An endornavirus from a hypovirulent strain of the violet root rot fungus, Helicobasidium mompa

Osaki, Hideki; Nakamura, Hitoshi; Sasaki, Atsuko; Matsumoto, Naoyuki; Yoshida, Kōji

doi:10.1016/j.virusres.2005.12.004

Cited by 57 publications

(50 citation statements)

References 22 publications

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“…Almost all of the 14 000-18 000 nucleotides comprising endornavirus genomes code for a putative polyprotein and exhibit a characteristic nick near the 59 end of the coding strand. Similar putative viruses have been found in the Stramenopila (Phytophthora; Hacker et al, 2005) and in the basidiomycete Helicobasidium mompa (Fukuhara et al, 2005;Osaki et al, 2006), both of which are pathogens of plants.…”

Section: Introductionsupporting

confidence: 62%

“…Other highly similar regions were found among assigned members of the genus Endornavirus [Vicia faba endornavirus (VFV; Pfeiffer et al, 1993), Oryza sativa endornavirus (OSV; Fukuhara et al, 1995) and Oryza rufipogon endornavirus (ORV; Moriyama et al, 1999)], two unclassified members of Phytophthora endornavirus 1 (PEV1; Hacker et al, 2005) as well as Helicobasidium mompa endornavirus (HmEV1-670; Osaki et al, 2006). The expect value of these viruses ranged from 2E-72 to 3E-19.…”

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confidence: 83%

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A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities

Tuomivirta¹,

Kaitera²,

Hantula³

2009

Journal of General Virology

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A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities Ascospore and mycelial isolates of Gremmeniella abietina type B were found to contain three different dsRNA molecules with approximate lengths of 11, 5 and 3 kb. The 11 kb dsRNA encoded the genome of a putative virus and is named Gremmeniella abietina type B RNA virus XL (GaBRV-XL). GaBRV-XL probably exists in an unencapsulated state. We identified two distinct dsRNAs (10 374 and 10 375 bp) of GaBRV-XL, both of which coded for the same putative polyprotein (3249 amino acids) and contained four regions similar to putative viral methyltransferases, DExH box helicases, viral RNA helicase 1 and RNA-dependent RNA polymerases. While a cysteine-rich region with several CxCC motifs in GaBRV-XL was similar to that of putative endornaviruses, cluster analyses of conserved regions revealed GaBRV-XL to be distinct from a broad range of viral taxa but most closely related to Discula destructiva virus 3. Collectively, these findings suggest that GaBRV-XL represents a novel virus group related to endornaviruses. INTRODUCTIONRecently recognized by the International Committee on Taxonomy of Viruses (ICTVdB Management, 2006), endornaviruses are usually cryptic and non-enveloped plant and fungal dsRNA viruses that spread efficiently through mitotic and meiotic cells but not through grafts (Fukuhara et al., 1995;Moriyama et al., 1999;Wakarchuk & Hamilton, 1990;Pfeiffer, 1998 Fungal viruses of the G. abietina species complex have been studied thoroughly in type A and are known to include putative members of the virus families Narnaviridae, Totiviridae and Partitiviridae, some of which can co-infect a single fungal isolate (Tuomivirta et al., 2002;Tuomivirta & Hantula, 2005).The goals of this study include a survey of dsRNA molecules in G. abietina type B, molecular characterization of the most common dsRNA type and its comparison to representatives of similar viral taxa. METHODSG. abietina type B strains. G. abietina type B strains (see Supplementary Table S1, available in JGV Online) were identified by the random amplified microsatellite (RAMS) technique of Hantula & Müller (1997). Branches from P. sylvestris and Pinus contorta ,2 m in height (,20 years old) with symptoms of scleroderris canker were collected in artificially or naturally regenerated stands between June 1994 and June 1995 in northern Finland. Disease symptoms included death of lateral branches over several internodes, cankers, yellowgreen woody tissues, pycnidia and apothecia. Fungi were isolated either from pycnidia or from infected wood adjacent to apothecia.Nucleic acid isolation and electrophoresis. G. abietina type B isolates were grown at 20 uC on modified orange serum agar covered with a cellophane membrane (Müller et al., 1994). dsRNA isolationThe GenBank/EMBL/DDBJ accession numbers for the complete sequences of the 11 kb dsRNAs of isolates AU58 (GaBRV-XL1) and E46 (GaBRV-XL2) are respectively DQ399289 and DQ399290.Details of ...

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Section: Introductionsupporting

confidence: 62%

mentioning

confidence: 83%

A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities

Tuomivirta¹,

Kaitera²,

Hantula³

2009

Journal of General Virology

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“…Some partitiviruses infect fruit-pathogenic fungi, e.g., Helicobasidium mompa virus (HmV) (6,17,51), Rosellinia necatrix partitivirus 1 (RnPV1) (17), RnPV2 (32), and tentative species RnPV3, RnPV4, and RnPV5 (52), while no chrysovirus is known to infect fruit-pathogenic fungi (12)(13)(14)(15)(16)(17)45). To our knowledge, this is the first report of a chrysovirus and a partitivirus infecting B. dothidea and the first report of a chrysovirus associated with the hypovirulence of a phytopathogenic fungus (50).…”

Section: Discussionmentioning

confidence: 99%

“…Biocontrol measures may represent an important way to combat fungal diseases. Apart from R. necatrix and Helicobasidium mompa, no other fungi attacking fruit trees have been reported to be infected by mycoviruses (6,(11)(12)(13)(14)(15)(16)(17). Here we report on a strain of B. dothidea (LW-1) isolated from a pear tree from Wuhan, China.…”

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confidence: 95%

Hypovirulence of the Phytopathogenic Fungus Botryosphaeria dothidea: Association with a Coinfecting Chrysovirus and a Partitivirus

Wang

Jiang

Wang

et al. 2014

J Virol

102

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Botryosphaeria dothidea is an important pathogenic fungus causing fruit rot, leaf and stem ring spots and dieback, stem canker, stem death or stool mortality, and decline of pear trees. Seven double-stranded RNAs (dsRNAs; dsRNAs 1 to 7 with sizes of 3,654, 2,773, 2,597, 2,574, 1,823, 1,623, and 511 bp, respectively) were identified in an isolate of B. dothidea exhibiting attenuated growth and virulence and a sectoring phenotype. Characterization of the dsRNAs revealed that they belong to two dsRNA mycoviruses. The four largest dsRNAs (dsRNAs 1 to 4) are the genomic components of a novel member of the family Chrysoviridae (tentatively designated Botryosphaeria dothidea chrysovirus 1 [BdCV1]), a view supported by the morphology of the virions and phylogenetic analysis of the putative RNA-dependent RNA polymerases (RdRps). Two other dsRNAs (dsRNAs 5 and 6) are the genomic components of a novel member of the family Partitiviridae (tentatively designated Botryosphaeria dothidea partitivirus 1 [BdPV1]), which is placed in a clade distinct from other established partitivirus genera on the basis of the phylogenetic analysis of its RdRp. The smallest dsRNA, dsRNA7, seems to be a noncoding satellite RNA of BdPV1 on the basis of the conservation of its terminal sequences in BdPV1 genomic segments and its cosegregation with BdPV1 after horizontal transmission. This is the first report of a chrysovirus and a partitivirus infecting B. dothidea and of a chrysovirus associated with the hypovirulence of a phytopathogenic fungus. IMPORTANCEOur studies identified and characterized two novel mycoviruses, Botryosphaeria dothidea chrysovirus 1 (BdCV1) and Botryosphaeria dothidea partitivirus 1 (BdPV1), associated with the hypovirulence of an important fungus pathogenic to fruit trees. This is the first report of a chrysovirus and a partitivirus infecting B. dothidea and of a chrysovirus associated with the hypovirulence of a phytopathogenic fungus. BdCV1 appears to be a good candidate for the biological control of the serious disease induced by B. dothidea. Additionally, BdPV1 is placed in a clade distinct from the established genera. The BdCV1 capsid has two major structural proteins, and the capsid is distinct from that made up by a single polypeptide of the typical chrysoviruses. BdPV1 is the second partitivirus in which the putative capsid protein shares no significant identity with any mycovirus protein.A small accompanying dsRNA that is presumed to be a noncoding satellite RNA of BdPV1 is the first of its kind reported for a partitivirus.

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“…Therefore, transcription factors regulated by protein G are downregulated, including CpST12, which is necessary for fungal virulence and expression of genes that respond to hypovirulence (Deng et al, 2007). Hypovirulent strains of phytopathogenic fungi have been reported in several species such as Fusarium oxysporum (Kilic & Griffin, 1998), Rhizoctonia solani (Liu et al, 2003a;Cardinale et al, 2006) Sclerotinia sclerotiorum (Li et al, 2003), Helicobasidium mompa (Osaki et al, 2006), Botrytis cinerea (Wu et al, 2007), and Fusarium graminearum (Kwon et al, 2009). The best examples have been described in Cryphonectria parasitica, the causal agent of chestnut blight, a devastating disease of the American chestnut tree (Castanea dentata [Marsh] Borkh) (Anagnostakis & Day, 1979;Rae et al, 1989;Liu et al, 2003b;Lin et al, 2007;Turchetti et al, 2008).…”

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confidence: 99%

Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree

Figueirêdo

Giancoli

et al. 2012

Trop. plant pathol.

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Fungi are disease-causing agents in plants and affect crops of economic importance. One control method is to induce resistance in the host by using biological control with hypovirulent phytopathogenic fungi. Here, we report the detection of a mycovirus in a strain of Colletotrichum gloeosporioides causing anthracnose of cashew tree. The strain C. gloeosporioides URM 4903 was isolated from a cashew tree (Anacardium occidentale) in Igarassu, PE, Brazil. After nucleic acid extraction and electrophoresis, the band corresponding to a possible double-stranded RNA (dsRNA) was purified by cellulose column chromatography. Nine extrachromosomal bands were obtained. Enzymatic digestion with DNAse I and Nuclease S1 had no effect on these bands, indicating their dsRNA nature. Transmission electron microscopic examination of extracts from this strain showed the presence of isometric particles (30-35 nm in diameter). These data strongly suggest the infection of this C. gloeosporioides strain by a dsRNA mycovirus. Once the hypovirulence of this strain is confirmed, the strain may be used for the biological control of cashew anthracnose. Key words: Anacardium occidentale, anthracnose, biological control, hypovirulence, mycovirus, phytopathogenic fungi.Fungi are the most important disease-causing agents in plants with serious economic impact. Chemical methods to control phytopathogenic fungi can affect human health and have environmental consequences. In an attempt to minimize dependence on chemical methods, alternative means of pathogen control have been investigated (Yu et al., 2010). One alternative is to use hypovirulent phytopathogenic fungal strains that can biologically control phytopathogenic fungi of the same or related species. Fungal hypovirulence is usually caused by mycoviral infection. Hypovirulent fungi cause milder infection and may induce hypovirulence in other pathogenic strains via viral transfer (Pearson et al., 2009).All currently known mycoviruses have isometric, double-stranded RNA (dsRNA)-containing particles, and are classified into three families: Chrysoviridae, Endornaviridae, Partitiviridae and Totiviridae. Particle size ranges from 25 to 50 nm in diameter, except for endornaviruses which do not produce virions (Chu et al., 2002;Ghabrial & Suzuki, 2009).In this communication, we report the presence of isometric dsRNA viral particles in a strain of Colletotrichum gloeosporioides (Penz.) Sacc., the causal agent of anthracnose in cashew tree.Colletotrichum gloeosporioides URM 4903 was isolated from anthracnose lesions on a cashew tree (Anacardium occidentale L.) in the town of Igarassu, Pernambuco, Brazil (7°50'3.74"S and 34°54'22.87"O), and deposited in the mycology collection (URM) at Federal University of Pernambuco, Recife, PE. This strain was chosen after preliminary tests revealed poor pathogenicity in detached cashew leaves and the presence of extrachromosomal bands in agarose gel electrophoresis (unpublished data).PDA (20% potato extract, 2% dextrose, 1.5% agar) and PD (20% potato extract,...

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An endornavirus from a hypovirulent strain of the violet root rot fungus, Helicobasidium mompa

Cited by 57 publications

References 22 publications

A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities

A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities

Hypovirulence of the Phytopathogenic Fungus Botryosphaeria dothidea: Association with a Coinfecting Chrysovirus and a Partitivirus

Detection of isometric, dsRNA-containing viral particles in Colletotrichum gloeosporioides isolated from cashew tree

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