“…By examining hCG levels, we can also understand the treatment effect on these diseases. Recently, a large number of analytical methods have been reported for hCG detection, such as electrochemical methods, 5–7 colorimetric immunoassays, 8–10 fluorescence methods, 11,12 enzyme immunoassays, 13,14 etc . It is worth mentioning that the enzyme-linked immunosorbent assay (ELISA), which is an immunoassay technology developed on the basis of immune-enzymatic techniques, has become a pioneering topic in the field of analytical chemistry.…”