An engineered pathway for the biosynthesis of renewable propane

Kallio, Pauli T.; Pásztor, András; Thiel, Kati; Akhtar, M. Kalim; Jones, Patrik R.

doi:10.1038/ncomms5731

Cited by 128 publications

(159 citation statements)

References 41 publications

(78 reference statements)

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“…Rodriguez and Atsumi discussed the relevance of their strain for alkane synthesis but did not demonstrate alkane production in their study (56). Production of propane was recently reported by Kallio and colleagues using engineered E. coli that displayed decreased endogenous conversion of butyraldehyde to butanol due to deletions of ahr and yqhD (63).…”

Section: Enhancing Bioconversion Of Aldehydes To Other Chemical Classesmentioning

confidence: 99%

Microbial Engineering for Aldehyde Synthesis

Kunjapur

Prather

2015

Appl Environ Microbiol

145

129

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Aldehydes are a class of chemicals with many industrial uses. Several aldehydes are responsible for flavors and fragrances present in plants, but aldehydes are not known to accumulate in most natural microorganisms. In many cases, microbial production of aldehydes presents an attractive alternative to extraction from plants or chemical synthesis. During the past 2 decades, a variety of aldehyde biosynthetic enzymes have undergone detailed characterization. Although metabolic pathways that result in alcohol synthesis via aldehyde intermediates were long known, only recent investigations in model microbes such as Escherichia coli have succeeded in minimizing the rapid endogenous conversion of aldehydes into their corresponding alcohols. Such efforts have provided a foundation for microbial aldehyde synthesis and broader utilization of aldehydes as intermediates for other synthetically challenging biochemical classes. However, aldehyde toxicity imposes a practical limit on achievable aldehyde titers and remains an issue of academic and commercial interest. In this minireview, we summarize published efforts of microbial engineering for aldehyde synthesis, with an emphasis on de novo synthesis, engineered aldehyde accumulation in E. coli, and the challenge of aldehyde toxicity.

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Section: Enhancing Bioconversion Of Aldehydes To Other Chemical Classesmentioning

confidence: 99%

Microbial Engineering for Aldehyde Synthesis

Kunjapur

Prather

2015

Appl Environ Microbiol

145

129

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“…2D), possibly due to the presence of shorter-chain myristyl-CoA in the peroxisome due to β-oxidation activity. Cytosolic expression of Mycobacterium marinum carboxylic acid reductase MmCAR (24) along with an ACP activation module, BsuSfp [phosphopantetheinyl transferase from Bacillus subtilis (19)], and PmADO resulted in an alkane production of 23.3 mg/L (Fig. 2D), suggesting that the endogenous fatty acid pool may be an alternative route to synthesizing alkane fuels.…”

Section: Mg/lmentioning

confidence: 99%

Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals

Qiao

Ahn

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

382

305

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Harnessing lipogenic pathways and rewiring acyl-CoA and acyl-ACP (acyl carrier protein) metabolism in Yarrowia lipolytica hold great potential for cost-efficient production of diesel, gasoline-like fuels, and oleochemicals. Here we assessed various pathway engineering strategies in Y. lipolytica toward developing a yeast biorefinery platform for sustainable production of fuel-like molecules and oleochemicals. Specifically, acyl-CoA/acyl-ACP processing enzymes were targeted to the cytoplasm, peroxisome, or endoplasmic reticulum to generate fatty acid ethyl esters and fatty alkanes with tailored chain length. Activation of endogenous free fatty acids and the subsequent reduction of fatty acyl-CoAs enabled the efficient synthesis of fatty alcohols. Engineering a hybrid fatty acid synthase shifted the free fatty acids to a medium chain-length scale. Manipulation of alternative cytosolic acetyl-CoA pathways partially decoupled lipogenesis from nitrogen starvation and unleashed the lipogenic potential of Y. lipolytica. Taken together, the strategies reported here represent promising steps to develop a yeast biorefinery platform that potentially upgrades low-value carbons to high-value fuels and oleochemicals in a sustainable and environmentally friendly manner.

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“…In order to produce propane by the recombinant E. coli, a synthetic pathway for propane biosynthesis was constructed by expression of the thioesterase gene from Bacteroides fragilis, the carboxylic acid reductase gene from Mycobacterium marinum, the mature phosphopantetheinyl transferase gene from Bacillus subtilis, and the aldehyde deformylating oxygenase gene from Prochlorococcus marinus. After optimization of the culturing conditions, the recombinant E. coli produced 32.0 mg/l of propane within 19 h with a productivity of 0.0017 g/l/h and a yield of 0.0016 g/g of glucose (Kallio et al 2014).…”

Section: Genetically Modified E Colimentioning

confidence: 99%

Hydrocarbons, the advanced biofuels produced by different organisms, the evidence that alkanes in petroleum can be renewable

Chi

et al. 2015

Appl Microbiol Biotechnol

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It is generally regarded that the petroleum cannot be renewable. However, in recent years, it has been found that many marine cyanobacteria, some eubacteria, engineered Escherichia coli, some endophytic fungi, engineered yeasts, some marine yeasts, plants, and insects can synthesize hydrocarbons with different carbon lengths. If the organisms, especially some native microorganisms and engineered bacteria and yeasts, can synthesize and secret a large amount of hydrocarbons within a short period, alkanes in the petroleum can be renewable. It has been documented that there are eight pathways for hydrocarbon biosynthesis in different organisms. Unfortunately, most of native microorganisms, engineered E. coli and engineered yeasts, only synthesize a small amount of intracellular and extracellular hydrocarbons. Recently, Aureobasidium pullulans var. melanogenum isolated from a mangrove ecosystem has been found to be able to synthesize and secret over 21.5 g/l long-chain hydrocarbons with a yield of 0.275 g/g glucose and a productivity of 0.193 g/l/h within 5 days. The yeast may have highly potential applications in alkane production.

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An engineered pathway for the biosynthesis of renewable propane

Cited by 128 publications

References 41 publications

Microbial Engineering for Aldehyde Synthesis

Microbial Engineering for Aldehyde Synthesis

Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals

Hydrocarbons, the advanced biofuels produced by different organisms, the evidence that alkanes in petroleum can be renewable

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