An enzyme immunoassay for the environmental monitoring of the herbicide bromacil

Bekheit, Hassan K. M.; Lucas, Anne D.; Szurdoki, Ferenc; Gee, Shirley J.; Hammock, Bruce D.

doi:10.1021/jf00035a075

Cited by 35 publications

(25 citation statements)

References 29 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Bromacil is used to protect citrus and pineapple and is also applied as a general weed killer on noncrop land. Immunoassays in various formats were devised for the analysis of this herbicide (Szurdoki et al, 1992(Szurdoki et al, , 1995cBekheit et al, 1993;Wengatz et al, 1995). Li etal., 1991;Harris et al 1995aMarco et al, 1993aSzèkacs et al, 1995Schneider and Hammock, 1992Schneider et al, 1994Karu etal., 1994Harris et al, 1995bSzurdoki et al, 1992Wengatz et al, 1995Marco et al, 1993b Szurdoki et al, 1995 * P: polyclonal; M: monoclonal.…”

Section: Resultsmentioning

confidence: 99%

Rapid assays for environmental and biological monitoring

Szurdoki¹,

Jaeger²,

Harris³

et al. 1996

Journal of Environmental Science and Health, Part B

View full text Add to dashboard Cite

Rapid, inexpensive, sensitive, and selective enzyme-linked immunosorbent assays (ELISAs) now are utilized in environmental science. In this laboratory, many ELISAs have been developed for pesticides and other toxic substances and also for their metabolites. Compounds for which ELISAs have recently been devised include insecticides (organophosphates, carbaryl, pyrethroids, and fenoxycarb), herbicides (s-triazines, arylureas, triclopyr, and bromacil), fungicides (myclobutanil), TCDD, and metabolites of naphthalene and toluene. New rapid assays have been developed for mercury.

show abstract

Section: Resultsmentioning

confidence: 99%

Rapid assays for environmental and biological monitoring

Szurdoki¹,

Jaeger²,

Harris³

et al. 1996

Journal of Environmental Science and Health, Part B

View full text Add to dashboard Cite

show abstract

“…This assay for bromacil is a modified version of a previously reported assay (Bekheit et al 1993). Ninety-six well microtiter plates (Nunc Maxisorp immuno-plates, Roskilde, Denmark) were coated with 100 µL of 0.35-µg/mL coating antigen 2b-CONA (Conalbumin; Bekheit et al 1993; Fig 1) in carbonate-bicarbonate buffer (pH 9.6).…”

Section: Methodsmentioning

confidence: 99%

“…Ninety-six well microtiter plates (Nunc Maxisorp immuno-plates, Roskilde, Denmark) were coated with 100 µL of 0.35-µg/mL coating antigen 2b-CONA (Conalbumin; Bekheit et al 1993; Fig 1) in carbonate-bicarbonate buffer (pH 9.6). The plates were sealed with an acetate plate sealer and incubated overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Regulatory Application of ELISA: Compliance Monitoring of Bromacil in Soil

Linde

Gee

Goh

et al. 1996

Bulletin of Environmental Contamination and Toxicology

View full text Add to dashboard Cite

“…Intermediate 4-AP was obtained using the efficient reagent combination of N-hydroxysulfosuccinimide (sulfo-NHS) and water soluble carbodiimide (EDC, Figure 2) (32).Assay Procedures. The assays were performed according to the usual solid phase immunoassay methods, the protocols were similar to those routinely used in our laboratory(33,34). Features specific to the mercury assays are indicated below.…”

mentioning

confidence: 99%

Rapid Mercury Assays

Szurdoki

Kido

Hammock

1997

ACS Symposium Series

Self Cite

View full text Add to dashboard Cite

We have developed rapid assays with the potential of being applied to mercury analysis in environmental samples. Our methods combine the simple ELISA-format with the selective, high affinity complexation of mercuric ions by sulfur-containing ligands. The first assay format is based on a sandwich chelate formed by a ligand conjugated to a reporter enzyme, a mercuric ion of the analyzed sample, and a protein bound ligand immobilized on the wells of a microtiter plate. The second assay format involves competition between mercuric ions and an organomercury-conjugate to bind to a chelating conjugate. The assays detect mercuric ions in ppb/high ppt concentrations with high selectivity. Toxicity of MercuryMercury is one of the most hazardous toxic metals. The amount of mercury emitted to the environment is continuously increasing mainly due to industrialization and urbanization. This trend has raised concerns about the risk of human exposure to mercury and the resulting adverse health effects (7).Most of the biological effects of mercury can be explained in terms of the very high affinity of mercurials towards endogenous thiols (7). Toxicologically, the most important mercury-species are elemental (Hg°) and mercuric (Hg 2 " 1 ") mercury, and methylmercury (CH 3 Hg + ). Different forms of mercury exhibit different bioavailabilities, toxic potencies, tissue and organ distributions, and patterns of biological effects (7).Exposure to Hg° vapor is mostly an occupational hazard for dental personnel, workers in the chloralkali industry, plants manufacturing mercury thermometers, and mercury mines (2-4). The toxic impact of the Hg° vapor released from dental amalgam fillings has recently been discussed (2, 4, 5). The initial toxic effects of Hg° are observed in the nervous system and later nephrotoxicity develops (7). Hg°

show abstract

An enzyme immunoassay for the environmental monitoring of the herbicide bromacil

Cited by 35 publications

References 29 publications

Rapid assays for environmental and biological monitoring

Rapid assays for environmental and biological monitoring

Regulatory Application of ELISA: Compliance Monitoring of Bromacil in Soil

Rapid Mercury Assays

Contact Info

Product

Resources

About