An epidemiological and ecological study of human alveolar echinococcosis transmission in south Gansu, China

Craig, Philip S.; Giraudoux, Patrick; Shi, D.; Bartholomot, B.; Barnish, G.; Delattre, P.; Quéré, Jean-Pierre; Harraga, S.; Bao, Guangjie; Wang, Y.; Lu, Fatemeh Taleb; Itō, Akira; Vuitton, Dominique A.

doi:10.1016/s0001-706x(00)00134-0

Cited by 172 publications

(166 citation statements)

References 22 publications

Supporting

Mentioning

156

Contrasting

Unclassified

Order By: Relevance

“…Additionally, tests based on the partially purified lipoproteins antigen B (AgB) and antigen 5 (both found in the germinal membrane, protoscolex parenchyma and cyst fluid [36]) are used either in ELISA or immunoblot assays. The major drawback of these tests is the lack of species specificity, as cross-reactions between AE and CE and many other helminthic infections are commonly observed (cestodes, 89%; nematodes, 39%; trematodes; 30%) [5,20,[37][38][39][40]. Assays based on a recombinant subunit of AgB, denoted rAgB8/2, showed sensitivities of 45-93% and specificities of 86-99% with the same cross-reactions as mentioned above [20,41,42].…”

Section: Discussionmentioning

confidence: 88%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

View full text Add to dashboard Cite

PURPOSE: Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. METHODS: The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. RESULTS: According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and >99.9% for the diagnosis of CE and AE, respectively. Antigens derived from E. multilocularis metacestodes had sensitivities ranging from 84 to 91% and >99.9% for the diagnosis of CE and AE, respectively. Specificities ranged from 92 to >99.9%. Post-test probabilities for the differential diagnosis of AE from liver neoplasias, CE from cystic liver lesions, and screening for AE in Switzerland were around 95, 86 and 2.2%, respectively. Cross-reactions with antibodies in sera of patients with other parasitic affections (fasciolosis, schistosomosis, amebosis, cysticercosis, and filarioses) did occur at variable frequencies, but could be eliminated through the use of confirmatory testing. CONCLUSIONS: Different metacestode-derived antigens of E. granulosus and E. multilocularis are valuable, widely accessible, and cost-efficient tools for the serological diagnosis of echinococcosis. However, confirmatory testing is necessary, due to the lack of species specificity and the occurrence of cross-reactions to other helminthic diseases. Abstract Purpose Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. Methods The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. Results According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and [99.9% for the diagnosis of CE and AE, respectively. Antigens derived from...

show abstract

Section: Discussionmentioning

confidence: 88%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

View full text Add to dashboard Cite

show abstract

“…An average of 120 subjects (range, 60-180 subjects) were examined per day, typically screened over the course of 8-12 * ass. ϭ associated with a positive serology using EmC, Em2, or both; Eg ϭ Echinococcus granulosus crude antigen (hydatid fluid); EmP ϭ Echinococcus multilocularis protoscolex purified antigen, according to Craig and others 6 ; Em2 ϭ Echinococcus multilocularis purified specific antigen, according to Gottstein and others 7 ; EmC ϭ Echinococcus multilocularis crude antigen.…”

Section: Resultsmentioning

confidence: 99%

Combined ultrasound and serologic screening for hepatic alveolar echinococcosis in central China.

Bartholomot¹,

Vuitton²,

Harraga³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

136

View full text Add to dashboard Cite

Abstract. Alveolar echinococcosis (AE), caused by Echinococcus multilocularis, is a zoonotic helminthic disease that can mimic malignancy. In the 1970s, foci of the disease were found in central China. The aim of the present study was to estimate the prevalence of AE in humans in 2 districts of south Gansu Province, China, by use of ultrasound and Echinococcus serology. After answering an epidemiological questionnaire, 2,482 volunteers from 28 villages underwent ultrasound. Serology via enzyme-linked immunosorbent assay for antibody activity was performed on whole blood collected on filter paper in all subjects; on serum from subjects with an abnormal ultrasound image; and on randomly chosen subjects that either had no lesions or had atypical lesions. At least one (25.3%) abnormal ultrasound image was observed in 630 of the subjects screened. A typical lesion of progressive AE was found in 84 subjects (3.4%). Serologies were positive in 77 (96%) of 80 of patients who had lesions typical of progressive AE. Ultrasound is useful for screening for AE in endemic regions.

show abstract

“…Epidemiological and ecological studies have been carried on since the 1980s in the Jura Mountains, France (Delattre, Pascal & Damange, 1985 ;Delattre et al 1988 ;Delattre, Giraudoux & Quere, 1990 ;Giraudoux, 1991 ;Bresson-Hadni et al 1994 ;Viel et al 1999 ;Raoul, 2001), and in Southern Gansu, China (Craig et al 1992 ;Giraudoux et al 1998 ;Courant et al 1999 ;Craig et al 2000). The present paper analyses and compares results obtained in both regions and suggests that at the regional scale landscape can affect human disease distribution through interaction with small mammal communities and their population dynamics.…”

Section: Introductionmentioning

confidence: 99%

Interactions between landscape changes and host communities can regulateEchinococcus multilocularistransmission

et al. 2003

Self Cite

View full text Add to dashboard Cite

An area close to the Qinghai-Tibet plateau region and subject to intensive deforestation contains a large focus of human alveolar echinococcosis while sporadic human cases occur in the Doubs region of eastern France. The current review analyses and compares epidemiological and ecological results obtained in both regions. Analysis of rodent species assemblages within quantified rural landscapes in central China and eastern France shows a significant association between host species for the pathogenic helminth Echinococcus multilocularis, with prevalences of human alveolar echinococcosis and with land area under shrubland or grassland. This suggests that at the regional scale landscape can affect human disease distribution through interaction with small mammal communities and their population dynamics. Lidicker's ROMPA hypothesis helps to explain this association and provides a novel explanation of how landscape changes may result in increased risk of a rodent-borne zoonotic disease.

show abstract

An epidemiological and ecological study of human alveolar echinococcosis transmission in south Gansu, China

Cited by 172 publications

References 22 publications

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

Combined ultrasound and serologic screening for hepatic alveolar echinococcosis in central China.

Interactions between landscape changes and host communities can regulateEchinococcus multilocularistransmission

Contact Info

Product

Resources

About