An evaluation of a gel technique for antibody screening compared with a conventional tube method

Pinkerton, P. H.; Ward, J.; Chan, Raymond; Coovadia, Ahmed S.

doi:10.1111/j.1365-3148.1993.tb00116.x

Cited by 24 publications

(22 citation statements)

References 11 publications

(7 reference statements)

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“…(1992) who found DiaMed‐ID to be less sensitive than LISS‐IAT in detecting weak anti‐Fy b , anti‐Jk a and anti‐S. In an evaluation conducted by Pinkerton et al . (1993) of 39 sera, comparing DiaMed‐ID with NISS‐IAT (serum to cell ratio of between 120 : 1 and 200 : 1), several examples of anti‐Jk a required additional complement before they were detectable by DiaMed‐ID, and detectable examples gave a lower titre with this technology.…”

Section: Discussionsupporting

confidence: 72%

The United Kingdom National External Quality Assessment Scheme (Blood Transfusion Laboratory Practice): trends in proficiency and practice between 1985 and 2000

Knowles¹,

Milkins²,

Chapman

et al. 2002

Transfusion Medicine

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Proficiency in blood transfusion laboratory practice has improved over the last 15 years (1985-2000). Error rates for ABO grouping have fallen from 0 x 19 to 0 x 02% (P = 0 x 003). A similar trend is evident for antibody screening, with error rates for false negative antibody screens falling from 3 x 2 to 0 x 5% (P < 0 x 001), and for antibody identification, for sera containing a single alloantibody, with error rates falling from 8 x 8% to 0 x 9% over the last 10 years (P < 0 x 001). Proficiency in serological crossmatching to detect clinically significant non-ABO incompatibilities (other than Kidd), has also improved (P < 0 x 001). However, error rates for RhD grouping have not changed and there has been a recent decline in proficiency in detecting weak ABO incompatibilities and Kidd antibodies with heterozygous cells. Procedures for pretransfusion testing have also been rationalized over this time. The indirect antiglobulin test (IAT) is used in isolation by 73% of laboratories for antibody screening, and 10% rely on the direct room temperature (DRT), immediate spin (IS) crossmatch in the absence (current and/or historical) of clinically significant antibodies. Despite the improvements in error rates that have occurred alongside the rationalizations, there is still evidence of noncompliance with current published BCSH guidelines and manufacturers' instructions.

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Section: Discussionsupporting

confidence: 72%

The United Kingdom National External Quality Assessment Scheme (Blood Transfusion Laboratory Practice): trends in proficiency and practice between 1985 and 2000

Knowles¹,

Milkins²,

Chapman

et al. 2002

Transfusion Medicine

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“…Some workers found a higher sensitivity in the microtube column systems compared to the tube test (Bromilow et al , 1991; Voak, 1999; Weisbach et al , 1999). However, other investigators found the microtube column systems to be slightly less sensitive than the tube test (Phillips et al , 1992; Voak, 1992; Pinkerton et al , 1993). This discrepancy is most likely due to different characteristics of tube tests, which were compared to the gel test (Issitt & Anstee, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…This discrepancy is most likely due to different characteristics of tube tests, which were compared to the gel test (Issitt & Anstee, 1998). For example, Pinkerton et al (1993) used a tube saline IAT with a comparably high ratio of serum to red cells between 120 : 1 and 200 : 1 combined with a microscopic confirmation of negative results. The new methods have important advantages compared to the tube test: they are less susceptible to handling errors, reading of results can be repeated, and, most important, the new methods can be performed automatically.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the performance of microtube column systems and solid‐phase systems and the tube low‐ionic‐strength solution additive indirect antiglobulin test in the detection of red cell alloantibodies

Weisbach

Kohnhäuser

Zimmermann

et al. 2006

Transfusion Medicine

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To compare the performance of seven currently available test systems in the detection of erythrocyte alloantibodies (ab), we tested in parallel 446 sera samples containing red cell ab [368 sera samples with ab that are assumed to be clinically significant (cs-ab) and 78 sera samples with ab that are assumed to be of minor clinical significance (ms-ab)] using the tube spin low-ionic-strength solution (addition method) indirect antiglobulin test (tube LISS-IAT), three microtube column agglutination techniques (DiaMed-ID, Ortho BioVue and Bio-Rad Scangel), one affinity adherence test system (CLB/Mast CellBind Screen) and two solid-phase tests [Biotest Solidscreen II and Immucor Capture-R Ready-Screen (4)]. To address the specificity of the three test systems under routine conditions, results of 4566 patient samples obtained using the tube LISS-IAT, results of 5205 patient samples obtained using the Scangel and results of 3560 samples obtained using the Capture-R were evaluated. The DiaMed-ID detected 344 cs-ab and 43 ms-ab, BioVue 333 cs-ab and 48 ms-ab, Scangel 348 cs-ab and 62 ms-ab, CellBind Screen 346 cs-ab and 47 ms-ab, Solidscreen 330 cs-ab and 38 ms-ab, Capture-R 358 cs-ab and 45 ms-ab and LISS-IAT 159 cs-ab and 12 ms-ab. In routine practice, erythrocyte cs-ab could be identified in 61 (67.8%) of 90 reactive sera (specificity: 98.6%) in the tube LISS-IAT, in 169 (58.7%) of 288 (94.4%) in Bio-Rad Scangel and in 101 (51.0%) of 198 reactive sera (94.3%) in Capture-R. We conclude that the sensitivity of the microcolumn, affinity adherence and solid-phase test systems in the detection of cs-ab was similar and was markedly superior to that of the conventional tube LISS-IAT. All high-sensitive test systems produced higher rates of false positives and ms-ab compared to the tube test. An individual cost-benefit analysis, considering the recent knowledge about the clinical significance of weak-reactive cs-ab, should be performed in every institution to decide whether and if so which high-sensitive screening system should be applied.

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“…However, the Dal antigen investigators utilized a gel technology in conjunction with a standard tube technology for crossmatching and reported agreement of both methods 11 . In human medicine the gel test is comparable to the tube assay for both the indirect and direct antiglobulin tests 23–26 . There are currently 2 commercially available gel tests for canine and feline crossmatching: one manufactured by DiaMed and one manufactured by DMS Laboratories e,f (Figure 4).…”

Section: Crossmatching Methodsmentioning

confidence: 99%

Increasing patient safety in veterinary transfusion medicine: an overview of pretransfusion testing

Tocci¹,

Ewing

2009

J Vet Emergen Crit Care

122

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Objectives -To review the principles and available technology for pretransfusion testing in veterinary medicine and discuss the indications and importance of test performance before RBC transfusion. Data Sources -Current human and veterinary medical literature: original research articles and scientific reviews. Summary -Indications for RBC transfusion in veterinary medicine include severe anemia or tissue hypoxia resulting from blood loss, decreased erythrocyte production, and hemolyzing conditions such as immunemediated anemia and neonatal isoerythrolysis. Proper blood sample collection, handling, and identification are imperative for high-quality pretransfusion testing. Point-of-care blood typing methods including both typing cards and rapid gel agglutination are readily available for some species. Following blood typing, crossmatching is performed on one or more donor units of appropriate blood type. As an alternative to technically demanding tube crossmatching methods, a point-of-care gel agglutination method has recently become available for use in dogs and cats. Crossmatching reduces the risk of hemolytic transfusion reactions but does not completely eliminate the risk of other types of transfusion reactions in veterinary patients, and for this reason, all transfusion reactions should be appropriately documented and investigated. Conclusion -The administration of blood products is a resource-intensive function of veterinary medicine and optimizing patient safety in transfusion medicine is multifaceted. Adverse reactions can be life threatening. Appropriate donor screening and collection combined with pretransfusion testing decreases the occurrence of incompatible transfusion reactions.

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An evaluation of a gel technique for antibody screening compared with a conventional tube method

Cited by 24 publications

References 11 publications

The United Kingdom National External Quality Assessment Scheme (Blood Transfusion Laboratory Practice): trends in proficiency and practice between 1985 and 2000

The United Kingdom National External Quality Assessment Scheme (Blood Transfusion Laboratory Practice): trends in proficiency and practice between 1985 and 2000

Comparison of the performance of microtube column systems and solid‐phase systems and the tube low‐ionic‐strength solution additive indirect antiglobulin test in the detection of red cell alloantibodies

Increasing patient safety in veterinary transfusion medicine: an overview of pretransfusion testing

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