An Environment Friendly, Low-Cost Extraction Process of Phenolic Compounds from Grape Byproducts. Optimization by Multi-Response Surface Methodology

Rajha, Hiba N.; Darra, Nada El; Vorobiev, Eugène; Louka, Nicolas; Maroun, Richard G.

doi:10.4236/fns.2013.46084

Cited by 26 publications

(27 citation statements)

References 40 publications

(54 reference statements)

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“…25 found that antiradical activity of N. sativa extracts increased proportionally to the polyphenol content with a linear relationship between radical inhibition and total polyphenols. Similar studies by 26,27,28 showed concentration-dependent radical scavenging activity of polyphenol extracts.…”

Section: Antiradical Activity Of N Sativa Extracts Against Dpph Radicalsupporting

confidence: 76%

Phytochemical Analysis of Nigella sativa L. Utilizing GC-MS Exploring its Antimicrobial Effects against Multidrug-Resistant Bacteria

Saleh¹,

Darra²,

Raafat³

et al. 2017

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Introduction:The alarming increase in bacterial strains resistant to existing antimicrobial agents has demanded alternative strategies. Medicinal plants are now considered as an alternative treatment because of their secure choice in several diseases. Among them, Nigella sativa is a promising traditional herb having rich medical background. The aim of the study is to perform phytochemical analysis of Nigella sativa L. Utilizing Gas chromatographic-mass spectrometric (GC/MS) exploring its antioxidant and antibacterial activity against multidrug resistant (MDR) gram-positive and gram-negative bacteria. Methods: Total phenolic, tannin, and flavonoid content of N. sativa seed extracts and its commercially available oil were determined. Their radical scavenging activity using DPPH was also tested. The antibacterial activity of N. sativa seed extracts and its oil against MDR gram-positive and gram-negative bacterial strains was studied using disc diffusion test and the biofilm formation assay. GC-MS studies were also performed. Results: Among the different preparations used, N. sativa oil showed the highest antioxidant and antibacterial activity against highly resistant gram-positive bacteria with the greatest suppression of biofilm formation, which was attributed to its high bioactive contents. Conclusion: This study indicates that N. sativa extracts and its oils can be used as natural antibacterial agents to treat infections caused by multidrug resistant bacteria.

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Section: Antiradical Activity Of N Sativa Extracts Against Dpph Radicalsupporting

confidence: 76%

Phytochemical Analysis of Nigella sativa L. Utilizing GC-MS Exploring its Antimicrobial Effects against Multidrug-Resistant Bacteria

Saleh¹,

Darra²,

Raafat³

et al. 2017

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“…The optimal conditions maximizing TPC and AA, in our previous work [15] were 37˚C and 28 hours to obtain 0.52 g GAE/100g DM and 23% respectively. Herein, the optimal extraction conditions obtained for milled pomace were 88˚C and 5 minutes to acquire a TPC and FC of 1.4 g GAE/100g DM, a TMA of 21 mg/100g DM, a TC of 2.2 g/L, an AA of 28% and an AC of 433 mg AAE/L.…”

Section: Multiple Response Optimizationmentioning

confidence: 99%

“…The optimization of aqueous phenolic compounds extraction from milled grape pomace at moderate temperatures and relatively long periods of time [15], was followed by the investigation of the potential capacity of transition to higher temperatures and shorter periods of time in the aim of reducing the overall cost of the process. The optimal conditions maximizing TPC yield, previously found by Rajha et al 2013 in aqueous solution [15] were 47˚C and 30 hours to obtain 0.7 g GAE/100g DM. Herein, we obtained 1.65 g GAE/100g DM at 98˚C after 5 minutes.…”

Section: Effect Of the Extraction Parameters On Tpc And Fcmentioning

confidence: 99%

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Multiple Response Optimization of High Temperature, Low Time Aqueous Extraction Process of Phenolic Compounds from Grape Byproducts

Rajha¹,

Louka²,

Darra³

et al. 2014

FNS

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In this study phenolic compounds extraction from grape byproducts was conducted using pure water as a solvent. High temperatures and low time incubation periods were used in the aim of reducing the cost of the process and heightening the phenolic compounds yield. Response surface methodology (RSM) was realized to study the effect of time and temperature on crushed and uncrushed grape pomace. The phenolic content was evaluated considering the quantity (total phenolics (TPC), flavonoids (FC), total monomeric anthocyanins (TMA) and tannins (TC)), and quality (antiradical activity (AA) and antioxidant capacity (AC)) of the extracts. High temperature low time extraction design used in this study was compared to the extraction process at moderate temperatures with relatively long periods of time. This was proved to ameliorate the quantitative extraction of phenolic compounds from grape pomace without affecting their bioactivity. Moreover, multiple response optimization showed the optimal extraction parameters to be 81˚C and 140 minutes for the unmilled pomace samples, and 88˚C and 5 minutes for the milled. TPC, FC, TMA, TC, AA and AC are almost the same for both optimums. Thus the possibility of replacing the milling process by the extraction time prolongation (for the unmilled pomace) of 135 minutes seems to be very plausible. HPLC analysis showed different quantity and diversity of extracted phenolics for the optimums. However this difference did not significantly affect the overall activity, showing that PC in the different extracts act in complete synergy all together leading to important biological properties. The obtained results using the extraction strategy adopted in this work could lead to several industrial applications.

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“…RSM was demonstrated to be a potent tool in optimizing experimental parameters maximizing numerous responses [11]. Central composite design, which is one of the most common designs, has been widely used to optimize phenolic compounds extraction from grapes [12] and grape pomace [13]. In classical extraction methods, the major parameters believed to affect the effectiveness of the procedure and the quality of the extracts are: temperature, contact time, extraction solvent, solid-liquid ratio, and particle size [14].…”

Section: Introductionmentioning

confidence: 99%

Extraction of Total Phenolic Compounds, Flavonoids, Anthocyanins and Tannins from Grape Byproducts by Response Surface Methodology. Influence of Solid-Liquid Ratio, Particle Size, Time, Temperature and Solvent Mixtures on the Optimization Process

Rajha¹,

Darra²,

Hobaika³

et al. 2014

FNS

Self Cite

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The current work concerns the optimization process of phenolic compounds solid liquid extraction from grape byproducts at high temperatures and short incubation times. The effect of five experimental parameters (solidliquid ratio, particle size, time, temperature and solvent mixture) mostly believed to affect the extraction process was undertaken. A first response surface methodology experimental design was used to optimize the solid-liquid ratio and milling time parameters. A second design was used for the optimization of the quantitative and qualitative parameters. The quantitative parameters studied are: total phenolic compounds, flavonoid content, total monomeric anthocyanin composition and tannin concentration. The qualitative parameters analyzed are: antiradical activity and antioxidant capacity. The second design was based on the use of time, temperature and solvent mixture as optimization parameters. The assays were first conducted separately revealing the best experimental conditions for the maximization of each response variable alone. A simultaneous response surface methodology of all the responses taken together was then conducted, showing the optimal extraction conditions to be: 93 minutes at 94˚C and in 66% ethanol/water solvent. The maximal response values obtained for each parameter are: Total Phenolic Compounds yield (5.5 g GAE/100g DM), Flavonoid Content (5.4 g GAE/100g DM), Total Monomeric Anthocyanin yield (70.3 mg/100g DM), Tannin Concentration (12.3 g/L), Antiradical Activity (67.3%) and Total Antioxidant Capacity (393 mgAAE/L). All of the optimal values were acquired at 3 mL/g solid-liquid ratio and 6.8 min milling time. The obtained extracts could be used as natural bioactive compounds in several industrial applications.

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An Environment Friendly, Low-Cost Extraction Process of Phenolic Compounds from Grape Byproducts. Optimization by Multi-Response Surface Methodology

Cited by 26 publications

References 40 publications

Phytochemical Analysis of Nigella sativa L. Utilizing GC-MS Exploring its Antimicrobial Effects against Multidrug-Resistant Bacteria

Phytochemical Analysis of Nigella sativa L. Utilizing GC-MS Exploring its Antimicrobial Effects against Multidrug-Resistant Bacteria

Multiple Response Optimization of High Temperature, Low Time Aqueous Extraction Process of Phenolic Compounds from Grape Byproducts

Extraction of Total Phenolic Compounds, Flavonoids, Anthocyanins and Tannins from Grape Byproducts by Response Surface Methodology. Influence of Solid-Liquid Ratio, Particle Size, Time, Temperature and Solvent Mixtures on the Optimization Process

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