An evaluation of laboratory ensiling techniques

Wilson, Robert F.; Wilkins, R. J.

doi:10.1002/jsfa.2740230315

Cited by 54 publications

(38 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous research has indicated that the size and type of laboratory silos, as well as the amount of forage ensiled, have little effect on silage fermentation. Wilson and Wilkins (1972) compared silages made from 18 grasses and eight legumes, produced in PVC bags, test-tubes and polythene bag silos, containing respectively 1 t, 100 g and 6 kg of forage, and concluded that the fermentation profile in the three types of silos agreed closely. Rodrigues et al (2002) did not detect differences in major fermentation parameters (pH, N-NH 3 , volatile fatty acids, lactic acid) and DML when comparing corn silages produced in plastic buckets (12 L), plastic bags (40 L) and concrete pipes (630 L).…”

Section: Resultsmentioning

confidence: 98%

“…The usefulness of laboratory silos in forage conservation research has been long established (Wilson and Wilkins, 1972), but studies on the effects of differences in laboratory silos on silage fermentation are scarce (O'Kiely andWilson, 1991 in Cherney et al, 2004;Rodrigues et al, 2002;Cherney et al, 2004). Analysis of fermentation products is normally carried out in water extracts produced in a blender or stomacher and, less frequently, in silage juices produced by the use of a hydraulic press.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of sugarcane laboratory ensiling and analysis techniques

Pedroso

Júnior

et al. 2014

R. Bras. Zootec.

View full text Add to dashboard Cite

-The objective was to evaluate the effects of laboratory-silo type and method of silage extract production, respectively, on sugarcane silage fermentation and recovery of fermentation products. Sugarcane was mechanically harvested and ensiled in three different types of laboratory silos (five replicates): 9.7 × 30 cm PVC tubes with tight lids, equipped or unequipped with Bunsen valves, and 20 L plastic buckets with tight lids and Bunsen valves. Three methods were used to produce silage extracts for pH, ethanol, acetic and lactic acids determination: extraction of silage juice by a hydraulic press and production of water extracts using a stomacher or a blender. Total dry matter loss (231 g/kg DM) was not affected by silo type. No interactions between silo type and method of silage extract production were observed for ethanol and organic acids contents in the silages. Interaction between silo type and method of silage extract preparation was detected for pH. Silo type affected ethanol content but did not affect lactic and acetic acids concentration in the silages. Dry matter, crude protein, neutral detergent fiber and ash were not affected by silo type. The method used to produce silage extracts affected the recovery of all fermentation products analyzed in the silages. Recovery of ethanol and acetic acid was higher when silage extracts were produced using a blender. For lactic acid recovery, the hydraulic press method was superior to the other two methods. Silage fermentation pattern is not affected by silo type, but the method used to produce silage extracts and some characteristics of silos affect the recovery of volatile fermentation products.

show abstract

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Evaluation of sugarcane laboratory ensiling and analysis techniques

Pedroso

Júnior

et al. 2014

R. Bras. Zootec.

View full text Add to dashboard Cite

show abstract

“…Water-soluble carbohydrate was determined in freeze-dried samples by the alkaline-ferricyanide method of Hagedorn & Jensen (1923) adapted for use with a Technicon Autoanalyser. The micro-diffusion method of Conway (1957) was used for ammonia determination, VFA were determined by gas-liquid chromatography (Wilson & Wilkins, 1972) and lactic acid by eerie sulphate oxidation (Elsden & Gibson, 1954). Chromium and calcium were determined by atomic absorption spectroscopy, sodium and potassium by flame photometry and phosphorus by a colorimetric method using vanado-molybdate.…”

Section: Methodsmentioning

confidence: 99%

Nutritional studies with sheep fed conserved ryegrass. 1. Silage and dried grass offered ad libitum without supplements

Wernli

Wilkins

1980

J. Agric. Sci.

Self Cite

View full text Add to dashboard Cite

Silage, dried-grass wafers and dried-grass pellets all prepared from a single crop of perennial ryegrass were given ad libitum as sole diets to six rumen-fistulated wether sheep.Feed intake, intake of digestible nutrients and the concentration of volatile fatty acids (VTA) in the rumen were lowest with silage, highest with pellets and intermediate with wafers. Conversely, digestibility, eating and ruminating time per kg D.M. consumed, rumen pH, the rate of D.M. and cellulose digestion in the rumen and the mean retention time of digesta in the alimentary tract were highest for silage, intermediate for wafers and lowest for pellets. The pattern of rumen fermentation differed significantly between silage and the dried-grass treatments. The rumen fluid contained higher proportions of acetic, iso-butyric, n-valeric and iso-valeric acids, but lower proportions of propionic and n-butyric acids with silage than with dried grass. Differences in rumen "VFA between the two forms of dried grass were small. Nitrogen retention was lowest for the silage treatment and this was associated with a high concentration of ammonia in the rumen.Low nitrogen status with silage could have limited voluntary intake, whereas the difference in intake between the wafer and pellet treatments probably arose from differences in the retention time of digesta in the alimentary tract. INTRODUCTIONm t w o ^o r m s > P e U ets a n ( i wafers. Ruminal and metabolic measurements were made and related to It is well established that the intake of D.M. intake and digestibility, from unwilted silage is lower than that of either fresh or dried forage or hay of similar digestibility (Moore, Thomas & Sykes, I960; Harris & Raymond,

show abstract

“…The lactic acid content of the extract was determined by the ceric sulphate oxidation method,5 ammonia content by a micro-diffusion technique6 and volatile fatty acids (VFA) by g.l.c. 3 Samples of fresh forages were taken for pH, dry matter (DM), WSC and total nitrogen (TN) determinations. DM was determined by oven-drying for 16 h at lOO"C, WSC as for the silages and TN by a Technicon method involving alkaline phenate as colorimetric reagent.…”

Section: Methodsmentioning

confidence: 99%