An ethylene response factor (ERF5) promoting adaptation to drought and salt tolerance in tomato

Pan, Yu; Seymour, Graham B.; Lu, Chungui; Hu, Zongli; Chen, Xüqing; Chen, Guoping

doi:10.1007/s00299-011-1170-3

Cited by 220 publications

(99 citation statements)

References 48 publications

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“…Supporting our observations, ERF6 was recently shown to be necessary for WRKY33 induction under oxidative stress (Wang et al, 2013). Consistent with the activation of stress tolerance genes by ERF5 and ERF6, overexpression of SlERF5 in tomato (Solanum lycopersicum) plants was recently shown to confer tolerance to drought stress (Pan et al, 2012). ERF6 regulates two diverse processes: on the one hand, the activation of the stress defense transcriptional cascade, and on the other hand, the regulation of growth inhibition.…”

Section: Erfs Regulate Many Stress Resistance Genes In a Ga/della-indsupporting

confidence: 84%

ETHYLENE RESPONSE FACTOR6 Acts as a Central Regulator of Leaf Growth under Water-Limiting Conditions in Arabidopsis

et al. 2013

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Leaf growth is a complex developmental process that is continuously fine-tuned by the environment. Various abiotic stresses, including mild drought stress, have been shown to inhibit leaf growth in Arabidopsis (Arabidopsis thaliana), but the underlying mechanisms remain largely unknown. Here, we identify the redundant Arabidopsis transcription factors ETHYLENE RESPONSE FACTOR5 (ERF5) and ERF6 as master regulators that adapt leaf growth to environmental changes. ERF5 and ERF6 gene expression is induced very rapidly and specifically in actively growing leaves after sudden exposure to osmotic stress that mimics mild drought. Subsequently, enhanced ERF6 expression inhibits cell proliferation and leaf growth by a process involving gibberellin and DELLA signaling. Using an ERF6-inducible overexpression line, we demonstrate that the gibberellin-degrading enzyme GIBBERELLIN 2-OXIDASE6 is transcriptionally induced by ERF6 and that, consequently, DELLA proteins are stabilized. As a result, ERF6 gain-of-function lines are dwarfed and hypersensitive to osmotic stress, while the growth of erf5erf6 loss-of-function mutants is less affected by stress. Besides its role in plant growth under stress, ERF6 also activates the expression of a plethora of osmotic stress-responsive genes, including the well-known stress tolerance genes STZ, MYB51, and WRKY33. Interestingly, activation of the stress tolerance genes by ERF6 occurs independently from the ERF6-mediated growth inhibition. Together, these data fit into a leaf growth regulatory model in which ERF5 and ERF6 form a missing link between the previously observed stress-induced 1-aminocyclopropane-1-carboxylic acid accumulation and DELLA-mediated cell cycle exit and execute a dual role by regulating both stress tolerance and growth inhibition.

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Section: Erfs Regulate Many Stress Resistance Genes In a Ga/della-indsupporting

confidence: 84%

ETHYLENE RESPONSE FACTOR6 Acts as a Central Regulator of Leaf Growth under Water-Limiting Conditions in Arabidopsis

et al. 2013

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“…This result suggests, on the one hand, that ERF1 might regulate ROS signaling and on the other hand, that Pro accumulation seems to be a common response of ERFs to stress. This is, indeed, documented for the majority of TFs from the ERF subfamily that enhance abiotic stress tolerance, such as JERF1 , TSRF1 (Quan et al, 2010), GmERF3 (Zhang et al, 2009), Tomato Ethylene-Response Factor5 (Pan et al, 2012), CsERF , JcERF1 , LeERF1, LeERF2 , MsERF8 , LcERF054, LcERF080 (Sun et al, 2014a(Sun et al, , 2014b, LchERF , and TaERF3 (Rong et al, 2014). Overexpression of LeERF1, LeERF2, and MsERF8 has been reported to elevate Pro accumulation and reduce malondialdehyde levels, an indicator of lipid peroxidation, in tomato and tobacco plants under salt stress (Cheng et al, 2013;Hu et al, 2014).…”

Section: Erfs and Redox Signalingmentioning

confidence: 71%

Ethylene Response Factors: A Key Regulatory Hub in Hormone and Stress Signaling

2015

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ORCID IDs: 0000-0001-7494-6841 (M.M.); 0000-0001-6523-6848 (S.M.-B.).Ethylene is essential for many developmental processes and a key mediator of biotic and abiotic stress responses in plants. The ethylene signaling and response pathway includes Ethylene Response Factors (ERFs), which belong to the transcription factor family APETALA2/ERF. It is well known that ERFs regulate molecular response to pathogen attack by binding to sequences containing AGCCGCC motifs (the GCC box), a cis-acting element. However, recent studies suggest that several ERFs also bind to dehydration-responsive elements and act as a key regulatory hub in plant responses to abiotic stresses. Here, we review some of the recent advances in our understanding of the ethylene signaling and response pathway, with emphasis on ERFs and their role in hormone cross talk and redox signaling under abiotic stresses. We conclude that ERFs act as a key regulatory hub, integrating ethylene, abscisic acid, jasmonate, and redox signaling in the plant response to a number of abiotic stresses.

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“…When we predicted the protein structure and obtained the interaction between our ERF1 and other ERFs proteins in the Solaneace family, we found high similarity to TERF1 which suggested similar protein function. Similarly, Pan et al (2012) reported that overexpression of SlERF5 in transgenic tomato plants resulted in high tolerance to drought and salt stress and increased levels of relative water content. When we predicted the protein structure and obtained the interaction between our Edkawy ERF5 and other ERFs proteins in the Solaneace family, we found one SNP between our ERF5 and SlERF5 proteins.…”

Section: Resultsmentioning

confidence: 93%

Molecular characterization of two AP2/ERF transcription factor genes from Egyptian tomato cultivar (Edkawy)

Mosa

El-din²,

Ismail³

et al. 2017

Plant Sci. Today

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The tomato is ranked first amongst vegetable crops in Egypt in relation to surface area and production. The Egyptian tomato cultivar Edkawy has shown abiotic stress tolerance characteristics. However, there is not much information about the molecular characterization of this cultivar. Furthermore, information regarding the identification of abiotic stress tolerance genes from the Edkawy tomato cultivar is lacking. Here, we investigated the ability of the Edkawy cultivar to tolerate drought stress. Two varieties were used as a control in this study; Peto86 (sensitive variety) and Strain B (tolerant variety). Edkawy, Peto86 and Strain B varieties were exposed to drought stress by reducing the water supply gradually. Interestingly, Edkawy demonstrated a remarkable tolerance phenotype to drought stress. Furthermore, we identified and isolated two members of the AP2/ERF transcription factor family from Edkawy which are associated with abiotic stress, particularly drought, i.e. ERF1 and ERF5. Protein prediction, validation and active site prediction of ERF1 and ERF5 were also determined. In addition to the domain obtained by the pfam online tool, the interaction between Edkawy ERFs proteins and other proteins in the Solanaceae family was obtained. Furthermore, subcellular localization was determined by the ngLOC and Plant-mPLoc online tools. Characterization of the Edkawy tomato cultivar and isolation and identification of such transcription factors will help in the engineering of tomato plants with abiotic stress tolerance.

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An ethylene response factor (ERF5) promoting adaptation to drought and salt tolerance in tomato

Cited by 220 publications

References 48 publications

ETHYLENE RESPONSE FACTOR6 Acts as a Central Regulator of Leaf Growth under Water-Limiting Conditions in Arabidopsis

ETHYLENE RESPONSE FACTOR6 Acts as a Central Regulator of Leaf Growth under Water-Limiting Conditions in Arabidopsis

Ethylene Response Factors: A Key Regulatory Hub in Hormone and Stress Signaling

Molecular characterization of two AP2/ERF transcription factor genes from Egyptian tomato cultivar (Edkawy)

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