An evaluation of clinical performance of FTA cards for HPV 16/18 detection using cobas 4800 HPV Test compared to dry swab and liquid medium

Li, Dong; Lin, Chunqing; Li, Li; Wang, Margaret; Cui, Jianfeng; Feng, Ruimei; Liu, Bin; Wu, Zeni; Liu, Jia; Liao, Guang-Dong; Chen, Wen; Qiao, You‐Lin

doi:10.1016/j.jcv.2017.06.008

Cited by 9 publications

(12 citation statements)

References 28 publications

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“…evaluated FTA and liquid samples with Cobas in a small study and also found good concordance. Interestingly, they found the same concordance for dry brush samples which for cost and simplicity is an important future direction [21].…”

Section: Discussionmentioning

confidence: 63%

An evaluation of solid versus liquid transport media for high-risk HPV detection and cervical cancer screening on self-collected specimens

Duan

Yan

et al. 2020

Infect Agents Cancer

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Background The solid transport media is a small size card that allows fast, easy DNA extraction from a variety of biological samples. In 2016 we developed a solid media transport card; for that pilot study to control the self-collection we used a pseudo-self-collection technique. The current study expands this prior work using true self-collections and only the POI card, and aims to evaluate the solid media transport card to detect HR-HPV in self-samples compared to liquid transport media. Methods Ten thousand eight hundred eighty-five women between the ages of 30–59 with no screening for 3 years were enrolled. The self-collected sample was first applied to a new solid media transport card (Labeled as SC) then the brush placed in 6 ml ThinPrep liquid (Labeled as SL). Then a physician collected a direct endocervical specimen into ThinPrep liquid (Labeled as DL). Samples were tested with Cobas 4800 and the SeqHPV NGS assay for HR-HPV. Patients positive on any test were recalled for colposcopy and biopsy. Results Ten thousand three hundred thirty-nine participants had complete data. The mean age was 43.9 years. CIN 2+ rates were 1.4% (142/10339). The agreement in HPV detection between the two different self-sample collection media was also good (Cobas HPV kappa = 0.86; SeqHPV kappa = 0.98). Tested with Cobas, the sensitivity of Cobas-SL and Cobas-SC for CIN 2+ was95.07 and 94.37%; and for CIN3+ was 96.30, 96.30% respectively. The specificity of Cobas-SC, and Cobas-SL for CIN2+ was 88.74 and 87.35%; for CIN3 was 88.04and 86.65% respectively. Tested with SeqHPV, the sensitivity for CIN2+ of Seq-SC and Seq-SL was 95.77 and 96.48%; for CIN3+, both the SC and SL specimens had a sensitivity of 100%. The specificity for CIN2+ of Seq-SC and Seq-SL was 89.54 and 89.53%; for CIN3+ was 88.84,88.82% respectively. For both HR-HPV assays, the sensitivities were similar for the two self-sample media (SC vs SL, p = 1.00). Conclusions The solid transport card for collecting vaginal self-samples as accurate as liquid transport media assayed by two different PCR based HR-HPV tests. The solid transport media is a suitable medium for collecting and storing vaginal self-samples.

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Section: Discussionmentioning

confidence: 63%

An evaluation of solid versus liquid transport media for high-risk HPV detection and cervical cancer screening on self-collected specimens

Duan

Yan

et al. 2020

Infect Agents Cancer

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“…Since the stability and integrity of viral RNA for molecular detection is a significant concern during fieldwork, this systematic review focused on the evaluation of FTA cards® as a strategy for detection of these pathogens. However, successful applications of these cards have been also reported for DNA viruses and bacteria (Dong et al, 2017;Reeve et al, 2018). For instance, the use of FTA cards® have been proved effective as a means of sample storage and transport for genotyping isolates of Campylobacter jejuni obtained from poultry and stored for three months at room temperature (Sierra-Arguello et al, 2016).…”

Section: Limitationsmentioning

confidence: 99%

“…FTA cards® have also been used for storing samples and molecular detection of both DNA and RNA viruses. Recently, they have been proposed as an alternative to liquid media in the screening of cervical cancer for HPV detection (Dong et al, 2017), and improved biosafety has been cited as an advantage in the storage and transport of specimens containing avian influenza virus (Kraus et al, 2011). However, viral RNA is an unstable molecule, susceptible to ubiquitous RNases, complicating its transport and detection.…”

mentioning

confidence: 99%

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

Cardona-Ospina

Villalba-Miranda

Palechor-Ocampo

et al. 2019

Preventive Veterinary Medicine

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A B S T R A C TBackgroundDetection and characterization of viral RNA pathogens from fieldwork are challenging due to the instability of the RNA molecule. FTA cards® have proved useful for sample storage and latter identification of pathogens with importance for agricultural, animal and human health: however, for optimal handling, processing, and biosafety measures are not well-established.ObjectiveThis systematic review aims to summarize the reported effectiveness of FTA cards® for storage and transport of viral RNA, as well as the conditions for their handling and use in downstream processes. Finally, the biosafety measures required to protect researchers and clinical lab workers are considered.MethodsWe performed a systematic review following the PRISMA statement. We searched MEDLINE (PubMed), Scopus and Web of Science using the keywords "FTA cards" AND "RNA". Articles were screened by title and abstract, and after examination of inclusion and exclusion criteria, relevant information was extracted. The quality of the studies was assessed, and the evidence was qualitatively summarized.ResultsA total of 175 records were retrieved, and 11 additional documents were found by checking references of the eligible articles. A total of 47 articles were included. Samples from animals accounted for 38.3% of the publications, which identified viruses that cause disease in poultry, wild birds, suids, or bovids. Three different methods for RNA extraction were reported. Other factors that vary across reports include the size of RNA amplicon, storage temperature, and duration of storage. Only fourteen articles tested the inactivation of the virus on the FTA card®, and in one case, the virus remained infective.ConclusionFTA cards® could be a suitable option for RNA virus storage and transport for fieldwork in areas where proper conditions for RNA preservation are difficult to achieve. Three different protocols have been used for RNA detection from this matrix. Biospecimens in the form of dried blood spots should be considered potentially infectious unless specifically treated to inactivate viral pathogens. technology consists of filter paper impregnated with a patented chemical mixture that contains chemical denaturants and a free radical scavenger. The chemical mixture lyses cells and organelles, prevents overgrowth of bacteria, and denatures proteins. The DNA is captured in the matrix and remains tightly bound while proteins and inhibitors are washed. This keeps DNA stable during long-term storage at room temperature (GE Healthcare, 2019).

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“…We surmised that self-sampling of the oral cavity can be introduced using FTA technology, as shown by repeated sampling of patients with potentially malignant cervical lesions, which has been proven to be practical and well accepted (34)(35)(36). Furthermore, dry FTA cell sampling technology is an easy system to use in primary care, an effective, reliable and sensitive method for transporting and storing of specimens (37).…”

Section: Discussionmentioning

confidence: 99%

Brush Samples of Oral Lesions to FTA Elute Card for High-risk Human Papilloma Virus Diagnosis

Stark¹,

Gustavsson

Horal

et al. 2021

Anticancer Res

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An evaluation of clinical performance of FTA cards for HPV 16/18 detection using cobas 4800 HPV Test compared to dry swab and liquid medium

Cited by 9 publications

References 28 publications

An evaluation of solid versus liquid transport media for high-risk HPV detection and cervical cancer screening on self-collected specimens

An evaluation of solid versus liquid transport media for high-risk HPV detection and cervical cancer screening on self-collected specimens

A systematic review of FTA cards® as a tool for viral RNA preservation in fieldwork: Are they safe and effective?

Brush Samples of Oral Lesions to FTA Elute Card for High-risk Human Papilloma Virus Diagnosis

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