The aim is to assess the preventive measures of HCWs and determine the relationship between preventive measures and the demographic characteristics of HCWs. Methods: Cross-sectional study conducted among 500 HCWs in Wasit governorate hospitals. This sample was distributed throughout four hospitals include, AL-Zahra, AL-Karama teaching hospitals, AL-Kut gynecology hospital, and Martyr Fairouz general hospital, for the period of October 15th, 2020, to May 15th, 2021. Through self-report and questionnaires, data were collected and analysed through descriptive and inferential statistics. Results: Findings depict that majority of age groups among those who are 20-29 years old 63.2%, 50.2 were male residence in urban areas, 36.2 were medical assistance, and hold a diploma, work less than 10 years and within adequate training. Finding reveals that the majority of 49.8% were poor preventive measures. There was a significant relationship between preventive measures with age, and years of experience of HCWs. Conclusion: Poor preventive measures towards NIs, more years of experience, and more training the staff help raising professionals' health workers.