An enhanced level of LAMP-2A participates in CD4+T cell hyperactivity in patients with primary biliary cholangitis

Abstract

Background: Primary biliary cholangitis (PBC) is an immune-mediated chronic cholestasis, in which T cell homeostasis plays an important role. Lysosomal-associated membrane protein 2 isoform A (LAMP-2A) has been implicated in the regulation of CD4 + T cell responses.Methods: We comprehensively evaluated the immunobiology of CD4 + T cells in patients with PBC (PBC, n=42), chronic hepatitis B (CHB, n=20), and healthy control subjects (HC, n=20) by flow cytometry including activation status and LAMP-2A expression. Additionally, we investigated the activation responses of PBC-naïve CD4 + T cells by stimulation in vitro and tested the changes caused by deleting the gene encoding LAMP-2A.Results: Firstly, we found an increased activation status of circulating CD4 + T cells from PBC patients compared to the HC subjects, and PBC-naïve CD4 + T cells showed enhanced responses after stimulation in vitro. Secondly, PBC-naïve CD4 + T cells expressed a significantly higher level of LAMP-2A compared to the HC and CHB groups [

Activation of natural killer T cells contributes to Th1 bias in the murine liver after 14 d of ethinylestradiol exposure

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