1992
DOI: 10.1007/bf02276850
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An enhanced liquid chromatographic method for 5-hydroxymethylfurfural determination in UHT milk

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Cited by 50 publications
(26 citation statements)
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“…Traditionally, total HMF determination in milk is carried out by colorimetric measurement at 443 nm of the yellow complex formed with 2thiobarbituric acid (TBA) (Keeney & Bassette, 1959). This colorimetric method suffers a lack of specificity because of the general reactivity of TBA towards aldehydic groups (Burton, 1984), although reversed-phase (RP) HPLC techniques are now available for accurate measurement of HMF in milk (van Boekel & Zia-Ur-Rehman, 1987;Morales et al, 1992).…”
Section: Introductionmentioning
confidence: 99%
“…Traditionally, total HMF determination in milk is carried out by colorimetric measurement at 443 nm of the yellow complex formed with 2thiobarbituric acid (TBA) (Keeney & Bassette, 1959). This colorimetric method suffers a lack of specificity because of the general reactivity of TBA towards aldehydic groups (Burton, 1984), although reversed-phase (RP) HPLC techniques are now available for accurate measurement of HMF in milk (van Boekel & Zia-Ur-Rehman, 1987;Morales et al, 1992).…”
Section: Introductionmentioning
confidence: 99%
“…This colorimetric method lacks specificity because of the general reactivity towards aldehydes (Morales et al, 1996a). However, reverse-phase high-performance liquid chromatography (RP-HPLC) techniques are available for accurate measurement of HMF in milk (Morales et al, 1992).…”
mentioning
confidence: 99%
“…This has been confirmed in model experiments with fruit juices and concentrates as well as in heat-treated milk (Gomis, Alvarez, Naredo, & Alonso, 1991;Kern, 1964;Morales, Romero, & Jimenez-Pe´rez, 1992). The level of HMF in food depends on the equilibrium between destruction by oxidation and formation from precursors (Morales et al, 1997).…”
Section: Hmf Determinationmentioning
confidence: 86%