An endophytic Neurospora sp. from Nothapodytes foetida producing camptothecin

Rehman, Suriya; Shawl, A. S.; Kour, Amardeep; Andrabi, Riyaz; Sudan, P.; Sultan, Phalisteen; Verma, Vivek; Qazi, Ghulam N.

doi:10.1134/s0003683808020130

Cited by 118 publications

(59 citation statements)

References 12 publications

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“…It is generally recognized that endophytes represent an important and largely untapped reservoir of unique chemical structures that have been modified through evolution (Gunatilaka 2006;Kharwar et al 2011) and exhibit the capability to produce the same functional compounds as their hosts, some examples include taxol (Stierle et al 1993(Stierle et al , 1995, podophyllotoxin , hypericin (Kusari et al 2008), and azadirachtin (Findlay et al 1997). In 2005, Puri et al reported the first discovery of CPT-producing endophytic fungus Entrophospora infrequens from the inner bark of N. foetida, which set the stage for a more comprehensive examination of other plants for the presence of CPT-producing endophytes Rehman et al 2008Rehman et al , 2009Kusari et al 2009;Shweta et al 2010Shweta et al , 2013Gurudatt et al 2010). These intriguing results have undoubtedly renewed the interests in exploring other endophytic sources of CPT and its analogues from various plant sources.…”

Section: Introductionmentioning

confidence: 99%

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Xiang

Xing

Chen

et al. 2013

Appl Microbiol Biotechnol

148

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Camptothecin (CPT), the third largest anticancer drug, is produced mainly by Camptotheca acuminata and Nothapodytes foetida. CPT itself is the starting material for clinical CPT-type drugs, but the plant-derived CPT cannot support the heavy demand from the global market. Research efforts have been made to identify novel sources for CPT. In this study, three CPT-producing endophytic fungi, Aspergillus sp. LY341, Aspergillus sp. LY355, and Trichoderma atroviride LY357, were isolated and identified from C. acuminata. Most CPT produced by these fungi was found in the fermentation broth, and their corresponding CPT yields were 7. 93, 42.92, and 197.82 μg l −1 , respectively. The CPT-producing capability of LY341 and LY355 was completely lost after repeat subculturing. A substantial decrease of CPT production was also observed in the second generation of LY357. However, a stable and sustainable production of CPT was found from the second generation through the eighth generation of LY357. The fermentation medium, time, pH, temperature, and agitation rate were optimized for CPT production. Methyl jasmonate and XAD16 were proven to be an optimum elicitor and adsorbent resin, respectively, in view of that CPT yield was increased 3.4-and 11-fold through their use. A 50-to 75-fold increase of CPT yield was obtained when the optimized fermentation conditions, elicitor, and adsorbent resin were combined and applied to the culture of the seventh and eighth generations of LY357, and the highest CPT yield was 142.15 μg l −1 . The CPTproducing T. atroviride LY357 paves a potential to uncover the mysteries of CPT biosynthesis.

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Section: Introductionmentioning

confidence: 99%

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Xiang

Xing

Chen

et al. 2013

Appl Microbiol Biotechnol

148

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“…N. foetida [290] CPT Nodulisporium sp. N. foetida [291] CPT, HCPT, MCPT Fusarium solani Camptotheca acuminata [292] CPT, HCPT, MCPT F. solani Apodytes dimidiata [293] CPT Botryosphaeria parva Nothapodytes nimmoniana [294] CPT Diaporthe conorum N. nimmoniana [294] CPT Fusarium oxysporum N. nimmoniana [294] CPT Fusarium sacchari N. nimmoniana [294] CPT F. solani N. nimmoniana [294] CPT Fusarium sp.…”

Section: Entrophospora Infrequensmentioning

confidence: 99%

Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of Spermatophyta

2015

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Abstract:It is known that plant-based ethnomedicine represented the foundation of modern pharmacology and that many pharmaceuticals are derived from compounds occurring in plant extracts. This track still stimulates a worldwide investigational activity aimed at identifying novel bioactive products of plant origin. However, the discovery that endophytic fungi are able to produce many plant-derived drugs has disclosed new horizons for their availability and production on a large scale by the pharmaceutical industry. In fact, following the path traced by the blockbuster drug taxol, an increasing number of valuable compounds originally characterized as secondary metabolites of plant species belonging to the Spermatophyta have been reported as fermentation products of endophytic fungal strains. Aspects concerning sources and bioactive properties of these compounds are reviewed in this paper.

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“…Several isolates belonged to the Hypoxylon, Daldinia, Xylaria and Nodulisporium genera [28] . In Nothapodytes foetida, Nodulisporium species were identified using 28S DNA sequencing [29] . According to Huang, [30] Xylaria species are the dominant fungal endophytes in the genus Artemisia (Asteraceae).…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of endophytic fungi in the medicinal plant Mikania laevigata (Asteraceae)

Ribeiro¹,

Kamida²,

Almeida³

et al. 2014

Phcog J.

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The Mikania laevigata (Asteraceae) is a Brazilian medicinal plant used to treat respiratory tract diseases. It is produced on a large scale for phytotherapic formulation. No studies on the endophytes of Mikania laevigata have been described in the literature. The endophytic fungi isolated from the leaves of M. laevigata cultivated in a floral garden located in the south region of the Bahia state, Brazil, were investigated. After surface disinfection, the performing the isolation procedure, a total of forty endophytic fungi were obtained and grouped into different morphospecies. The fungi were characterized by molecular sequencing the ITS rDNA regions, and they were identified by comparing the results published Gen Bank sequences.The phylogenetic analyses revealed four major clades of fungi from the sequenced ITS-rDNA regions: Nodulisporium sp. (3 isolates), Hypoxylon sp. (3 isolates), Daldinia sp. (1 isolate) and Xylaria luteostromata (aunique isolate).The Xylariaceae Genus was the dominant group of fungi associated with Mikania laevigata.

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An endophytic Neurospora sp. from Nothapodytes foetida producing camptothecin

Cited by 118 publications

References 12 publications

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of Spermatophyta

Isolation and identification of endophytic fungi in the medicinal plant Mikania laevigata (Asteraceae)

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