An evaluation of indirect immunofluorescence in the serological diagnosis of Nosema cuniculi infection

Simple diagnostic test for antibodies to Encephalitozoon cuniculi based on enzyme immunoassay

“…Since the EIA test was performed with a specific anti-IgG conjugate, this result should not be treated as a false negative. The 1FT negative, EIA positive serum was from a rabbit in a colony free from E. cuniculi (Cox et al, 1977). However the ratio was 1•51 and duplicate retests both gave a negative result.…”

“…Included amongst the sera used in this evaluation were some from rabbits from a colony known to be free from encephalitozoonosis (Cox, Gallichio, Pye & Walden, 1977), sera from naturally-infected rabbits used in an earlier evaluation of the immunofluorescence test (Cox & Gallichio, 1977, 1978, sera from artificially-infected rabbits, (Cox, Hamilton & Attwood, 1979) and sera from Scottish wild rabbits (Cox & Ross, 1980).…”

“…Standard procedures for testing sera for antibodies to E. cuniculi have been described in detail elsewhere (Cox & Pye, 1975), and modified methods for the production of E. cuniculi spores in foetal human fibroblast cell culture were reported by Cox et al (1977)…”

Phacoemulsification for the management ofEncephalitozoon cuniculi‐induced phacoclastic uveitis in a rabbit

“…High IFAT titers of similar magnitude to the present case have been documented in laboratory rabbits infected with E. cuniculi. 38 Humoral response to E. cuniculi in rabbits is mounted shortly after infection and persists for many months, if not for the duration of the infection 39 …”

Microsporidia (Encephalitozoon cuniculi) in wild rabbits in Australia

“…16 The titres in the laboratory rabbits tested were up to 10,240 and those in the wild rabbits tested were up to 5120. Previous studies [17][18][19] have reported high IFAT titres (over 8000) in sera from laboratory rabbits infected with E cuniculi. We found no evidence of E cuniculi spores in the urine of seropositive wild rabbits, although spores were isolated from the brain and kidney of seropositive wild and laboratory rabbits.…”