An Epigallocatechin-3-gallate Formulation Developed for Endodontic Use: A Physicochemical and Biological Evaluation

Rossi, Andiara De; Ferreira, Danielly Cunha Araújo; Liévana, Fernanda Souza; Vilela, Marina Moscardini; Nelson‐Filho, Paulo; Silva, Roberto Santana da; Moraes, Juliana Cristina Biazzotto; Silva, Léa Assed Bezerra da

doi:10.1016/j.joen.2021.06.010

Cited by 4 publications

(8 citation statements)

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“…An EGCG derived from green tea and available on 5 μm provided in the solid-state (E41430; Sigma-Aldrich; St Louis, MO, USA) was used. The EGCG-based paste for endodontic use was prepared as previously reported 12 at a 1 mg/mL concentration using polyethylene glycol 400 (PEG 400, Galena Química e Farmacêutica Ltda., Campinas, SP, Brazil) as a vehicle and zinc oxide (2 g) (SS White Artigos Dentários Ltda., Rio de Janeiro, RJ, Brazil) as a radiopacity agent. A calcium hydroxide paste (Calen®, S.S. White Dental Articles Ltda.)…”

Section: Methodsmentioning

confidence: 99%

“…In endodontics, the use of EGCG has been suggested as a medication between sessions 12 ) or as an irrigant of root canals 13 . Due to its strong antioxidant properties, the use of EGCG as a final irrigant may neutralize any residual sodium hypochlorite and oxygen and increase the bond strength of root canal sealers 14 ) and bonding systems to root dentin 15 .…”

Section: Introductionmentioning

confidence: 99%

“…Due to its strong antioxidant properties, the use of EGCG as a final irrigant may neutralize any residual sodium hypochlorite and oxygen and increase the bond strength of root canal sealers 14 ) and bonding systems to root dentin 15 . Recently, studies carried out by our research group developed a formulation based on EGCG for endodontic use that presented tissue compatibility and promoted the healing of periapical tissues 12 , and the teeth treatment post radiotherapy with EGCG inactivates the enzymatic activity of MMPs activated by radiotherapy 16 . Thus, it appears that in addition to playing an anti-inflammatory, antioxidant, antimicrobial, and tissue regenerator role, intracanal medication with EGCG may inactivate MMPs.…”

Section: Introductionmentioning

confidence: 99%

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Effect of EGCG-based paste as intracanal dressing, in MMPs 2 and 9 expression in dog’s periapical lesions

Rossi,

Pradela,

Liévana

et al. 2024

Braz. Dent. J.

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High expression of MMP-2 and MMP-9 in periapical lesions plays an important role in the degradation of the extracellular matrix. This study aimed to investigate the effect of epigallocatechin-3-gallate (EGCG)-based endodontic paste as an intracanal dressing on the expression of MMP-2 and MMP-9 in periapical lesions. Periapical lesions were experimentally induced in 35 mature beagle dog premolars randomly divided into healthy teeth, untreated periapical lesions, periapical lesions treated in a single session (control groups), and periapical lesions treated in two sessions with EGCG or calcium hydroxide-based pastes (experimental groups). After 120 days, specimens were obtained for histopathologic and immunofluorescence analyses to assess the expression of MMP-2 and MMP-9. The statistical analysis was performed using a p-value of 0.05. Endodontic treatment in two sessions using medication with EGCG and calcium hydroxide-based pastes provided similar repair of the apical and periapical tissues and neoformation of periodontal ligament fibers, cementum, and alveolar bone (p>0.05). The experimental groups treated in two sessions with both medications presented expression of MMP-2 and MMP-9 similar to that in healthy teeth (p>0.05), and significantly lower than teeth treated in a single session or untreated periapical lesions (p <0.001). Expression of MMP-2 and MMP-9 was observed in the cytoplasm of fibroblasts, osteoblasts, cementoblasts, cementocytes, and vascular endothelium. The use of EGCG-based endodontic paste reduced the expression of MMP-2 and MMP-9 and allowed repair of periapical lesions, similar to calcium hydroxide-based paste, and superior to treatment performed in a single session.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of EGCG-based paste as intracanal dressing, in MMPs 2 and 9 expression in dog’s periapical lesions

Rossi,

Pradela,

Liévana

et al. 2024

Braz. Dent. J.

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show abstract

“…In endodontics, the use of EGCG has been suggested as a medication between sessions [17] or as an irrigant of root canals [18]. Due to its strong antioxidant properties, the use of EGCG as a nal irrigant may neutralize any residual sodium hypochlorite and oxygen and increase the bond strength of root canal sealers [19] and bonding systems to root dentin [20].…”

Section: Introductionmentioning

confidence: 99%

“…Due to its strong antioxidant properties, the use of EGCG as a nal irrigant may neutralize any residual sodium hypochlorite and oxygen and increase the bond strength of root canal sealers [19] and bonding systems to root dentin [20]. Recently, studies carried out by our research group developed a formulation based on EGCG for endodontic use that presented tissue compatibility and promoted the healing of periapical tissues [17], and the treatment of post radiotherapy teeth with EGCG inactivates the enzymatic activity of MMPs activated by radiotherapy [21]. Thus, it appears that in addition to playing an anti-in ammatory, antioxidant, antimicrobial and tissue regenerator role, intracanal medication with EGCG may inactivate MMPs.…”

Section: Introductionmentioning

confidence: 99%

Effect of epigallocatechin-3-gallate (EGCG) - based paste as intracanal dressing, in matrix metalloproteinases 2 and 9 in dog’s periapical lesions

Rossi

Pradela

Liévana

et al. 2022

Preprint

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Objectives: High expression of MMP-2 and MMP-9 in periapical lesions plays an important role in the degradation of the extracellular matrix. This study aimed to investigate the effect of epigallocatechin-3-gallate (EGCG)-based endodontic paste as an intracanal dressing on the expression of MMP-2 and MMP-9 in periapical lesions. Materials and Methodss: Periapical lesions were experimentally induced in 35 mature beagle dog premolars randomly divided into healthy teeth, untreated periapical lesions, periapical lesionstreated in a single session, and periapical lesions treated in two sessions with EGCG or calcium hydroxide-based pastes. The operator monitored the animals and performed euthanasia after 120 days for histopathologic and immunofluorescence analyses to assess the expression of MMP-2 and MMP-9. Then we perform the statistical analysis. Results: Endodontic treatment in two sessions using EGCG and calcium hydroxide-based pastes provided similar levels of repair of the apical and periapical tissues and neoformation of periodontal ligament fibers, cementum and alveolar bone. In both groups, the expression of MMP-2 and MMP-9 was minimal,and it was observed in the cytoplasm of fibroblasts, osteoblasts, cementoblasts, cementocytes and vascular endothelium. In healthy teeth, the expression of MMPs was minimal and was found in odontoblasts. Endodontic treatment in a single session did not result in full repair of periapical lesions, and they presented intense expression of MMP-2 and MMP-9, including in the cytoplasm of persistent inflammatory cells, similar to untreated lesions. In both groups treated in two sessions, the expression of MMP-2 and MMP-9 was similar to that in healthy teeth, and it was significantly lower than that in periapical lesions treated in a single session or untreated (p <0.001). Conclusions: The use of EGCG-based endodontic paste reduced the expression of MMP-2 and MMP-9 and allowed for the repair of periapical lesions, similar to calcium hydroxide-based paste, and it was superior to treatment performed in a single session. Clinical Relevance: This study was the first one that evaluated the use of EGCG- based paste as intracanal treatment resulted in a reduction of the expression of MMPs 2 and 9 and repair of periapical lesions, similarly to calcium hydroxide-based paste.

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Insights into the October 2021 Issue of the Journal of Endodontics

Aminoshariae

Azarpazhooh

Diogenes

et al. 2021

Journal of Endodontics

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An Epigallocatechin-3-gallate Formulation Developed for Endodontic Use: A Physicochemical and Biological Evaluation

Cited by 4 publications

References 40 publications

Effect of EGCG-based paste as intracanal dressing, in MMPs 2 and 9 expression in dog’s periapical lesions

Effect of EGCG-based paste as intracanal dressing, in MMPs 2 and 9 expression in dog’s periapical lesions

Effect of epigallocatechin-3-gallate (EGCG) - based paste as intracanal dressing, in matrix metalloproteinases 2 and 9 in dog’s periapical lesions

Insights into the October 2021 Issue of the Journal of Endodontics

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