An enzyme-linked molecularly imprinted sorbent assay

Surugiu, Ioana; Ye, Lei; Yilmaz, Ecevit; Dzgoev, Anatoli; Danielsson, Bengt; Mosbach, Klaus; Haupt, Karsten

doi:10.1039/a908871g

Cited by 121 publications

(74 citation statements)

References 17 publications

(25 reference statements)

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“…Fifteen years since their discovery, however, aptamers have been obtained only for limited number of proteins because the production of the aptamers is difficult [1,2]. MIPs are the synthetic compounds that might be substituted for antibodies in immunoassay; however, the hydrophobic nature and highly cross-linked structure of the polymer limit the access of the large protein molecules to the binding sites of MIPs [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer

Zhang¹,

Shibata²,

Krawczyk³

et al. 2009

Talanta

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Facile and non-radioactive methods are desired for the sensitive detection and quantification of various proteins. Herein we describe a novel chemiluminescence (CL)-detection method of particular proteins based on direct binding of a dextran-luminol-biotin (DLB) CL polymer to the proteins on a poly(vinylidene difluoride) membrane. Among 32 kinds of the proteins screened, several proteins such as drug-metabolizing enzymes, cytochrome p450 (CYP)1A2, CYP2E1, and CYP3A4 had the ability to bind directly to the DLB polymer. The binding site in the polymer was owing to the framework of the modified dextran, which underwent oxidation and reduction procedures. This interaction might be the comprehensive effect of both electrostatic interaction and steric complementarities. CL intensity of the proteins detected by the polymer could be further enlarged by the mediation of avidin. The proposed CL-imaging method possesses potential as a rapid, facile, inexpensive and selective detection of the proteins.

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Section: Introductionmentioning

confidence: 99%

Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer

Zhang¹,

Shibata²,

Krawczyk³

et al. 2009

Talanta

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“…Antibodies can be difficult to generate against non-immunogenic molecule, they can be expensive and a reliance on animals in their production is viewed with some concern. As an alternative, this study uses molecular imprinting in order to develop an alternative analytical tool for use in the analysis of atrazine (Muldoon and Stanker, 1995;Bjarnason et al, 1999;Sergeyeva et al, 1999;Shoji et al, 2003;Matsui et al, 1997;Matsui et al, 2000) and pesticides in general (Surugiu et al, 2000(Surugiu et al, , 2001aHaupt, 2001;Pap et al, 2002;Cacho et al, 2004;Zhu et al, 2002;Bastide et al, 2005). These studies have shown that for atrazine-imprinted polymers the recognition process, and hence the selectivity of the polymer, relies on interactions between the polymer and the chlorine atom.…”

Section: Introductionmentioning

confidence: 99%

Concentration dependent atrazine–atrazine complex formation promotes selectivity in atrazine imprinted polymers

Lavignac

Brain

Allender

2006

Biosensors and Bioelectronics

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An atrazine (ATR) molecularly imprinted polymer (MIP) was prepared using a non-covalent strategy. The affinity and selectivity of the polymer was initially evaluated under non-equilibrium conditions and the polymer was shown to possess good template selectivity. The selectivity of the polymer was further investigated under equilibrium conditions and over a range of concentrations using Scatchard plots and Hill plots and by assessing distribution coefficients and normalised selectivity values. It was observed that both selectivity and affinity were dependent on the concentration of the ligand and that unusually selectivity and affinity were better at higher atrazine concentrations. It was concluded that this phenomenon resulted from the formation of atrazine-atrazine complexes during the pre-polymerisation stage and during rebinding and that the polymer demonstrated improved atrazine affinity when the conditions favoured complex formation.

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“…Both terms emphasize the similarity between antigen/antibody affinity and the attraction of a template for its imprinted polymer. MIPs and antibodies can be used in competitive binding assays [42] and incorporated in sensors and membranes [33]. However, important differences exist between MIPs and antibodies.…”

Section: Molecularly Imprinted Polymers Compared To Immunosorbentsmentioning

confidence: 99%

Ultraselective Sorbents. Task 2: Molecularly Imprinted Polymers (MIPs)/Stabilized Antibody Fragments (STABs). Final Report -- Fiscal Year (FY) 2005

Harvey¹

2005

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EXECUTIVE SUMMARYTrace analysis of signature compounds is usually accomplished by concentrating the target signature compound from a large volume environmental sample on an appropriate sorbent. Unfortunately, the organic matrix components also become concentrated, which necessitates separating the signature compound from this complex mixture in sufficient purity to allow identification and quantification. Due to the complexity of the mixture, sophisticated, laboratory-based multidimensional instrumentation often is required to provide adequate separation.The goal of this research is to develop highly selective sorbents that will enable collection of relatively pure analyte fractions during the sampling step. Due to the high purity of the initial fraction, subsequent analytical steps leading to high-integrity identifications at trace concentrations can be greatly simplified. The required analytical instrumentation can reflect this simplification by being made more compact, lightweight, and field portable. This advancement is made possible by the high degree of matrix discrimination accomplished during the sampling step.Two different selective sorbents were developed and evaluated in this research. Both sorbents must be capable of operation in nonaqueous environments to protect hydrolytically sensitive analytes during analysis. The approaches investigated were nonaqueous immunochromatography featuring the use of stabilized antibody fragments (STABs) and molecularly imprinted polymers (MIPs). Both approaches were pursued in Fiscal Year (FY) 2003, during the course of which it became clear that the MIPs approach had clear advantages over STABs. Further research efforts during FY 2004 and beyond focused exclusively on the development of MIPs.Our MIP studies have had two distinct thrusts: one thrust targeted volatile organic signatures, and the other thrust targeted nonvolatile phosphate or methylphosphonate half-acid esters. The first studies targeted the volatile signatures. By nature these volatile compounds do not contain polar functionalities in their chemical structures. Since most imprinting is based on polar functional group interactions, these compounds proved difficult to imprint. Nonetheless, we were successful in making MIPs that targeted tributyl phosphate (TBP) and diisopropyl methylphosphonate (DIMP). Selective interactions were not pronounced, as expected due to the weak interactions; however, these sorbents were capable of impressive selective capture of the target analyte with near-quantitative recovery from extremely complex environmental samples. These results were summarized in the manuscript submitted to the Journal of Separation Science. During the publication review process, we were asked to provide additional experiments. The experiments demonstrated that 1) our MIP sorbents had the expected cross reactivity toward structurally related analytes and, 2) our MIP selectivity was superior to traditional normal-phase sorbents, e.g., silica or alumina. We also calculated typical matrix discriminatio...

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An enzyme-linked molecularly imprinted sorbent assay

Cited by 121 publications

References 17 publications

Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer

Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol–biotin chemiluminescent polymer

Concentration dependent atrazine–atrazine complex formation promotes selectivity in atrazine imprinted polymers

Ultraselective Sorbents. Task 2: Molecularly Imprinted Polymers (MIPs)/Stabilized Antibody Fragments (STABs). Final Report -- Fiscal Year (FY) 2005

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