2020
DOI: 10.1038/s41467-020-17317-y
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An evaluation of COVID-19 serological assays informs future diagnostics and exposure assessment

Abstract: The world is entering a new era of the COVID-19 pandemic in which there is an increasing call for reliable antibody testing. To support decision making on the deployment of serology for either population screening or diagnostics, we present a detailed comparison of serological COVID-19 assays. We show that among the selected assays there is a wide diversity in assay performance in different scenarios and when correlated to virus neutralizing antibodies. The Wantai ELISA detecting total immunoglobulins against … Show more

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Cited by 345 publications
(441 citation statements)
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References 9 publications
(14 reference statements)
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“…For the human samples, diagnostic RT-PCR was performed for the E and the RdRp gene (42). In addition, serology was performed, using the Wantai Ig total and IgM ELISA, following the manufacturer's instructions (43). For all samples with a Ct value <32, sequencing was performed using a SARS-CoV-2 specific multiplex PCR for Nanopore sequencing, as previously described (3).…”
Section: Sars-cov-2 Diagnostics and Sequencingmentioning
confidence: 99%
“…For the human samples, diagnostic RT-PCR was performed for the E and the RdRp gene (42). In addition, serology was performed, using the Wantai Ig total and IgM ELISA, following the manufacturer's instructions (43). For all samples with a Ct value <32, sequencing was performed using a SARS-CoV-2 specific multiplex PCR for Nanopore sequencing, as previously described (3).…”
Section: Sars-cov-2 Diagnostics and Sequencingmentioning
confidence: 99%
“…Neutralization of live SARS-CoV-2 was measured by PRNT at day 30 post-vaccination in both BALB/c and C57BL/6 mice. Similar protocols have been published previously for SARS-CoV-2 [48]. Briefly, mouse sera were serially diluted from 1:20 to 1:320 in culture media and incubated with the Singapore isolate of SARS-CoV-2 virus for 1 hr at 37C.…”
Section: Plaque Reduction Neutralization Titer (Prnt)mentioning
confidence: 99%
“…5,6 Serologic detection of patient derived antibodies can be used to track SARS-CoV-2 progression and immunity, but has limited early detection ability. [6][7][8] Direct detection of SARS-CoV-2 has been proposed in scalable, rapidly deployed formats, but often suffers from low sensitivity that limits effectivity in general population testing. 9,10 Thus, the discovery of additional reagents to enable early and rapid SARS-CoV-2 detection and/or neutralization is critical.…”
Section: Introductionmentioning
confidence: 99%