An enzyme-linked immunosorbent assay (ELISA) for urinary free cortisol

Lewis, John G.; Manley, Laurette; Jc, Townsend; Pa, Elder

doi:10.1016/0009-8981(86)90053-7

Cited by 24 publications

(9 citation statements)

References 3 publications

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“…If the 24 h urinary cortisol excretion (reference range: 100–400 nmol) was elevated, a 1‐mg dexamethasone suppression test (reference range < 50 nmol/L) was performed. Serum cortisol and 24 h urine cortisol excretion were measured by in‐house ELISA method (Canterbury Health Laboratory) . Patients with both an elevated 24 h urine cortisol and post 1 mg‐dexamethasone cortisol underwent clinical review by an Endocrinologist.…”

Section: Methodsmentioning

confidence: 99%

“…Serum cortisol and 24 h urine cortisol excretion were measured by in-house ELISA method (Canterbury Health Laboratory). 9,10 Patients with both an elevated 24 h urine cortisol and post 1 mg-dexamethasone cortisol underwent clinical review by an Endocrinologist. Patients were diagnosed with subclinical Cushing syndrome (SCS), as per the commonly used definition in the literature at the time, that is, the absence of clinical signs of cortisol excess plus at least two biochemical abnormalities of the hypothalamic-pituitary-adrenal axis.…”

Section: Biochemical Analysismentioning

confidence: 99%

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Characteristics of adrenal incidentalomas in a New Zealand centre

Goh

Phillips

Hunt

et al. 2018

Internal Medicine Journal

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Section: Methodsmentioning

confidence: 99%

Section: Biochemical Analysismentioning

confidence: 99%

Characteristics of adrenal incidentalomas in a New Zealand centre

Goh

Phillips

Hunt

et al. 2018

Internal Medicine Journal

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“…AVP (Sadler, et al, 1983) and CRH (Ellis e f al., 1990). Plasma cortisol was measured by enzymelinked radiosorbent assay (Lewis et al, 1986). Plasma adrenaline and noradrenaline were measured by high performance liquid chromatography with electrochemical detection after extraction with alumina (Peuler &Johnson, 1977).…”

Section: Assay Methodsmentioning

confidence: 99%

The plasma ACTH, AVP, CRH and catecholamine responses to conventional and laparoscopic cholecystectomy

Donald

Perry

Wittert

et al. 1993

Clinical Endocrinology

105

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For both procedures, the peak of ACTH secretion after incision is likely to be AVP dependent, and the timing of peak levels of these two hormones was significantly related. Subsequent ACTH secretion may be the result of an interaction between AVP and CRH. Laparoscopic cholecystectomy results in a smaller AVP rise than does the conventional procedure, and plasma AVP falls more rapidly post-operatively. During the period of observation, ACTH, CRH, cortisol and adrenaline responses were not significantly lessened by the laparoscopic approach, but there was a significant increase in the noradrenaline response. Stress hormone monitoring may assist further improvements in surgical technique.

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“…If the 24‐h urinary cortisol excretion (reference range: 100–400 nmol) was elevated, a 1‐mg overnight dexamethasone suppression test (reference range < 50 nmol/L) was performed. Serum cortisol and urine cortisol excretion were measured by in‐house ELISA method (Canterbury Health Laboratory) . Patients with both an elevated 24‐h urine cortisol and abnormal post 1‐mg dexamethasone cortisol underwent clinical review by an endocrinologist, plus additional hormonal investigations including ACTH and DHEA‐S if appropriate.…”

Section: Methodsmentioning

confidence: 99%

“…Serum cortisol and urine cortisol excretion were measured by in-house ELISA method (Canterbury Health Laboratory). 13,14 Patients with both an elevated 24-h urine cortisol and abnormal post 1-mg dexamethasone cortisol underwent clinical review by an endocrinologist, plus additional hormonal investigations including ACTH and DHEA-S if appropriate. Patients were diagnosed with subclinical Cushing syndrome (SCS), as per the commonly used definition in the literature at the time, that is, the absence of clinical signs of cortisol excess plus at least two biochemical abnormalities of the hypothalamic-pituitary-adrenal axis.…”

Section: Biochemical Analysismentioning

confidence: 99%

Three‐year follow up of adrenal incidentalomas in a New Zealand centre

Goh

Phillips

Hunt

et al. 2020

Internal Medicine Journal

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Background The international guidelines for management of adrenal incidentalomas (AI) are becoming more conservative. These changes are based on the growing body of evidence suggesting that non‐functioning adenomas have a low likelihood of becoming functional or malignant over time. Aims To follow up at least 100 patients for 3 years who were originally found to have benign adrenal adenomas which were non‐functional or had subclinical Cushing syndrome (SCS). Methods This study prospectively evaluated consecutive patients aged 18 years or older with benign adrenal incidentalomas (AI), not treated with adrenalectomy, which were non‐functioning or had SCS. The initial and follow‐up evaluation, including clinical assessment, hormonal investigations and imaging were coordinated via a standardised nurse‐led AI clinic. Results Of 233 patients referred to the AI clinic, 101 patients met the inclusion criteria and completed 3‐year follow up. Most of those excluded were due to incomplete initial or follow‐up evaluation or were not true AI. Most AI either remained stable or decreased in size on repeat imaging, while 5% of patients had AI enlargement of >5 mm diameter. No patient developed features suggesting adrenal carcinoma. Ninety‐two patients had an initial diagnosis of non‐functioning adenoma and nine patients had SCS. After 3 years (range 2.9–4.7 years), five of the nine patients with SCS showed normalisation of cortisol parameters (44%), and five of the 92 non‐functional AI patients developed SCS (5%). Conclusion After 3 years of follow up, approximately half of patients with SCS normalised, while 5% of patients with initially non‐functioning adenomas developed biochemical evidence of SCS. This study found a low likelihood of progressive hormonal excess with no evidence of malignancy developing on follow‐up evaluation, providing support for the shift towards the more conservative approach to management of AI recommended in recent guidelines.

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An enzyme-linked immunosorbent assay (ELISA) for urinary free cortisol

Cited by 24 publications

References 3 publications

Characteristics of adrenal incidentalomas in a New Zealand centre

Characteristics of adrenal incidentalomas in a New Zealand centre

The plasma ACTH, AVP, CRH and catecholamine responses to conventional and laparoscopic cholecystectomy

Three‐year follow up of adrenal incidentalomas in a New Zealand centre

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