An endothelial receptor for oxidized low-density lipoprotein

Abstract

Endothelial dysfunction or activation elicited by oxidatively modified low-density lipoprotein (Ox-LDL) has been implicated in the pathogenesis of atherosclerosis, characterized by intimal thickening and lipid deposition in the arteries. Ox-LDL and its lipid constituents impair endothelial production of nitric oxide, and induce the endothelial expression of leukocyte adhesion molecules and smooth-muscle growth factors, which may be involved in atherogenesis. Vascular endothelial cells in culture and in vivo internalize and degrade Ox-LDL through a putative receptor-mediated pathway that does not involve macrophage scavenger receptors. Here we report the molecular cloning, using expression cloning strategy, of an Ox-LDL receptor from vascular endothelial cells. The cloned receptor is a membrane protein that belongs structurally to the C-type lectin family, and is expressed in vivo in vascular endothelium and vascular-rich organs.

The centromeric part of the human NK gene complex: linkage of LOX-1 and LY49L with the CD94/NKG2 region

“…Most parts of the region are covered by several clones (see Figure 1). The LOX-1 gene 29 was detected in clone L21228, the first clone on the telomeric side of this contig, and this gene is therefore located about 100 kb telomeric of the STS marker D12S77, which flanks the NKG2/CD94 region. The CD94 gene and all five NKG2 genes are placed between 100 to 200 kb centromeric of D12S77 and their relative orientation and linkage has been described previously.…”

“…28 Furthermore, a member of another lectin-type receptor class, LOX-1, has been implicated as a receptor for oxidized low-density lipoprotein on endothelial cells and monocytes. 29 In order to establish the basis to search for novel forms of lectin-type receptor genes, to link the NKG2 and CD94 NK cell receptor genes 13,23 with the region containing the LY49L and other potentially related genes and to get some clues to the evolution of the locus, we have now constructed a physical map based on PAC and BAC clones of the centromeric part of the NK complex region. We have focused here on the centromeric part of the NK gene complex since the available data indicate that the syntenic region of rodents contains more than nine Ly49 genes and resistance loci for cytomegalovirus and pox virus.…”

Design of a novel LOX-1 receptor antagonist mimicking the natural substrate

“…The lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), located on the surface of vascular endothelial cells, is the major receptor for oxidized low-density lipoprotein (ox-LDL) [1,2]. It is a membrane glycoprotein, up-regulated by ox-LDL, expressed also in monocytes/macrophages, smooth muscle cells, dendritic cells and fibroblasts.…”

Induction of bovine articular chondrocyte senescence with oxidized low‐density lipoprotein through lectin‐like oxidized low‐density lipoprotein receptor 1

“…Anti-Akt rabbit IgG, antiphosphorylated-Akt (anti-pAkt; Ser 473 ) rabbit IgG, and antirabbit IgG horseradish peroxidase-linked antibodies were purchased from Cell Signaling Technology (Beverly, MA). The methods we used to prepare the native LDL and ox-LDL have been described previously (2,11).…”

“…A novel receptor for ox-LDL, designated lectin-like ox-LDL receptor 1 (LOX-1), was recently cloned from cultured bovine aortic endothelial cells (2). The uptake of ox-LDL through this receptor, which is expressed on the vascular endothelium, is critically involved in endothelial activation and dysfunction in atherogenesis (3).…”