An ensemble of aptamers and antibodies for multivalent capture of cancer cells

Zhang, Jinling; Sheng, Weian; Fan, Z. Hugh

doi:10.1039/c4cc02002b

Cited by 31 publications

(15 citation statements)

References 29 publications

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“…91 A recently described ensemble of aptamers and antibodies were incorporated into a microfluidic device. 92 This ensemble demonstrated .90% efficiency in capturing CTCs at a flow rate of 2 mL/s of unprocessed blood.…”

Section: Ctc Detection Methodsmentioning

confidence: 94%

Current approaches for avoiding the limitations of circulating tumor cells detection methods—implications for diagnosis and treatment of patients with solid tumors

Kowalik

Kowalewska

Góźdż

2017

Translational Research

136

111

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Eight million people die of cancer each year and 90% of deaths are caused by systemic disease. Circulating tumor cells (CTCs) contribute to the formation of metastases and thus are the subject of extensive research and an abiding interest to biotechnology and pharmaceutical companies. Recent technological advances have resulted in greatly improved CTC detection, enumeration, expansion, and culture methods. However, despite the fact that nearly 150 years have passed since the first detection and description of CTCs in human blood and enormous technological progress that has taken place in this field, especially within the last decade, few CTC detection methods have been approved for routine clinical use. This reflects the substantial methodological problems related to the nature of these cells, their heterogeneity, and diverse metastatic potential. Here, we provide an overview of CTC phenotypes, including the plasticity of CTCs and the relevance of inflammation and cell fusion phenomena for CTC biology. We also review the literature on CTC detection methodology-its recent improvements, clinical significance, and efforts of its clinical application in cancer patients management. At present, CTC detection remains a challenging diagnostic approach as a result of numerous current methodological limitations. This is especially problematic during the early stages of the disease due to the small numbers of CTCs released into the blood of cancer patients. Nonetheless, the rapid development of novel techniques of CTC detection and enumeration in peripheral blood is expected to expedite their implementation in the clinical setting. It is of utmost importance to understand the biology of CTCs and their distinct populations as a prerequisite for achieving this ultimate goal.

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Section: Ctc Detection Methodsmentioning

confidence: 94%

Current approaches for avoiding the limitations of circulating tumor cells detection methods—implications for diagnosis and treatment of patients with solid tumors

Kowalik

Kowalewska

Góźdż

2017

Translational Research

136

111

View full text Add to dashboard Cite

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“…1,2 Different stages of cancer cells have significant mechanical differences due to the stiffness of their cell membrane. 3 Other well-developed methods and techniques based on cell membrane properties, the epithelial cell adhesion molecule (EpCAM) and fluorescence detection for the sorting of cancer cells require specific equipment, such as atomic force microscopy, 3,4 costly antibody reagents, 5,6 and flow cytometry setups. 7–10 Microfluidic chips bring new technologies and methods to both cancer cell analysis and circulating tumor cell (CTC) detection and sorting.…”

mentioning

confidence: 99%

Single-Cell Mechanical Characteristics Analyzed by Multiconstriction Microfluidic Channels

et al. 2017

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A microfluidic device composed of variable numbers of multiconstriction channels is reported in this paper to differentiate a human breast cancer cell line, MDA-MB-231, and a nontumorigenic human breast cell line, MCF-10A. Differences between their mechanical properties were assessed by comparing the effect of single or multiple relaxations on their velocity profiles which is a novel measure of their deformation ability. Videos of the cells were recorded via a microscope using a smartphone, and imported to a tracking software to gain the position information on the cells. Our results indicated that a multiconstriction channel design with five deformation (50 μm in length, 10 μm in width, and 8 μm in height) separated by four relaxation (50 μm in length, 40 μm in width, and 30 μm in height) regions was superior to a single deformation design in differentiating MDA-MB-231 and MCF-10A cells. Velocity profile criteria can achieve a differentiation accuracy around 95% for both MDA-MB-231 and MCF-10A cells.

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“…To improve cell capture efficiency at a higher flow rate, we have also developed a system that combines antibodies with aptamers to form a multivalent affinity surface for tumor cell isolation [96]. As shown in Fig.…”

Section: Methods Of Isolation and Analysismentioning

confidence: 99%

Circulating Tumor Cell Isolation and Analysis

Zhang

Chen

Fan

2016

Advances in Clinical Chemistry

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Isolation and analysis of cancer cells from body fluids have significant implications in diagnosis and therapeutic treatment of cancers. Circulating tumor cells (CTCs) are cancer cells circulating in the peripheral blood or spreading iatrogenically into blood vessels, which is an early step in the cascade of events leading to cancer metastasis. Therefore, CTCs can be used for diagnosing for therapeutic treatment, prognosing a given anticancer intervention, and estimating the risk of metastatic relapse. However, isolation of CTCs is a significant technological challenge due to their rarity and low recovery rate using traditional purification techniques. Recently microfluidic devices represent a promising platform for isolating cancer cells with high efficiency in processing complex cellular fluids, with simplicity, sensitivity, and throughput. This review summarizes recent methods of CTC isolation and analysis, as well as their applications in clinical studies.

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An ensemble of aptamers and antibodies for multivalent capture of cancer cells

Cited by 31 publications

References 29 publications

Current approaches for avoiding the limitations of circulating tumor cells detection methods—implications for diagnosis and treatment of patients with solid tumors

Current approaches for avoiding the limitations of circulating tumor cells detection methods—implications for diagnosis and treatment of patients with solid tumors

Single-Cell Mechanical Characteristics Analyzed by Multiconstriction Microfluidic Channels

Circulating Tumor Cell Isolation and Analysis

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