An enzyme immunoassay for the detection of all Salmonella using a combination of a myeloma protein and a hybridoma antibody

Mattingly, Jerome A.

doi:10.1016/0022-1759(84)90040-1

Cited by 42 publications

(20 citation statements)

References 19 publications

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“…The nature of the probe antibody used and time of sample collection probably explained the variations in the sensitivity and specificity reported. More recently, there have been reports of monoclonal antibodies directed against different Salmonella antigens, such as 09 [16,17], Vi [23,24], and barber protein Bp [21], and of antibodies that bind to flagellar determinants of the organism but that are not directed toward the H antigen [19,20,22]. The anti-Vi MAbs [24] cross-react only with S. dublin and Citrobacter, the anti-09 MAbs [16,17] cross-react with all group D Salmonellae, S. panama, and S. enteritidis, and the anti-Bp MAbs [21] cross-react with S. paratyphi C, S. choleraesuis, and S. typhimurium.…”

Section: Discussionmentioning

confidence: 99%

“…The anti-Vi MAbs [24] cross-react only with S. dublin and Citrobacter, the anti-09 MAbs [16,17] cross-react with all group D Salmonellae, S. panama, and S. enteritidis, and the anti-Bp MAbs [21] cross-react with S. paratyphi C, S. choleraesuis, and S. typhimurium. Finally, the two antibodies that bind to flagellar determinants [19,20,22] were reported to detect all Salmonella organisms in food and stool samples.…”

Section: Discussionmentioning

confidence: 99%

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Production of Specific Monoclonal Antibodies to Salmonella typhi Flagellin and Possible Application to Immunodiagnosis of Typhoid Fever

Sadallah¹,

Brighouse²,

Giudice³

et al. 1990

Journal of Infectious Diseases

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Four murine monoclonal antibodies (MAbs)to Salmonella typhi flagellin wereproduced. These MAbsdid not react with eight other enterobacterialstrains tested: Salmonella enteritidis, Salmonella typhimurium, Salmonella paratyphi A, Escherichia coli, Shigella flexneri, Shigella sonnei, Yersinia enterocolitica, and Campylobacter jejuni. Allfour MAbscross-reactedwith Salmonella muenchen flagellin indicating specificityfor d antigenic flagellar epitope. One MAb (C4) was selected to develop a double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect S. typhi flagellin in serum samples. By use of this assay S. typhi flagellar antigen was detected in 95.5% of serum samples from patients with positive hemoculture for S. typhi, in 93.6% of samples from patients with positive serodiagnosis of typhoid fever, in 26% of samples collected from patients who were initially hemoculture-positive for S. typhi and who had undergone 7-8 d of chemotherapy, in 8.5% of samples from healthy persons from an endemic area, and in no samples from healthy persons from a nonendemic area. The presence of high levelsof flagellin antibody titers did not interfere with the antigen detection. The detection of S. typhi flagellar antigen in patient serum may have practical value for rapid diagnosis of typhoid fever.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Production of Specific Monoclonal Antibodies to Salmonella typhi Flagellin and Possible Application to Immunodiagnosis of Typhoid Fever

Sadallah¹,

Brighouse²,

Giudice³

et al. 1990

Journal of Infectious Diseases

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“…Further, this pathogen is often present in very low numbers among a background of indigenous microflora, thus rendering recovery of target organism difficult (Swaminathan and Feng 1994). Enzyme linked assays using polyvalent, polyclonal antibodies or monoclonal antibodies against somatic or flagellar antigens (Krysinski and Heimsch 1977;Mattingly 1984) and DNA hybridization assays using polynucleotide or oligonucleotide probes (Aabo et al 1993;Bej et al 1994) have also been developed to detect this pathogenic microorganism. However, these assays have a problem of cross-reactivity, which produce false positive results (Mansfield and Forsythe 2000;Walker et al 2001).…”

Section: Introductionmentioning

confidence: 98%

Rapid detection of Salmonella enterica serovars by multiplex PCR

Jeyasekaran

Raj

Shakila

et al. 2010

World J Microbiol Biotechnol

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A multiplex PCR based assay was developed for the identification of the genus Salmonella. Five sets of primers from different genomic sequences such as fimA, himA, hns, invA and hto genes were selected for the identification of serogroups of Salmonella enterica such as S. Typhi, S. ParatyphiA, S. Typhimurium, S. Enteritidis and S. Weltevreden. The selected primers amplified products with the sizes of 85, 123, 152, 275 and 496 bp, respectively, for the genus Salmonella. This assay was found to be highly sensitive, as it could detect 5 cells of Salmonella and 1,000 fg of genomic DNA. Amplification of DNA extracted from other genera viz. V. cholerae and E. coli yielded negative results. This assay provides specific and reliable results and allows for the cost-effective detection of Salmonella in one reaction tube in mixed bacterial communities.

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“…Entre essas técnicas, destacam-se as imunoenzimáticas que empregam anticorpos marcados com uma enzima cromogênica [27]. As técni-Detecção de Salmonella em alimentos, Reis et al cas imunoenzimáticas podem empregar anticorpos policlonais e também monoclonais [20,21,22].…”

Section: -Introduçãounclassified

Padronização de um teste imunoenzimático para detecção de Salmonella em alimentos

Reis

Mamizuka

Franco

2002

Ciênc. Tecnol. Aliment.

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RESUMOA metodologia convencional utilizada para detecção de Salmonella em alimentos é trabalhosa, apresenta custo elevado e os resultados definitivos somente estão disponíveis após 96 horas. Vários métodos rápidos têm sido propostos, sendo os testes imunoenzimáticos os mais empregados. Este estudo relata o desenvolvimento de um teste imunoenzimático para detecção de Salmonella em alimentos, empregando-se um anti-soro policlonal monovalente contendo aglutininas f,g,s, não absorvido, e um anti-soro polivalente absorvido contendo as aglutininas e,h; 1,6; i; 1,2; f,g,s e m,t. A eficiência foi comparada com a da metodologia de cultivo tradicional. O teste imunoenzimático foi empregado para a detecção de Salmonella em amostras de alimentos infantis experimentalmente inoculadas com este patógeno e com outras enterobactérias, em diferentes proporções. O teste imunoenzimático revelou-se significativamente mais sensível que o método de cultivo. Esse mesmo teste, utilizando-se o anti-soro f,g,s não absorvido com antígenos heterólogos revelou concordância de 89,6% com o método de cultivo e sensibilidade de 100,0%. Por outro lado, empregando-se o anti-soro polivalente absorvido, a concordância com o método de cultivo foi de 81,3% embora a sensibilidade tenha se mantido no mesmo nível. O desempenho do teste imunoenzimático empregando-se um desses dois anti-soros indica um grande potencial de aplicação como método de triagem na detecção de Salmonella em alimentos. Palavras-chave: detecção; Salmonella; teste imunoenzimático; alimentos. SUMMARYSTANDARDIZATION OF AN ENZYME IMMUNOASSAY FOR DETECTION OF Salmonella IN FOODS. The conventional method for detection of Salmonella in foods is cumbersome, it is not cost-effective and results are available only after 96h. Many alternative rapid methods have been already proposed and enzyme immunoassays are the most common. This study reports the standardization of a new enzyme immunoassay for detection of Salmonella in foods, based on a policlonal non-absorbed antiserum containing f,g,s aglutinins and a pool of policlonal absorbed antisera, containing e,h; 1,6; i, 1,2, f,g,s and m,t aglutinins. The efficiency of the new assay was compared to that of the conventional method. The immunoassay was used for detection of Salmonella in baby-foods experimentally contaminated with different proportions of Salmonella and other enterobacteria. The sensitivity of the new assay was significantly higher than that of the conventional method. The agreement between the assay using the non-absorbed f,g,s antiserum and the conventional method was 89.6% and the sensitivity was 100.0%. When the pool of absorbed antisera was used, the agreement between results was 81.3% and the sensitivity 100.0%. Results indicate that this immunoassay has a good potential to be used as an alternative method for screening Salmonella in foods. Keywords: detection; Salmonella ; enzyme immunoassay; foods. PADRONIZAÇÃO DE UM TESTE IMUNOENZIMÁTICO PARA DETECÇÃO DE Salmonella EM ALIMENTOS 1Regina Baptista dos REIS 2, *, Elsa M. MAMIZ...

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An enzyme immunoassay for the detection of all Salmonella using a combination of a myeloma protein and a hybridoma antibody

Cited by 42 publications

References 19 publications

Production of Specific Monoclonal Antibodies to Salmonella typhi Flagellin and Possible Application to Immunodiagnosis of Typhoid Fever

Production of Specific Monoclonal Antibodies to Salmonella typhi Flagellin and Possible Application to Immunodiagnosis of Typhoid Fever

Rapid detection of Salmonella enterica serovars by multiplex PCR

Padronização de um teste imunoenzimático para detecção de Salmonella em alimentos

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