An ESAT-6:CFP10 DNA vaccine administered in conjunction with Mycobacterium bovis BCG confers protection to cattle challenged with virulent M. bovis

Maue, Alexander C.; Waters, W. Ray; Palmer, Mitchell V.; Nonnecke, Brian J.; Minion, F. Chris; Brown, Wendy C.; Norimine, Junzo; Foote, Monica R.; Scherer, Charles Fernando Capinos; Estes, D. Mark

doi:10.1016/j.vaccine.2007.03.052

Cited by 51 publications

(37 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MPB64 had been considered important immunodominant antigens encoded by RD1and RD2 of M. tuberculosis, respectively (Maue et al, 2007). Recent study demonstrated that DNA vaccine expressing the gene of MPB64 could strength protection efficacy, which was complied with our results (Kaufmann et al, 2011).…”

Section: Tuberculosis (Tb) Is a Common And Sometimes Lethal Infectiousupporting

confidence: 87%

“…The MPB64 gene, encoded by RD1, has been investigated extensively, and has been shown to have great potential in the specific in vitro diagnosis of MTB infection in human beings (Maue et al, 2007;Kaufmann et al, 2011). Previous studies showed that RDs encode some important immuno-dominant antigens of M. tuberculosis and RD1 could enhance the protective efficacy of BCG in the forms of recombinant BCG or subunit vaccine.…”

Section: Tuberculosis (Tb) Is a Common And Sometimes Lethal Infectioumentioning

confidence: 99%

See 1 more Smart Citation

MPB64 DNA vaccine with immunogenicity and efficacy against tuberculosis

Yao¹,

Hou²,

Jiang³

et al. 2012

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Tuberculosis is a serious infectious disease caused by Mycobacterium tuberculosis. DNA vaccination is an advanced technique for protecting human bodies from infectious diseases including tuberculosis by injecting exogenous gene engineering DNA into the body to produce an immunological response. In this study, we examined the immunogenicity and protective efficacy of DNA vaccine (pCDNA-MPB64) expressing MPB64 protein and its booster effects in mice for controlling tuberculosis. The results showed that MPB64 DNA vaccine led to a dramatic augmentation of humoral and cellular responses. All these suggested that MPB64 DNA vaccines is an ideal vaccine and may be further developed as a useful method to prevent tuberculosis.

show abstract

Section: Tuberculosis (Tb) Is a Common And Sometimes Lethal Infectiousupporting

confidence: 87%

Section: Tuberculosis (Tb) Is a Common And Sometimes Lethal Infectioumentioning

confidence: 99%

MPB64 DNA vaccine with immunogenicity and efficacy against tuberculosis

Yao¹,

Hou²,

Jiang³

et al. 2012

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…In vitro, bovine gd T cells proliferate and produce IFN-g in recall responses to complex Ags, such as PPD-B (17,18), and to specific Ags, such as the protein complex ESAT6:CFP10 (E:C) (17,19), and the nonprotein Ag mycolylarabinogalactan peptidoglycan (mAGP), a component of the mycobacterial cell wall (20). From these studies, it is clear that gd T cells respond to mycobacterial infection; however, much remains to be understood about the specific Ags and Ag-presenting molecules mediating this recognition.…”

mentioning

confidence: 99%

Specific Recognition of Mycobacterial Protein and Peptide Antigens by γδ T Cell Subsets following Infection with VirulentMycobacterium bovis

McGill

Sacco

Baldwin

et al. 2014

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

Promoting effective immunity to Mycobacterium bovis infection is a challenge that is of interest to the fields of human and animal medicine alike. We report that γδ T cells from virulent M. bovis–infected cattle respond specifically and directly to complex, protein, and nonprotein mycobacterial Ags. Importantly, to our knowledge, we demonstrate for the first time that bovine γδ T cells specifically recognize peptide Ags derived from the mycobacterial protein complex ESAT6:CFP10 and that this recognition requires direct contact with APCs and signaling through the T cell Ag receptor but is independent of MHC class I or II. Furthermore, we show that M. bovis infection in cattle induces robust IL-17A protein responses. Interestingly, in contrast to results from mice, bovine CD4 T cells, and not γδ T cells, are the predominant source of this critical proinflammatory mediator. Bovine γδ T cells are divided into subsets based upon their expression of Workshop Cluster 1 (WC1), and we demonstrate that the M. bovis–specific γδ T cell response is composed of a heterogeneous mix of WC1-expressing populations, with the serologically defined WC1.1+ and WC1.2+ subsets responding in vitro to mycobacterial Ags and accumulating in the lesions of M. bovis–infected animals. The results described in this article enhance our understanding of γδ T cell biology and, because virulent M. bovis infection of cattle represents an excellent model of tuberculosis in humans, contribute to our overall understanding of the role of γδ T cells in the mycobacterial-specific immune response.

show abstract

“…DNA vaccines have induced minimal protection against TB when used alone, although some protection has been observed when mycobacterial DNA was combined with DNA encoding co-stimulatory molecules CD80 and CD86 [67] or combined with an adjuvant [68]. More encouraging results have been reported when DNA vaccines have been used in heterologous prime-boost regimes with BCG, and priming or boosting with mycobacterial DNA vaccines induced greater protection than with BCG vaccine alone [44,69,70]. Similarly, TB protein vaccines have induced little protection in cattle when used alone, whereas when coadministered at adjacent sites with BCG have induced protection that was better than that observed with BCG alone [45,71].…”

Section: New Tb Vaccines Evaluated In Cattlementioning

confidence: 99%

Immunity and Vaccination against Tuberculosis in Cattle

Parlane¹,

Buddle²

2015

Curr Clin Micro Rpt

View full text Add to dashboard Cite

Bovine tuberculosis (TB) continues to be a major animal health problem worldwide and new tools are required for the control of this disease in cattle, particularly with the emergence of wildlife reservoirs for Mycobacterium bovis infection and potential restrictions on trade. New insights into the roles of macrophages, dendritic cells, NK cells, and CD4+, CD8+, and γδ T cells in control of M. bovis infection in cattle have provided a better understanding of protective immunity against this disease. In the past two decades, considerable progress has been made in the development and evaluation of TB vaccines for cattle, with new attenuated mycobacterial vaccines providing an alternative to the use of BCG vaccine and sub-unit vaccines to boost protection induced by BCG. With the development of tests to differentiate infected from vaccinated animals, it is now feasible to use vaccines to assist in the control of this disease.

show abstract

An ESAT-6:CFP10 DNA vaccine administered in conjunction with Mycobacterium bovis BCG confers protection to cattle challenged with virulent M. bovis

Cited by 51 publications

References 59 publications

MPB64 DNA vaccine with immunogenicity and efficacy against tuberculosis

MPB64 DNA vaccine with immunogenicity and efficacy against tuberculosis

Specific Recognition of Mycobacterial Protein and Peptide Antigens by γδ T Cell Subsets following Infection with VirulentMycobacterium bovis

Immunity and Vaccination against Tuberculosis in Cattle

Contact Info

Product

Resources

About