the development of OA. However, all samples were collected after overnight fasting, and no participant was younger than age 34 years at the initiation of the study. It remains to be determined whether differences in the time of ingestion of daily dietary protein providing sulfate are related to the development of OA, or whether sulfate levels measured at an earlier age could be a factor.Proteochondroitin sulfate, primarily aggrecan, plays a major role in the mechanical support of cartilage. In addition to assisting in the positioning and orientation of collagen, it determines salt and water distribution and controls a volume domain of water many times the volume of the proteoglycan itself. This provides a major degree of cushioning, so that water is expressed from the domain under pressure and then can return when the pressure is released. These functions are dependant on the high charge of the sulfate substituents; therefore, any decrease in sulfation might be expected to affect the structure and stability of the cartilage. In addition, undersulfation might increase susceptibility to animal chondroitin-degrading enzymes, because these enzymes degrade much more readily whenever sulfate is absent from the adjacent sugars of chondroitin (1,2). Consequently, undersulfation could produce a functional in-

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fasting serum sulfate levels before and after development of osteoarthritis in participants of the veterans administration normative aging longitudinal study do not differ from levels in participants in whom osteoarthritis did not develop

Blinn¹,

Dibbs²,

Hronowski³

et al. 2005

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…Sulphate levels in rats have been shown to drop by 30-50% after an overnight fast, 4 and rats fed a diet deficient in protein for 4 days have been shown to have 50% decrease in sulphate levels. 11 Although serum levels of sulphate in normal humans have been shown to increase dramatically from 3 to 8 h after ingestion of a single large amount of protein, 5 no studies of sulphate levels in humans during fasting or in the absence of dietary protein have been reported. Diurnal variations in serum or plasma sulphate in animals 4 and humans 6 have been described, but these studies do not seem to have been carried out under fasting conditions.…”

mentioning

confidence: 99%

Sulphate and osteoarthritis: decrease of serum sulphate levels by an additional 3-h fast and a 3-h glucose tolerance test after an overnight fast

Blinn

Biggee

McAlindon

et al. 2006

Annals of the Rheumatic Diseases

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“…Inorganic sulfate was measured by a turbidimetric method that has been described and modified by Krijgsheld et al (16,17). To 500 PI of (diluted) serum or urine containing 0.1-1.3 mM inorganic sulfate, 2 ml of trichloroacetic acid solution (5% weight/volume) was added, and the mixture was allowed to stand at room temperature for 10 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…Salicylate standards were established using normal mouse serum obtained from agematched mice. By using microplates (instead of cuvettes) and a Titertek Multiscan (Flow Laboratories, Irvine, Scotland) to read the absorbance, 20 pl of serum was sufficient for a duplicate determination (reproducible within a range of 2 10%).Inorganic sulfate was measured by a turbidimetric method that has been described and modified by Krijgsheld et al(16,17). To 500 PI of (diluted) serum or urine containing 0.1-1.3 mM inorganic sulfate, 2 ml of trichloroacetic acid solution (5% weight/volume) was added, and the mixture was allowed to stand at room temperature for 10 minutes.…”

mentioning

confidence: 99%

Salicylate‐induced depletion of endogenous inorganic sulfate. Potential role in the suppression of sulfated glycosaminoglycan synthesis in murine articular cartilage

Vries¹,

Berg²,

Putte³

1985

Arthritis & Rheumatism

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Sodium salicylate has been shown to suppress glycosaminoglycan (GAG) synthesis by articular hyaline cartilage in vitro. We investigated the in vivo effect of sodium salicylate on murine patellar cartilage, using incorporation of intraperitoneally administered 35S-sulfate as a measure of sulfated GAG synthesis. Our results indicated that a single dose of sodium salicylate (200 mg/ kg) inhibited in vivo sulfated GAG synthesis by 56%, compared with controls, and had no effect on sulfated GAG breakdown. A striking finding was that sodium sulfate treatment reduced the serum concentration of inorganic sulfate from 1.1 mM to approximately 0.3 mM, and that this serum reduction was associated with a twofold increase in urinary excretion of sulfate. Using anatomically intact patellar cartilage, in vitro studies clearly showed that, in concentrations reached in vivo (21 mM), salicylate suppressed murine chondrocyte GAG synthesis. However, in the presence of serum, the effects of 1 mM salicylate were abolished. We also found that sulfated GAG synthesis was clearly inhibited at low concentrations of sulfate (<0.5 mM). Our data indicate that sodium salicylate can suppress articular chondrocyte sulfated GAG synthesis in vivo, and that this effect may particularly be due to a drug-induced reduction of endogenous sulfate. Several nonsteroidal antiinflammatory drugs (NSAIDs) commonly used in the treatment of arthritis have been shown to provoke suppression of glycosaminoglycan (GAG) synthesis in organ cultures of normal articular cartilage (1-3). Studies have clearly shown that salicylate administration suppresses in vitro GAG synthesis in normal human articular cartilage and that degenerated cartilage is affected even more by this drug (43). In experiments on canine osteoarthritic cartilage (6), an almost complete suppression was observed at salicylate concentrations of 10-3M (14 mg%), a level which is readily achieved in human serum. ~~Because patients with rheumatic diseases often receive long-term treatment with NSAIDs, it is relevant to know whether the suppressive effect of these drugs, as observed in vitro, also occurs in vivo. A deleterious effect of salicylates on articular cartilage after long-term treatment has been shown in studies of animals (7,8). However, the chondrocyte synthetic function of salicylate-treated animals has only been tested ex vivo (7,9), and a direct action of salicylates on in vivo articular GAG synthesis has not been established.Originally, the present study was aimed at investigations of the short-term effect of sodium salicylate on in vivo sulfated GAG synthesis in normal articular cartilage. A preliminary experiment showed that sodium salicylate indeed inhibited 35S-sulfate incorporation in articular cartilage, but the most striking observation was a decrease in the serum content of 35S-sulfate, which paralleled a decrease in the endogenous serum sulfate level (10). With these observations and those of others (ll), who found that the lowering of environmental inorganic sulfate per se may SALI...

show abstract

An evaluation of methods to decrease the availability of inorganic sulphate for sulphate conjugation in the rat in vivo

Cited by 45 publications

References 25 publications

Fasting serum sulfate levels before and after development of osteoarthritis in participants of the veterans administration normative aging longitudinal study do not differ from levels in participants in whom osteoarthritis did not develop

Fasting serum sulfate levels before and after development of osteoarthritis in participants of the veterans administration normative aging longitudinal study do not differ from levels in participants in whom osteoarthritis did not develop

Sulphate and osteoarthritis: decrease of serum sulphate levels by an additional 3-h fast and a 3-h glucose tolerance test after an overnight fast

Salicylate‐induced depletion of endogenous inorganic sulfate. Potential role in the suppression of sulfated glycosaminoglycan synthesis in murine articular cartilage

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