An enhanced immunochromatographic strip test using colloidal gold nanoparticle-labeled dual-type N proteins for detection of antibodies to PRRS virus

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is recognized as one of the most important infectious diseases causing serious economic loss in the swine industry worldwide. Due to its increasing genetic diversity, a rapid and accurate diagnosis is critical for PRRS control. The immunochromatographic strip test (ICST) is a rapid and convenient type of immunoassay. In this study, an on-site immunochromatographic assay-based diagnostic method was developed for detection of PRRS virus (PRRSV)-specific antibodies. The method utilized colloidal gold nanoparticle-labeled dual-type nucleocapsid proteins encoded by open reading frame 7. We evaluated 991 field samples from pig farms and 66 serum samples from experimentally PRRSV-inoculated pigs. Based on true PRRSV-specific antibody-positive or -negative sera determined by immunofluorescence assay and IgM enzyme-linked immunosorbent assay (ELISA), the specificity and sensitivity of the ICST were 97.5% and 91.1%, respectively, similar to those of a commercial ELISA (IDEXX PRRS X3 Ab). More importantly, the ICST was completed within 15 min and could detect the PRRSV-specific antibody at an earlier stage of infection (3–7 days) than that of ELISA (7+ days). The results demonstrate that the developed ICST has great potential as an on-farm diagnostic method, providing excellent diagnostic performance in a quick and convenient manner.

A Novel Immunochromatographic Strip Based on Latex Microspheres for the Rapid Detection of North American-Type Porcine Reproductive and Respiratory Syndrome Virus

“…Virus isolation and RT-PCR are reliable methods for the diagnosis of PRRSV infection, but these methods are mostly completed in laboratories with specialized instruments by skilled technicians. The immunochromatographic strip method based on different labeled tracers and PRRSV proteins has been applied to detect PRRSV antigens or antibodies, and among these methods, colloidal gold technology is the most mature (Zhou et al, 2009;Li et al, 2017;Yu et al, 2018). However, due to the limitations of the characteristics of colloidal gold nanoparticles, their stability and sensitivity are lower than those of some new tracers, such as latex microspheres (Li et al, 2019a).…”

“…The ICS test for detecting PRRSV antibodies or antigens using colloidal gold as the tracer has been reported (Zhou et al, 2009;Li et al, 2017;Yu et al, 2018). However, an immunochromatographic assay based on latex microspheres as the tracer for the detection of PRRSV has not been described.…”

Development and evaluation of a colloidal gold immunochromatographic assay based on recombinant protein CatL1D for serodiagnosis of sheep fasciolosis

“…CGIA is a novel detection method that combines immunological detection methods with chromatography and colloidal gold labelling (Kong et al ., 2017; Yu et al ., 2018). Staphylococcus aureus protein A (SPA) can specifically bind to Fc fragments of IgG in various mammalian serum, and SPA shows strong affinity to IgG.…”

A biosensor for S100B detection based on PSS-MA-GoldMag-LFIA in early clinical diagnosis of brain damage

“…In the past few decades, lateral-flow immunoassay (LFIA) has played a critical role in the application of POCT for its simplicity, speediness, and low cost, which has been utilized in the detection of thrombin, cyromazine and melamine, inflammation biomarkers, virus, and others. [12][13][14][15] Among the various nanomaterials for LFIA, the magnetic nanoparticle is highly preferred for its quantitative and stable signal. 16,17 The magnetic signals throughout the membrane's test line and control line can be entirely detected using a portable magnetic immunochromatography test reader with a higher sensitivity than the conventional optical LFIA system.…”