An epidemiological study on ovine babesiosis in the Mashhad suburb area, province of Khorasan, Iran

Razmi, Gholam Reza; Naghibi, Abolghasem; Aslani, Mohammad Reza; Fathivand, M; Dastjerdi, Kazem

doi:10.1016/s0304-4017(02)00203-0

Cited by 35 publications

(35 citation statements)

References 8 publications

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“…In our study, no significant association was observed between the age of the animal and infection with B. ovis. The result is consistent with the findings of Razmi et al (2002Razmi et al ( , 2003, which reported that the differences between the Babesia spp. prevalence in all age groups and between male and female sheep were not statistically significant.…”

Section: Discussionsupporting

confidence: 83%

Determination of prevalence and risk factors for infection with Babesia ovis in small ruminants from Turkey by polymerase chain reaction

2006

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In this study, PCR and thin blood smear-based diagnostic methods were used to assess the frequency of Babesia infection in small ruminants. A total of 300 sheep and 100 goats from 37 randomly selected herds located in eight locations of eastern Turkey were examined for the presence of Babesia infection and any tick species on the body of the animals. Of 400 blood samples examined, 6 (1.5%) were positive for Babesia spp. piroplasms upon microscopic examination, whereas 33 (8.25%) were positive for the presence of B. ovis by PCR. The prevalence of babesiosis in small ruminants detected by PCR was significantly higher than obtained in microscopic examination of thin blood smears (P< 0.05). Thirty-three animals produced the DNA fragment specific for Babesia ovis of which 32 (10.66%) were sheep and 1 (1%) was goat. The difference between the prevalence of Babesia infection in sheep and goats were statistically significant (P < 0.05). The prevalence of Babesia infection in different age groups of sheep were statistically non-significant (P > 0.05). The frequency of B. ovis infection was higher in herds with tick burden than no tick burden (P < 0.05). Seven amplicons (six from sheep and one from goat) were sequenced. The resulting sequences were identical to the recently reported nucleotide sequence of B. ovis. A total of 510 ticks belonging to the Rhipicephalus spp. were collected from sheep. Ticks were identified to be R. bursa and R. turanicus on the basis of morphological features.

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Section: Discussionsupporting

confidence: 83%

Determination of prevalence and risk factors for infection with Babesia ovis in small ruminants from Turkey by polymerase chain reaction

2006

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“…Detection of Babesia ovis and B. motasi using semi nested-PCR method, revealed that B. ovis was more prevalent than B. motasi among sheep in Mazandaran and Golestan provinces. Our results confirm the findings of the study was conducted in Khorasan in north eastern Iran regarding distribution of B. ovis and B. motasi in the province (25). In different areas of Iran, B. ovis considered as a main causative agent of sheep babesiosis by molecular techniques.…”

Section: Discussionsupporting

confidence: 80%

“…A successful and healthy economy in livestock farming requires a serious management of livestock health, which is achievable through accurate and early diagnosis and therapy. In this study standard PCR of 18s rRNA showed Babesia and Theileria genera and semi nested-PCR detected B. ovis (3.6 %) and B. motasi (1.4 %) that had previously reported to occur in Iran (15,25,26). Standard PCR showed mixed infection with Babesia and Theileria in two sheep.…”

Section: Discussionmentioning

confidence: 87%

Molecular identification of ovine Babesia spp. in north of Iran

et al. 2013

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Background: Babesia is a blood-tissue parasite, which is transmitted by hard ticks from Ixodidae family. The parasite is the cause of babesiosis among small ruminants, cattle, human, dogs and other animals. Babesia is one of the main fatal factors among livestock in endemic regions such as Iran. The aim of this study was to identify Babesia spp infection using microscopic and molecular methods among small ruminants in Mazandaran and Golestan provinces, northern Iran, during 2011-2012. Materials and Methods:In this study, a total of 220 sheep and goats were selected from 22 flocks in different regions of these provinces and blood samples were taken from their ears. The samples were transferred to the laboratory. Then thick and thin smears were prepared, stained with Geimsa and examined under light microscope. Standard PCR and semi nested-PCR was performed to differentiate genus of Theileria and Babesia, also identify the species of Babesia. Results: From a total of 220 blood samples (160 sheep and 60 goats), 34 cases (15.4 %) showed Babesia infection using microscopic examination. Whereas, 11 cases (5%) were found positive for Babesia spp using standard PCR. Also, two positive cases were showed mixed infection with Theileria spp. In addition, two microscopic negative samples were positive by PCR assay.Using semi nested-PCR, Babesia ovis (n=10) and B. motasi (n=1) were detected. Conclusion: Our results shows ovine babesiosis is common in the Northern provinces of Iran. Moreover, Babesia ovis is the main causative agent of ovine babesiosis in northern Iran. The relatively high prevalence of Babesia infection in sheep and goats indicates the epizootic stability status of babesiosis in the northern part of Iran.

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“…Hoogstral and Wassef (1979;Hoogstral and Valdez 1980) studied ixodid ticks parasitizing wild sheep and goats in Iran focusing on maintaining natural foci of many hazardous diseases for human. Rahbari (1995) published ecological aspects of various species of ticks encountered on domestic animals in the northwest of Iran, and Razmi et al (2002) published a list of tick species of domestic animals in the northeast of the country.…”

Section: Introductionmentioning

confidence: 99%

Primary report on distribution of tick fauna in Iran

2007

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A tick survey was carried out in four different geographical areas of Iran, where the majority of the domestic ruminants in Iran exist. About 1,500 sheep, 1,200 goats and 500 cattle of 12 herds in different provinces lying in the corresponding zones were inspected for tick infestation. The occurrence of ticks on cattle, sheep and goats were 62, 55 and 57%, respectively, with no differences between the zones. The mean number of ticks on each animal was low (10-20 ticks per animal). Ixodid ticks were found throughout the year, whereas the soft tick Ornithodoros sp., which occurred in mountainous area with a significant difference in abundance, showed a clear pattern of seasonality, being generally present from November to March. The largest numbers of adult ixodid ticks were generally present from April to August. Rhipicephalus, Haemaphysalis and Dermacentor ticks occurred in the mountainous area, whereas Boophilus and Ixodes ticks were only present in the Caspian region. Hyalomma were very abundant in each zone but especially in the mountainous area, whereas Ixodes ticks were the minor genus.

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An epidemiological study on ovine babesiosis in the Mashhad suburb area, province of Khorasan, Iran

Cited by 35 publications

References 8 publications

Determination of prevalence and risk factors for infection with Babesia ovis in small ruminants from Turkey by polymerase chain reaction

Determination of prevalence and risk factors for infection with Babesia ovis in small ruminants from Turkey by polymerase chain reaction

Molecular identification of ovine Babesia spp. in north of Iran

Primary report on distribution of tick fauna in Iran

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