An ethnobotanical survey and inhibitory effects on NLRP3 inflammasomes/Caspase-1 of herbal recipes’ extracts traditionally used in Rwanda for asthma treatment

Tomani, Jean Claude Didelot; Gainkam, Lea Olive Tchouate; Nshutiyayesu, Samuel; Mukazayire, Marie-Jeanne; Ribeiro, Sofia Oliveira; Stévigny, Caroline; Frédérich, Michel; Muganga, Raymond; Souopgui, Jacob

doi:10.1016/j.jep.2018.08.016

Cited by 18 publications

(16 citation statements)

References 62 publications

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“…Hibiscus noldeae ( H. noldeae ) has been studied by virtue of its attribute as one of the medicinal plants used by the traditional healers in Rwanda to manufacture their remedies. In the context of asthma treatment, the recipe, containing 7 different plants, that revealed the best anti-inflammatory activity [ 21 ] comprised H. noldeae in major proportion (35%). We therefore hypothesized that this medicinal plant may harbor the therapeutic constituents.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous study, among the plant recipes evaluated, R19Sz was the most potent in inhibiting caspase-1 activity [ 21 ]. Hibiscus noldeae , one of the plant’s components of the recipe, has been cited by three different traditional healers during our investigation, indicating its possible importance in the treatment of asthma.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, few studies reported on the traditional use of the plant in the treatment of different ailments, some of which are of inflammatory background such as asthma, wounds, headache, etc. [ 21 , 27 , 28 , 29 ]. An important question was then to determine whether H. noldeae , would have inhibitory properties on inflammasome or on IL-6 production, a well-known downstream target of IL-1β.…”

Section: Discussionmentioning

confidence: 99%

“…A reliable therapeutic source which remains poorly explored is the traditional pharmacopeia. Previously, our investigations on the anti-asthmatic activities of herbal recipes from Rwandese traditional healers revealed a candidate recipe with significant inhibitory properties on the activation of caspase-1 using Caspase-Glo 1 Inflammasome assays [ 21 ]. Hibiscus noldeae , from the Hibiscus genus in the Malvaceae family, was one of the plants composing the recipe.…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, no such studies has been reported on H. noldeae , although used by traditional healers in the treatment of asthma, urogenital infections, postpartum abdominal pain, swelling of the legs, cleaning of babies, facilitation of delivery, sprains, headache, wounds, women infertility, abortion prevention, etc. [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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The Inhibition of NLRP3 Inflammasome and IL-6 Production by Hibiscus noldeae Baker f. Derived Constituents Provides a Link to Its Anti-Inflammatory Therapeutic Potentials

et al. 2020

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The activation of NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome and/or its components is associated with the physio-pathogenesis of many respiratory diseases including asthma, COPD (chronic obstructive pulmonary disease), SARS Cov-2 (severe acute respiratory syndrome coronavirus 2), and in several autoimmune diseases. Hibiscus noldeae Baker f. has been widely reported to be traditionally used in the treatment of different ailments, some of which are of inflammatory background such as asthma, wounds, headache, etc. However, the claims have not been supported by evidence at the molecular and functional levels. Here, we report on the bio-guided fractionation of H. noldeae and assessment of the inhibitory properties of some fractions and purified compounds on NLRP3 inflammasome and Interleukin 6 (IL-6). The activation of the NLRP3 inflammasome was determined by detecting the activity of caspase-1 and the production of Interleukin 1β (IL-1β) in Lipopolysaccharide (LPS) and ATP-stimulated Tamm-Horsfall Protein 1 (THP-1) macrophages, while the production of IL-6 was studied in LPS-stimulated RAW264.7 mouse macrophages. It was observed that hexane and ethyl acetate fractions of the crude extract of the aerial parts of H. noldeae, as well as caffeic acid, isoquercetin, and ER2.4 and ER2.7 fractions revealed significant inhibitory effects on Caspase-1 activities, and on IL-1β and IL-6 production. The ER2.4 and ER2.7 fractions downregulated the production of IL-1β and IL-6, in a similar range as the caspase-1 inhibitor AC-YVAD-CHO and the drug Dexamethasone, both used as controls, respectively. Overall, our work does provide the very first scientific based evidence for Hibiscus noldeae anti-inflammatory effects and widespread use by traditional healers in Rwanda for a variety of ailments.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Inhibition of NLRP3 Inflammasome and IL-6 Production by Hibiscus noldeae Baker f. Derived Constituents Provides a Link to Its Anti-Inflammatory Therapeutic Potentials

et al. 2020

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Macrophage efferocytosis in health and disease

Razi

Khojini

Kargarijam

et al. 2023

Cell Biochemistry & Function

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Creating cellular homeostasis within a defined tissue typically relates to the processes of apoptosis and efferocytosis. A great example here is cell debris that must be removed to prevent unwanted inflammatory responses and then reduce autoimmunity. In view of that, defective efferocytosis is often assumed to be responsible for the improper clearance of apoptotic cells (ACs). This predicament triggers off inflammation and even results in disease development. Any disruption of phagocytic receptors, molecules as bridging groups, or signaling routes can also inhibit macrophage efferocytosis and lead to the impaired clearance of the apoptotic body. In this line, macrophages as professional phagocytic cells take the lead in the efferocytosis process. As well, insufficiency in macrophage efferocytosis facilitates the spread of a wide variety of diseases, including neurodegenerative diseases, kidney problems, types of cancer, asthma, and the like. Establishing the functions of macrophages in this respect can be thus useful in the treatment of many diseases. Against this background, this review aimed to recapitulate the knowledge about the mechanisms related to macrophage polarization under physiological or pathological conditions, and shed light on its interaction with efferocytosis.

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Biological evaluation of imidazopyridine derivatives as potential anticancer agents against breast cancer cells

Sucu

2022

Med Chem Res

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An ethnobotanical survey and inhibitory effects on NLRP3 inflammasomes/Caspase-1 of herbal recipes’ extracts traditionally used in Rwanda for asthma treatment

Cited by 18 publications

References 62 publications

The Inhibition of NLRP3 Inflammasome and IL-6 Production by Hibiscus noldeae Baker f. Derived Constituents Provides a Link to Its Anti-Inflammatory Therapeutic Potentials

The Inhibition of NLRP3 Inflammasome and IL-6 Production by Hibiscus noldeae Baker f. Derived Constituents Provides a Link to Its Anti-Inflammatory Therapeutic Potentials

Macrophage efferocytosis in health and disease

Biological evaluation of imidazopyridine derivatives as potential anticancer agents against breast cancer cells

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