An epidemiological prospective of focal outbreak of dengue infection in Kathmandu, Nepal

Saud, Bhuvan; Adhikari, Saroj; Maharjan, Laxman; Paudel, Govinda; Amatya, Neetu; Amatya, Shristi

doi:10.1016/j.jcvp.2022.100063

Cited by 8 publications

(16 citation statements)

References 18 publications

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“…Males were slightly more affected than females (M: F=1.1:1) and similar for the admission. This finding was similar to the study done in India, Taiwan and Malaysia (7, 9-11). Most of the patients were from Pokhara as the hospital is in the heart of Pokhara.…”

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

“…Fever (96.5%) was the most common clinical symptom in this study and was the common finding in other studies too (7,9,(11)(12)(13)(14)(15)(16)(17)(18)(19)(20). The second most common symptom was headache (40.6%) which was similar to the findings of other studies (7,13). Proportion of patients with headache and myalgia were less in this study as compared to the studies which might be due to entry of the main symptom only during admission and ignoring the less troublesome symptoms by the patient in history taking (9,12,17,19,21).…”

Section: Discussionsupporting

confidence: 90%

“…Research on dengue has been limited in Nepal and only few papers are available. Limited research mainly focused on terai belts and mainly on serological and demographic characteristics (4)(5)(6)(7). This study focuses on all aspects including the laboratory parameters which will help to estimate the morbidity to a deeper extent which ultimately burdens the economical constraint of the individual, society and the country.…”

Section: Modes Of Transmission Are Variable Butmentioning

confidence: 99%

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Clinical and haematological profile of dengue among adult patients at a tertiary care hospital in Pokhara

Dhungana

Banstola

Banjara

2022

Preprint

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IntroductionDengue is an important infectious disease. This disease is prevalent in the terai belts of Nepal mainly. But in the last few years, the cases are in increasing trend in the hilly areas of Nepal also. Hence this study was done in an aim to study the clinical and haematological profile of the dengue cases.MethodsThis was a retrospective cross-sectional quantitative study done at a tertiary teaching hospital of Pokhara, Nepal after obtaining ethical approval from the institutional ethical committee. The data of serologically confirmed dengue cases, during the period of August 2019 to December 2019, of age above 15 years, were collected and analysed using SPSS 20. Descriptive analysis in terms of mean, median, percentage as well as t-test for nominal and chi-square tests were used to compare different parameters. P-value≤ 0.05 was considered statistically significant.ResultsOut of 922 patients, approximately one-half (50.5%) cases were seen during the month of September. Most (82.8%) were the inhabitants of Kaski district. Median age of presentation was 29 years with slightly more cases of males (52.4%). Three hundred and forty seven patients were admitted. Fever (96.5%) and headache (40.6%) were the most common symptoms on presentation of admitted cases. Leukopenia (55.3%) was more common than thrombocytopenia (47.6%) in the admitted cases. On comparison between admitted patients with warning signs and those without signs, no significant variation was seen in terms of age, total leukocyte count and total platelet count.ConclusionDengue is common in young population. Fever, headache and gastrointestinal symptoms are common among dengue patients. Leukopenia and thrombocytopenia are common laboratory features of dengue.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

Section: Modes Of Transmission Are Variable Butmentioning

confidence: 99%

See 2 more Smart Citations

Clinical and haematological profile of dengue among adult patients at a tertiary care hospital in Pokhara

Dhungana

Banstola

Banjara

2022

Preprint

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“…This finding was similar to the study done in Nepal, India, Taiwan and Malaysia. [7][8][9][10][11] The median age of presentation was 29 years. The older age group represented a minority (<5%) of cases.…”

Section: Discussionmentioning

confidence: 99%

Admitted Dengue Cases among the Adult Dengue Positive Cases in a Tertiary Care Centre: A Descriptive Cross-sectional Study

Dhungana

Banstola

Banjara³

2022

J Nepal Med Assoc

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Introduction: Dengue is an infectious disease. This disease is prevalent mainly in the terai belts of Nepal. But in the last few years, the cases are in increasing trend in the hilly areas of Nepal. The aim of this study was to find out the prevalence of admitted dengue cases among adult dengue-positive cases in a tertiary care centre. Methods: This was a descriptive cross-sectional study done at a tertiary teaching hospital after obtaining ethical approval from the Institutional Review Committee (Reference number: 063/2077/2078). Convenience sampling was used. The data of serologically confirmed dengue cases, during the period of 1 August 2019 to 1 December 2019, of ages above 15 years, were collected from the hospital records. Point estimate and 95% Confidence Interval were calculated. Results: Out of 922 adult dengue-positive patients, 347 (37.63%) (36.04-39.22, 95% Confidence Interval) were admitted. Among them, 154 (44.38%) cases were seen during the month of September. A total of 264 (76.08%) were the inhabitants of the Kaski district. A total of one hundred seventy eight (51.29%) cases were males. The most common symptoms seen were fever among 335 (96.54%) patients and headache among 141 (40.63%) patients. Leukopenia was seen in 192 (55.33%) patients and thrombocytopenia was seen in 165 (47.55%) of the admitted cases. Conclusions: The prevalence of admitted dengue cases was higher as compared to other studies done in similar settings.

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Molecular Investigation of DENV serotypes in the dengue outbreak of 2022 in Nepal

Katuwal,

Shrestha,

Ranjitkar

et al. 2023

Preprint

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Introduction Dengue, a viral infection highly prevalent in tropical regions, exhibits local variations in risk that are influenced by a combination of climatic, socioeconomic, and environmental factors. The disease is caused by four distinct yet closely related serotypes of the dengue virus: DENV-1, DENV-2, DENV-3, and DENV-4. Objectives The objective of this study is to identify the different serotypes of dengue virus responsible for the 2022 outbreak in Nepal, where dengue has been prevalent since 2006 but with limited availability of molecular information on the serotypes. Methodology Serum samples from suspected dengue patients visiting Dhulikhel hospital were analyzed using Dengue Ag and IgM/IgG Ab Kit (Biotrol Laboratories) to test the serum samples for the presence of IgG/IgM antibodies or NS1 Ag. The positive samples were stored at -80 ⁰C, and 89 samples were selected for further analysis. RNA was extracted from those positive samples using the Zymo Quick RNA Viral Kit, and RT-PCR was performed using the Sacace Dengue Real Genotype qPCR kit to identify the dengue virus serotypes present. Results The study included 89 samples, of which a higher percentage of sero-positivity was observed in females (52%) compared to males. Positive cases were distributed in 14 different districts, with the highest percentage (58.4%) in Kavre. Molecular analysis, of 53 out of 89 serologically positive samples, by qPCR revealed that DENV1 was the predominant serotype, followed by DEN3 (24.5%) and DENV2 (16.9%). DENV4 was not detected in any of the samples. The average Ct value of all serotypes was 17.6, with DENV3 having the lowest Ct value of 16.6, indicating a high viremia. Conclusion Our study, although limited in its coverage of Nepal, has provided molecular information on the serotypes responsible for the 2022 dengue outbreak. The high prevalence of DENV1 was observed, while DENV3 had a high viral load. This information provides valuable insight into the circulating serotypes of the virus in the region.

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An epidemiological prospective of focal outbreak of dengue infection in Kathmandu, Nepal

Cited by 8 publications

References 18 publications

Clinical and haematological profile of dengue among adult patients at a tertiary care hospital in Pokhara

Clinical and haematological profile of dengue among adult patients at a tertiary care hospital in Pokhara

Admitted Dengue Cases among the Adult Dengue Positive Cases in a Tertiary Care Centre: A Descriptive Cross-sectional Study

Molecular Investigation of DENV serotypes in the dengue outbreak of 2022 in Nepal

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