An enzymatic method for glycine

Berger, Sosamma J.; Carter, Joyce A.; Lowry, Oliver H.

doi:10.1016/0003-2697(75)90508-4

Cited by 16 publications

(3 citation statements)

References 5 publications

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“…Glycogen was measured by the method of Passonneau and Lauderdale (1974); glutamine by the method of Young and Lowry (1%); glycine by the method of Berger et al (1975); y-aminobutyrate (GABA) by a modification of the method of Hirsch and Robins (1962); and taurine by the fluorescamine method of Orr e t at. (1976).…”

Section: Measurements Of Metabolitesmentioning

confidence: 99%

Effect of Chronic Hypernatremic Dehydration and Rapid Rehydration on Brain Carbohydrate, Energy, and Amino Acid Metabolism in Weanling Mice

Thurston

Hauhart

Schulz

1983

Journal of Neurochemistry

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This is a study of the effects of chronic hypernatremic dehydration and rehydration on carbohydrate, energy, and amino acid metabolism in the brains of weanling mice. Chronic hypernatremic dehydration induced by 4 days of water deprivation and salt loading was associated with severe weight loss (no other observed clinical effects), increased brain Na+ levels, and a decreased brain water content. Changes in the concentrations of brain glucose, glycolytic and citric acid cycle metabolic intermediates, and phosphocreatine were compatible with reduced cerebral metabolic rate. In adaptation to chronic hypernatremia, there was a significant increase in the content of the measured brain amino acids. Rapid rehydration over a 4-h period with 2.5% dextrose in water returned plasma Na+ levels and brain Na+ and water contents to normal. After rehydration, metabolites were altered in a manner consistent with increased fluxes through the glycolytic pathway and citric acid cycle; the brain glycogen content almost tripled. Brain taurine and glutamine levels were not lowered by rehydration, and the total content of the measured amino acids in brain was still significantly higher than in controls. We speculate that these metabolic perturbations may relate to the development of cerebral edema and seizures or coma following rapid rehydration of humans with chronic hypernatremic dehydration.

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Section: Measurements Of Metabolitesmentioning

confidence: 99%

Effect of Chronic Hypernatremic Dehydration and Rapid Rehydration on Brain Carbohydrate, Energy, and Amino Acid Metabolism in Weanling Mice

Thurston

Hauhart

Schulz

1983

Journal of Neurochemistry

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“…In each assay there are three reaction sequences: a ''specific" reaction that results, either directly or with the aid of an auxiliary enzyme, in the stoichiometric oxidation or reduction of a pyridine nucleotide; an enzymatic cycling amplification step; and an "indicator" step that permits fluorometric measurements of a product of the enzymatic cycling step. The individual methods have been described (glycine: Berger et al, 1975Berger et al, , 1977aGABA: Berger et al, 1977a,b;GAD and GABA-T: Pusateri et al, 1984). However, in the case of single-cell mea-surements, the GABA method described by Berger et al (1977~) for 10-ng samples was modified as follows.…”

Section: Methodsmentioning

confidence: 99%

Distribution of Glycine, γ‐Aminobutyric Acid, Glutamate Decarboxylase, and γ‐Aminobutyric Acid Transaminase in Rabbit and Mudpuppy Retinas

Dick

Lowry

1984

Journal of Neurochemistry

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The distributions of glycine, gamma-aminobutyric acid (GABA), glutamate decarboxylase (EC 4.1.1.15), and GABA transaminase (EC 2.6.1.19) were determined in rabbit and mudpuppy retinas. In both species, peak levels of the amino acids and the enzymes occurred in the inner plexiform layer. Glutamate decarboxylase was almost entirely confined to the inner plexiform layer. Determinations were also made of the GABA content of 107 individual putative amacrine cell somas from mudpuppy retina. About 30% of those somas were found to have high endogenous GABA levels.

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“…Berger et al (18). Heart amino acid concentrations are expressed in millimoles per kilogram of wet weight (ww) and in millimoles per kilogramn of dry weight (dw).…”

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confidence: 99%

Taurine: Possible Role in Osmotic Regulation of Mammalian Heart

Thurston

Hauhart

Naccarato

1981

Science

103

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It is well established that taurine plays an important role in the maintenance of intracellular osmolal concentration in marine invertebrates, teleosts, and amphibians. In fresh water, concentrations of taurine in body tissues decrease; in salt water, they increase. In this study with mice we found that during adaptation of these mammals to chronic hypernatremia, the taurine content of the heart increased; concentrations of other amino acids were unchanged or were decreased. Welty and his associated have shown that acute hyponatremia lowered the taurine concentration of rat heart. In concert, these data suggest that taurine also may serve as an osmotic agent in mammalian heart.

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An enzymatic method for glycine

Cited by 16 publications

References 5 publications

Effect of Chronic Hypernatremic Dehydration and Rapid Rehydration on Brain Carbohydrate, Energy, and Amino Acid Metabolism in Weanling Mice

Effect of Chronic Hypernatremic Dehydration and Rapid Rehydration on Brain Carbohydrate, Energy, and Amino Acid Metabolism in Weanling Mice

Distribution of Glycine, γ‐Aminobutyric Acid, Glutamate Decarboxylase, and γ‐Aminobutyric Acid Transaminase in Rabbit and Mudpuppy Retinas

Taurine: Possible Role in Osmotic Regulation of Mammalian Heart

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