An eosinophil peroxidase activity assay accurately predicts eosinophilic chronic rhinosinusitis

Smith, Kristine A.; Gill, Amarbir S.; Pollard, Chelsea E.; Sumsion, Jorgen S; Saffari, Hedieh; Ashby, Shaelene; Witt, Benjamin L.; Shipman, Paige A.; Gabrielsen, David A.; Yim, Michael; Levy, Joshua M.; Oakley, Gretchen M.; Orlandi, Richard R.; Gleich, Gerald J.; Alt, Jeremiah A.; Pulsipher, Abigail

doi:10.1016/j.jaci.2023.04.012

Cited by 11 publications

(5 citation statements)

References 40 publications

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“…In recent years, a growing number of studies have confirmed that CRS subtypes classified according to JESREC score and components of JESREC score, including nasal polyps, peripheral eosinophilia, and lesion location, were closely related with olfactory function ( 14 , 21 – 23 ). Eosinophilia in peripheral blood and the nasal mucosa were common in CRS, especially in ECRS patients ( 24 ), and compared with CRS patients with normal olfactory function, patients with olfactory dysfunction had higher proportions of ECRS subtype and eosinophilia in our study, which was consistent with previous results ( 25 , 26 ). Ahn et al.…”

Section: Discussionsupporting

confidence: 92%

Association between peripheral eosinophilia, JESREC score, and olfactory dysfunction in patients with chronic rhinosinusitis

Zhang,

Li,

Wang

et al. 2024

Front. Immunol.

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ObjectiveThe purpose was to evaluate the relationship between peripheral eosinophilia, Japan Epidemiological Survey of Refractory Eosinophilic Chronic Rhinosinusitis (JESREC) score, and olfactory dysfunction in chronic rhinosinusitis (CRS) patients and to explore the accuracy and specific cut points of the JESREC score in predicting olfactory dysfunction.MethodsIn this cross-sectional, retrospective study, olfactory function was assessed by the Sniffin’ Sticks 12-item test and multivariate logistic regression analyses were carried out. Receiver operating characteristic curves were plotted to derive accuracy and cutoff values for the JESREC scores of the olfactory dysfunction criterion.ResultsA total of 354 patients [mean (SD) age, 50.0 (14.9) years; 41.8% women] were included in the final analysis. The prevalence of olfactory dysfunction was 46.3%. Individuals who had olfactory dysfunction were more likely to be male (64.6% vs. 52.6%), have eosinophilic chronic rhinosinusitis (ECRS) (39.0% vs. 7.9%), have a longer course of CRS (2.3 years vs. 1.5 years), have higher JESREC scores (8.5 vs. 4.5), and have higher proportions of nasal polyps (78.7% vs. 18.9%) and peripheral eosinophilia (3.3% vs. 1.4%). In logistic analysis, the percentage of eosinophils (1.25, 1.13–1.37), JESREC score (1.31, 1.22–1.40), bilateral lesion (2.06, 1.25–3.41), nasal polyps (15.83, 9.23–27.16), CT shadow (2.73, 1.69–4.43), and ECRS (6.86, 3.68–12.80) were associated with olfactory dysfunction in CRS patients after controlling for covariates, while peripheral neutrophils were not significant. In addition, the area under the curve was 0.778 and the cutoff value for JESREC score for olfactory dysfunction was defined as 5.5.ConclusionsPeripheral eosinophilia and high JESREC scores were significantly associated with the risk of olfactory dysfunction in CRS patients, and special attention should be paid to patients with a JESREC score ≥6.

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Section: Discussionsupporting

confidence: 92%

Association between peripheral eosinophilia, JESREC score, and olfactory dysfunction in patients with chronic rhinosinusitis

Zhang,

Li,

Wang

et al. 2024

Front. Immunol.

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Section: Discussionmentioning

confidence: 99%

“…The lack of a relationship between objective disease metrics and systemic markers of inflammation is common in CRS and continues to be a challenge in the search for sensitive and specific biomarkers for objective disease severity in CRS. 29,39 The study design/methodology demonstrates adherence to ethical guidelines, including obtaining informed consent and approval from the IRB, ensuring participant welfare and research integrity. Participants undergo thorough clinical evaluation, including medical history, physical examination, and imaging studies, enhancing the accuracy of CRS diagnosis and classification.…”

Section: Discussionmentioning

confidence: 99%

Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

Mahomva,

Smith,

Minkah

et al. 2024

JIR

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Background CCL19 has been shown to predict disease severity in COVID-19 and treatment response in rheumatoid arthritis. CCL19 can exert both pro- and anti-inflammatory effects and is elevated in chronic rhinosinusitis (CRS). However, its role in CRS remains unknown. This study sought to determine the transcriptional changes in CCL19, its receptors, and associated cytokines and their association with disease severity in CRS. Methods A clinical database of control subjects and patients with CRS was examined. Lund-Kennedy, Lund-Mackay, Sinonasal Outcomes Test 22 (SNOT-22), and rhinosinusitis disability index (RSDI) scores were collected at enrollment. mRNA was extracted from sinonasal tissues and subjected to multiplex gene expression analysis. Gene transcript differences between patients with CRS and controls were compared and correlated with disease severity metrics. Immunohistochemical analyses of CCL19, CCR7, and CCRL1 were conducted to compare differences in protein expression between cohorts. A subgroup analysis was performed to compare transcriptional and protein expression difference between patients with (CRSwNP) and without (CRSsNP) nasal polyps and controls. Results Thirty-eight subjects (control group, n=7; CRS group, n=31) were included in this study. CCRL1 ( p =0.0093) and CCR7 ( p =0.017) levels were significantly elevated in CRS compared to those in controls. CCL19 ( p =0.038) and CCR7 ( p =0.0097) levels were elevated in CRSwNP and CCRL1 was elevated in CRSsNP ( p =0.0004). CCR7 expression was significantly elevated in sinonasal epithelial cells in CRSwNP ( p =0.04). CCL19 expression was positively correlated with TNFA expression ( p <0.0002). CCL19 and CCR7 expression was positively correlated with SNOT-22 and RSDI scores ( p <0.05). Conclusion CCL19 and CCR7 may modulate TNF-α-driven pro-inflammatory signaling and contribute to increased disease severity in CRS. Mechanistic studies are required to further elucidate the role of CCRL1 in CRS.

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“…predicted ECRS through eosinophil peroxidase (EPX) analysis of ethmoid and nasal polyp tissue. 28 The study was invasive and unconventional. Thorwarth et al.…”

Section: Discussionmentioning

confidence: 99%

Predictive modeling for eosinophilic chronic rhinosinusitis: Nomogram and four machine learning approaches

Xiong,

Chen,

Zhang

et al. 2024

iScience

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An eosinophil peroxidase activity assay accurately predicts eosinophilic chronic rhinosinusitis

Cited by 11 publications

References 40 publications

Association between peripheral eosinophilia, JESREC score, and olfactory dysfunction in patients with chronic rhinosinusitis

Association between peripheral eosinophilia, JESREC score, and olfactory dysfunction in patients with chronic rhinosinusitis

Chemokine CCL19 and Its Receptors CCR7 and CCRL1 in Chronic Rhinosinusitis

Predictive modeling for eosinophilic chronic rhinosinusitis: Nomogram and four machine learning approaches

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