An enzyme electrode for the determination of sucrose in food products

Rahni, Mohammad Ali Nabi; Lubrano, G. J.; Guilbault, George G.

doi:10.1021/jf00078a034

Cited by 23 publications

(5 citation statements)

References 6 publications

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“…When the activity of the glucose oxidase was raised from 24 to 151 units, the upper limit of linearity of the calibration curve was extended to 7 X 10~3 M, indicating that the sensitivity of a sucrose electrode increases with the increase of invertase activity. This is in agreement with the results obtained with soluble invertase (15).…”

Section: Resultssupporting

confidence: 93%

“…Although this method is rapid and precise, it is subject to interference originating from the sample matrix. Because immobilized enzyme electrodes offer many potential advantages, a sucrose enzyme electrode was constructed in this laboratory by using an oxygen electrode and three enzymes immobilized on pig intestine membranes (15). The electrode was workable and stable, but its narrow linear range and low sensitivity made it impractical for use in the food industry.…”

mentioning

confidence: 99%

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Sucrose enzyme electrode

Xu¹,

Guilbault²,

Kuan³

1989

Anal. Chem.

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Americans have for a long time used sucrose extensively in most foodstuffs due to their love for sweets. Thus, it is rather important to have a method that is fast, simple, and sensitive and that could be used for the determination of sucrose in foods and agricultural products. The AOAC (Association of Official Analytical Chemists) method is accurate and precise, but very laborious and time-consuming. For some years, there has been a great deal of interest in the development of novel methods for routine analysis of sucrose (1)(2)(3)(4)(5). Several studies on the use of enzyme electrodes for the determination of sucrose have been reported (6,12,(19)(20)(21); however, these sucrose electrodes suffer from lack of either sensitivity or stability. Lately, a flow injection method for sucrose assay has been described (13,14). Although this method is rapid and precise, it is subject to interference originating from the sample matrix. Because immobilized enzyme electrodes offer many potential advantages, a sucrose enzyme electrode was constructed in this laboratory by using an oxygen electrode and three enzymes immobilized on pig intestine membranes (15). The electrode was workable and stable, but its narrow linear range and low sensitivity made it impractical for use in the food industry. Besides, the quality of the pig intestine membrane is difficult to control, thus resulting in poor reproducibility from batch to batch.Instead of a pig intestine membrane, we used more homogeneous artificial membranes, including Teflon, polyethylene, polypropylene, and cellulose acetate, for the construction of glucose and sucrose electrodes. The effects of these membranes and the amount of enzyme(s) immobilized were investigated. The applicability of the sucrose enzyme electrodes to the determination of sucrose in food and food products was evaluated. EXPERIMENTAL SECTIONThe sucrose electrode was constructed by mounting an internal membrane over the tip of an oxygen electrode jacket with an O-ring. Forty microliters of buffer solution containing 0.25 mg of bovine serum albumin (BSA) and various amounts of glucose oxidase, invertase, and mutarotase was placed on the membrane. Then, 10 pL of a 2.5% glutaraldehyde solution was added, with vigorous stirring for 30 s. The immobilized enzyme layer was allowed to form at room temperature for over 3 h. The immobilized enzyme layer was washed several times with phosphate buffer solution. Then an external membrane was placed on the enzyme layer with another O-ring. The excess membrane was cut away with a pair of scissors, and the assembly was tightly sealed with a strip of tape to prevent air from entering the enzyme layer. The completed sucrose electrode was stored in phosphate buffer (pH 6.88, 0.2 M) at 0-4 OC when it was not in use.A glucose electrode was constructed essentially by the same method as described above, except that invertase and mutarotase were not used. Two milliliters of internal filling solution (0.2 M 0003-2700/89/036 1-0782$0 1.50/0 phosphate buffer containing 0.1 M KCl, pH...

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Section: Resultssupporting

confidence: 93%

mentioning

confidence: 99%

Sucrose enzyme electrode

Xu¹,

Guilbault²,

Kuan³

1989

Anal. Chem.

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“…The sensor required conditioning time like typical immobilized enzyme systems [33,59,61]. During this period, which is around three times uses in this study, loosely bound enzymes are washed out and the diffusional channels for substrates transport in the enzyme layer are created [75]. The stability of the sensors based on polymer entrapped laccase or carbon paste mixed laccase was generally about 1-2 weeks [60,61,63,64].…”

Section: Stability Of the Laccase-immobilized Pt Electrodementioning

confidence: 97%

Characterization of an amperometric laccase electrode covalently immobilized on platinum surface

Quan

Kim

Shin

2004

Journal of Electroanalytical Chemistry

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“…Thus, it has been found that there is a maximum loading with bacterial cells (<1.5% of total volume) beyond which the diffusional barrier for oxygen becomes too high (236). Several multienzyme systems have been designed which boost the sensitivity and lower the detection limit through the ping-pong mechanism (222,231,271,272) or where the use of enzymes in a cascade provides some additional selectivity (268,273,274). The whole tissue "bananatrode" [sic] (237) uses banana pulp as the source of tyrosinase for the oxidation of dopamine.…”

Section: Electrochemical Sensorsmentioning

confidence: 99%

“…The whole tissue "bananatrode" [sic] (237) uses banana pulp as the source of tyrosinase for the oxidation of dopamine. There have been number of significant biomedical applications (238,244,245,251,256,259,(275)(276)(277) and applications in food industry (278,273). Enzymatic amperometric electrodes can be used also for determination of enzymatic inhibitors both reversible (279) and irreversible (280).…”

Section: Electrochemical Sensorsmentioning

confidence: 99%