1975
DOI: 10.1093/jnci/55.5.1155
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An Ether-Sensitive Antigen Associated With Bovine Leukemia Virus Infection2

Abstract: An ether-sensitive antigen (es-Ag) associated with bovine leukemia virus infection was detected by immunodiffusion tests. This antigen was sensitive to ether, sodium periodate, and trypsin treatment. Based on column chromatography, es-Ag was a larger molecule than that of ether-resistant antigen (gs-Ag). With infection, precipitating antibody against es-Ag developed earlier than the precipitating antibody against the gs-Ag.

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Cited by 63 publications
(18 citation statements)
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“…Anti-BIV and -BLV antibodies were detected in those sera by Western blot analysis using the BIV gag protein, p26 [12][13][14][15][16], and immunodiffusion test using the BLV glycoprotein (gp51) antigen as described by Onuma et al [19], respectively. As summarized in Table 1, the prevalence of BIV in dairy cattle from each district ranged from 0.0 to 25.0% (average 11.0%), whereas BLV ranged from 0.0 to 13.3% (average 3.3%).…”
mentioning
confidence: 99%
“…Anti-BIV and -BLV antibodies were detected in those sera by Western blot analysis using the BIV gag protein, p26 [12][13][14][15][16], and immunodiffusion test using the BLV glycoprotein (gp51) antigen as described by Onuma et al [19], respectively. As summarized in Table 1, the prevalence of BIV in dairy cattle from each district ranged from 0.0 to 25.0% (average 11.0%), whereas BLV ranged from 0.0 to 13.3% (average 3.3%).…”
mentioning
confidence: 99%
“…As summarized in Table 1, 10.3% of buffaloes and 15.8% of cattle tested were found seropositive for BIV infection. To demonstrate the correlation between BIV and BLV infections, these samples were also analyzed for anti-BLV antibodies by immunodiffusion test using the BLV glycoprotein antigen as described by Onuma et al [19]. Few animals were found seropositive for BLV (0.8% in buffaloes, 0% in cattle).…”
mentioning
confidence: 99%
“…For detecting antibodies against BLV, the immunodiffusion test with glycoprotein as antigen and the complement-fixation test were performed as described previously (17) .…”
mentioning
confidence: 99%