An evaluation of a cultured human corneal epithelial tissue model for the determination of the ocular irritation potential of pharmaceutical process materials

Seaman, Christopher; Whittingham, Andrew; Guest, Robert; Warren, N.; Olson, Michael A.; Guerriero, Frederick J.; Adriaens, Els; Wever, Bart De

doi:10.1016/j.tiv.2010.03.004

Cited by 10 publications

(5 citation statements)

References 12 publications

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“…As a further approach, 3D cornea epithelial models have been reconstructed (Doucet et al, 2006;Van Goethem et al, 2006;Huhtala et al, 2008;Seaman et al, 2010;Kaluzhny et al, 2011;Kolle et al, 2011;Katoh et al, 2013). Approaches using 3D epithelial models only discriminate between irritants (I) and non-irritants (NI) based on a classification (PM) with one viability cut-off value (Van Goethem et al, 2006;Kaluzhny et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Development of an in vitro ocular test system for the prediction of all three GHS categories

Bartok

Gabel

Zorn-Kruppa

et al. 2015

Toxicology in Vitro

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Section: Introductionmentioning

confidence: 99%

Development of an in vitro ocular test system for the prediction of all three GHS categories

Bartok

Gabel

Zorn-Kruppa

et al. 2015

Toxicology in Vitro

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“…Furthermore, approaches have been published that take advantage of the reactions evoked by chemicals in incubated hen eggs [7] or invertebrates [13] , [14] . Also a test system based on the perturbation and denaturation of corneal proteins which is supposed to mimic the disruptive effect of ocular irritants [15] and various 3D cornea epithelial models [16] , [17] , [18] , [19] , [20] , [21] , [22] have been developed. Currently some methods have gained regulatory acceptance for selected GHS categories.…”

Section: Introductionmentioning

confidence: 99%

Determining the Depth of Injury in Bioengineered Tissue Models of Cornea and Conjunctiva for the Prediction of All Three Ocular GHS Categories

et al. 2014

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The depth of injury (DOI) is a mechanistic correlate to the ocular irritation response. Attempts to quantitatively determine the DOI in alternative tests have been limited to ex vivo animal eyes by fluorescent staining for biomarkers of cell death and viability in histological cross sections. It was the purpose of this study to assess whether DOI could also be measured by means of cell viability detected by the MTT assay using 3-dimensional (3D) reconstructed models of cornea and conjunctiva. The formazan-free area of metabolically inactive cells in the tissue after topical substance application is used as the visible correlate of the DOI. Areas of metabolically active or inactive cells are quantitatively analyzed on cryosection images with ImageJ software analysis tools. By incorporating the total tissue thickness, the relative MTT-DOI (rMTT-DOI) was calculated. Using the rMTT-DOI and human reconstructed cornea equivalents, we developed a prediction model based on suitable viability cut-off values. We tested 25 chemicals that cover the whole range of eye irritation potential based on the globally harmonized system of classification and labelling of chemicals (GHS). Principally, the MTT-DOI test method allows distinguishing between the cytotoxic effects of the different chemicals in accordance with all 3 GHS categories for eye irritation. Although the prediction model is slightly over-predictive with respect to non-irritants, it promises to be highly valuable to discriminate between severe irritants (Cat. 1), and mild to moderate irritants (Cat. 2). We also tested 3D conjunctiva models with the aim to specifically address conjunctiva-damaging substances. Using the MTT-DOI method in this model delivers comparable results as the cornea model, but does not add additional information. However, the MTT-DOI method using reconstructed cornea models already provided good predictability that was superior to the already existing established in vitro/ex vivo methods.

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“…Furthermore, the use of nanotechnology for product development is a very innovative and constantly growing area. Additionally, it is worth mentioning the adoption of alternative methods to animal experimentation in pipelines of formulations and product development for the cosmetic industry (Seaman et al, 2010).…”

Section: Graphical Abstractmentioning

confidence: 99%

Biological activities of green coffee nanoemulsions evaluated through alternative methods: MTT, cellular proliferation, and HET-CAM assays

Buzanello,

Lopes,

Coelho

et al. 2023

Biotechnol. Res. Innov.

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An evaluation of a cultured human corneal epithelial tissue model for the determination of the ocular irritation potential of pharmaceutical process materials

Cited by 10 publications

References 12 publications

Development of an in vitro ocular test system for the prediction of all three GHS categories

Development of an in vitro ocular test system for the prediction of all three GHS categories

Determining the Depth of Injury in Bioengineered Tissue Models of Cornea and Conjunctiva for the Prediction of All Three Ocular GHS Categories

Biological activities of green coffee nanoemulsions evaluated through alternative methods: MTT, cellular proliferation, and HET-CAM assays

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