An enzyme-linked immunoassay for circulating immune complexes using solid phased goat C1q

Lin, Tsue-Ming; Halbert, Seymour P.; Cort, Robert; Blaschke, Mary Jean

doi:10.1016/0022-1759(83)90423-4

Cited by 8 publications

(2 citation statements)

References 26 publications

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“…Lin et al [17] used 2 different methods for the purification of goat C1q: in one, the procedure was based on two successive precipitation steps at low ionic strength, and followed by an additional purification through a Sepharose CL-6B gel filtration column; and in the other, they used a two-step chromatography, with a BioRex 70 and a Sepharose CL-6B columns. C1q is a protein which has a high affinity for heparin and IgG, so McKay [40] used these characteristics to make a two-step affinity chromatography procedure to purify C1q from various species (human, rat, rabbit, dog and sheep).…”

Section: Discussionmentioning

confidence: 99%

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The complement system of the goat: Haemolytic assays and isolation of major proteins

et al. 2012

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BackgroundThe aim of the present study was to develop a haemolytic assay for the study of the complement system in dairy goats (Capra aegagrus hircus) and to characterize the major goat complement system proteins.ResultsThe commonly used sheep erythrocyte sensitized with rabbit antibodies were not sensitive to lysis by goat serum, but the combination of human red blood cells (RBC) plus rabbit antibodies was the best option found for goat complement assay. A buffer based on HEPES instead of the classical veronal (barbitone) was developed. Three proteins were isolated: factor H, C1q and C3 and these were compared with the corresponding human proteins. A novel affinity chromatography technique was developed for isolation of factor H.ConclusionsHuman RBC plus rabbit antibodies were a suitable option for haemolytic assays. The isolated proteins are similar to the human counterparts.

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Section: Discussionmentioning

confidence: 99%

“…Other published work on goat complement includes studies of infection with some parasites like Trypanosoma evans i [15] or with the estimation of the molecular size of the C1 complex [16], purification of C1q [17] or mapping of MHC-linked complement genes [18]. In theses studies, C2 and C4 were found to be MHC-linked, as in humans.…”

Section: Introductionmentioning

confidence: 99%