An enzyme-linked immunosorbent assay for blood-group A and B enzymes

Yazawa, Shin; Nakajima, Tamiko; Kameyama, Noriaki; Saga, Koh-Ichi; Tachikawa, Tetsuya

doi:10.1016/0008-6215(93)84232-u

Cited by 5 publications

(2 citation statements)

References 20 publications

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“…<2> [18]) [18] P UDP + N-acetyl-a-d-galactosaminyl-1,3-[asialo-porcine submaxillary mucin of A-negative blood-type] S UDP-N-acetyl-d-galactosamine + glycoprotein a-l-fucosyl-1,2-d-galactose <1, 2, 9, 10> (Reversibility: ? <1, 2, 9, 10> [12,13,14,15,16,17,18,19]) [12,13,14,15,16,17,18,19] [14,15,16,17,18,19] S UDP-N-acetyl-d-galactosamine + lacto-N-fucopentaose I <1> (<1> i.e. Fuca(1-2)Galb(1-3)GlcNAcb(1-3)Galb(1-4)Glc [14,15,19]) [14,15,19]…”

Section: Substrates and Productsmentioning

confidence: 99%

“…2 Source Organism <1> Homo sapiens (women [19]; blood type A or AB [19]; blood group A1 [17]; blood group A [14,15,16]; only four amino acids are responsible for B and A blood group enzymes R176G, G235S, L266M and G268A, the latter two amino acids are responsible for the difference in donor specificity and the first two have roles in acceptor binding and turnover [1,8,9]; gastric cancer tissue of type 0 individuals, 3 of 31 show A-antigen production [11]; blood group A1 and A2 [12]) [1,3,6,7,8,9,10,11,12,13,14,15,16,17,19] <2> Sus scrofa [18] <3> Rattus norvegicus [2] <4> Mus musculus domesticus (strain BALB/c, has a cis-AB gene that encodes an enzyme with both A and B transferase activities [4]) [4] <5> Mus musculus breviostris (strain BFM/2Msf, has a cis-AB gene that encodes an enzyme with both A and B transferase activities [4]) [4] <6> Mus musculus castaneus (strain CAST/Ei and HMI/Msf, has a cis-AB gene that encodes an enzyme with both A and B transferase activities [4]) [4] <7> Mus spicilegus (strain ZBN, has a cis-AB gene that encodes an enzyme with both A and B transferase activities [4]) [4] <8> Mesocricetus auratus (Syrian hamster [5]) [5] <9> Macaca fascicularis (crab-eating monkey [12])…”

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Glycoprotein-fucosylgalactoside α-N-acetylgalactosaminyltransferase

Springer Handbook of Enzymes

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Glycoprotein-fucosylgalactoside a-N-acetylgalactosaminyltransferase 2.4.1.40 1 Nomenclature EC number 2.4.1.40 Systematic name UDP-N-acetyl-d-galactosamine:glycoprotein-a-l-fucosyl-(1,2)-d-galactose 3-N-acetyl-d-galactosaminyltransferase Recommended name glycoprotein-fucosylgalactoside a-N-acetylgalactosaminyltransferase Synonyms A-transferase GTA <1> [1, 3, 9] UDP-GalNAc:Fuca1-2Gala1-3-N-acetylgalactosaminyltransferase UDP-N-acetyl-d-galactosamine:a-l-fucosyl-1,2-d-galactose 3-N-acetyl-d-galactosaminyltransferase a-3-N-acetylgalactosaminyltransferase blood-group substance A-dependent acetylgalactosaminyltransferase blood-group substance a-acetyltransferase fucosylgalactose acetylgalactosaminyltransferase histo-blood group A acetylgalactosaminyltransferase histo-blood group A glycosyltransferase (Fuca1-2Gala1-3-N-acetylgalactosaminyltransferase) histo-blood group A transferase

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Section: Substrates and Productsmentioning

confidence: 99%

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confidence: 99%

Glycoprotein-fucosylgalactoside α-N-acetylgalactosaminyltransferase

Springer Handbook of Enzymes

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α2→3Sialyltransferase associated with the synthesis of CA 19-9 in colorectal tumors

Akamatsu

Yazawa

Tachikawa

et al. 1996

Cancer

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BACKGROUND A novel assay method specific for α2→3sialyltransferase that seems to be responsible for the synthesis of CA 19‐9 antigen was developed and the levels of the enzyme in colorectal tumor tissues were measured and compared with the levels of α1→4fucosyltransferase and the CA 19‐9 antigen. METHODS Lacto‐N‐biose I (Galβ1→3GlcNAcβ, Lewisc [Lec]) and 6‐O‐methyl‐Lec(Galβ1→3[6OMe]GlcNAcβ) were synthesized and covalently attached to bovine serum albumin (BSA). These two substrates were incubated with extracts from colorectal tissues in the presence of cytidine 5′‐monophospho‐N‐acetylneuraminic acid (CMP‐NeuAc) and their resulting products were detected by a sequential use of anti‐BSA monoclonal antibody‐coated beads and 125I‐labelled anti‐sialylated Lec antibody. Levels of α2→3sialyltransferase and α1→4fucosyltransferase activities and CA 19‐9 antigen were measured in the extracts from colorectal tumors and their adjacent normal tissues. RESULTS 6‐O‐Methyl‐Lec‐BSA showed a strong acceptor activity compared with Lec‐BSA and was used as a specific acceptor for α2→3sialyltransferase. Similar elevation patterns of α2→3sialyltransferase activities and CA 19‐9 antigen levels were observed in tumor extracts but no clear correlation was present between the level of α1→4fucosyltransferase activities and CA 19‐9 antigen levels in the same extracts. CONCLUSIONS The accumulation of CA 19‐9 antigen in colorectal tumors might be caused mainly by the activation of α2→3sialyltransferase but not by that of α1→4fucosyltransferase. Cancer 1996;77:1694‐700.

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Fucosylgalactoside 3-α-galactosyltransferase

Springer Handbook of Enzymes

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An enzyme-linked immunosorbent assay for blood-group A and B enzymes

Cited by 5 publications

References 20 publications

Glycoprotein-fucosylgalactoside α-N-acetylgalactosaminyltransferase

Glycoprotein-fucosylgalactoside α-N-acetylgalactosaminyltransferase

α2→3Sialyltransferase associated with the synthesis of CA 19-9 in colorectal tumors

Fucosylgalactoside 3-α-galactosyltransferase

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