An endophytic diatom,<i>Pseudogomphonema</i>sp. (Naviculaceae, Bacillariophyceae), lives inside the red alga<i>Neoabbottiella</i>(Halymeniaceae, Rhodophyta)

Klochkova, Tatyana A.; Pisareva, N. A.; Park, Joon‐Sang; Lee, Jin Hwan; Han, Jong Won; Клочкова, Н. Г.; Kim, Gwang Hoon

doi:10.2216/13-229.1

Cited by 11 publications

(5 citation statements)

References 34 publications

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“…Several of the OTUs from our study, which were taxonomically and phylogenetically assigned to different species of the orders Naviculales, Cocconeidales, and Fragilariales (Figure 6), were present in multiple samples/hosts independent of geographic location. Our molecular data in combination with previous observational studies (Baardseth, 1969;Baardseth & Taasen, 1973;Hasle, 1968;Klochkova et al, 2014;Mayombo et al, 2019Mayombo et al, , 2020Totti et al, 2009) support the hypothesis that some diatom species such as Navicula sp. and Cocconeis sp.…”

Section: Microeukaryotes In the Seaweed Holobiontsupporting

confidence: 88%

“…Diatoms are regularly observed in the biofilm on seaweed surfaces, where they live as epiphytes and often occur in large numbers (Costa et al, 2016; Lage & Graça, 2016; Tiffany, 2011; Totti et al, 2009). Although many diatoms associated with brown algae are epiphytes, there are also studies that have demonstrated that certain diatom species, such as the ones belonging to the genera Navicula and Cocconeis can live as endophytes, intercellularly in tissues of both brown algae and red algae (Baardseth, 1969; Baardseth & Taasen, 1973; Hasle, 1968; Klochkova et al, 2014). Recent studies have suggested that some diatom taxa might have adapted to an endophytic life style to avoid the antifouling mechanisms of their hosts (Mayombo et al, 2019, 2020).…”

Section: Discussionmentioning

confidence: 99%

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18S rDNA gene metabarcoding of microeukaryotes and epi‐endophytes in the holobiome of seven species of large brown algae

Bjorbækmo,

Brodie,

Krabberød

et al. 2023

Journal of Phycology

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Brown algae (Phaeophyceae) are habitat‐forming species in coastal ecosystems and include kelp forests and seaweed beds that support a wide diversity of marine life. Host‐associated microbial communities are an integral part of phaeophyte biology, and whereas the bacterial microbial partners have received considerable attention, the microbial eukaryotes associated with brown algae have hardly been studied. Here, we used broadly targeted “pan‐eukaryotic” primers (metabarcoding) to investigate brown algal‐associated eukaryotes (the eukaryome). Using this approach, we aimed to investigate the eukaryome of seven large brown algae that are important and common species in coastal ecosystems. We also aimed to assess whether these macroalgae harbor novel eukaryotic diversity and to ascribe putative functional roles to the host‐associated eukaryome based on taxonomic affiliation and phylogenetic placement. We detected a significant diversity of microeukaryotic and algal lineages associated with the brown algal species investigated. The operational taxonomic units (OTUs) were taxonomically assigned to 10 of the eukaryotic major supergroups, including taxonomic groups known to be associated with seaweeds as epibionts, endobionts, parasites, and commensals. Additionally, we revealed previously unrecorded sequence types, including novel phaeophyte OTUs, particularly in the Fucus spp. samples, that may represent fucoid genomic variants, sequencing artifacts, or undescribed epi‐/endophytes. Our results provide baseline data and technical insights that will be useful for more comprehensive seaweed eukaryome studies investigating the evidently lineage‐rich and functionally diverse symbionts of brown algae.

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Section: Microeukaryotes In the Seaweed Holobiontsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

18S rDNA gene metabarcoding of microeukaryotes and epi‐endophytes in the holobiome of seven species of large brown algae

Bjorbækmo,

Brodie,

Krabberød

et al. 2023

Journal of Phycology

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“…It is possible that the clavate elongated shape of the diatom frustules represents functional morphology that facilitates spacing of the host cells and thus colonisation of the deeper tissue layers. As previously observed by Klotchkova et al [62], the tissue-boring diatoms, despite causing visible tissue damage and often inducing the so-called warping of the thallus, do not seem to change the host physiology or affect its growth rates, and their ecological role is not well understood. It has been suggested that growth of apochlorotic and mixotrophic macroalgae-associated endophytic diatoms is enhanced by significant amounts of extracellular carbon released from the damaged and decaying thallus [63][64][65].…”

Section: Discussionmentioning

confidence: 91%

“…Klotchkova et al [62] showed that tissue damage in two species of the red algae Neoabbottiella Perestenko was linked to the presence of a diatom identified as Pseudogomphonema sp., that developed both in the medullar layer and cortex of the host macroalgae as well as on its surface. Both endo-and epi-phytic forms were normally pigmented and most likely photosynthetically active, despite the very low light intensities transmitted into the deeper layers of the thallus, leading the authors to propose a mixotrophic mode of nutrition for this diatom taxon [62]. We speculate that tissue-boring taxa observed in the current study that share the "gomphonemoid" shape and growth form with the endobiotic Pseudogomphonema sp.…”

Section: Discussionmentioning

confidence: 99%

An Assessment of the Influence of Host Species, Age, and Thallus Part on Kelp-Associated Diatoms

2020

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Diatom community composition and abundances on different thallus parts of adult and juvenile specimens of Eckloniamaxima and Laminariapallida were examined in False Bay, South Africa, using light and scanning electron microscopy. Altogether, 288 thallus portions were analysed. Diatom abundances ranged from 0 to 404 cells mm−2 and were generally higher on E. maxima and juvenile thalli than L. pallida and adult specimens. Moreover, diatom abundances differed between the various thallus parts, being highest on the upper blade and lowest on the primary blade. A total of 48 diatom taxa belonging to 28 genera were found. Gomphoseptatum Medlin, Nagumoea Witkowski and Kociolek, Cocconeis Ehrenberg, and Navicula Bory were the most frequently occurring genera, being present in 84%, 65%, 62.5%, and 45% of the analysed samples, respectively. Among these, Cocconeis and Gomphoseptatum were the most abundant, contributing 50% and 27% of total diatom cells counted collectively across all samples. Permutational multivariate analysis of variance (PERMANOVA) revealed that all investigated main factors (kelp species and age and thallus part), as well as their two- and three-way interactions, except for the interaction between the host species and age, were significant. The high residual variance (72%) indicated that the sum of other unexamined factors contributed the largest component of the variation observed in the kelp-associated diatom communities, and grazing and possible defence strategies utilised by kelps are proposed as processes playing an important role in the structuring of epiphytic diatom communities. Possible endophytism of tissue-boring diatoms colonizing both kelp species is briefly discussed.

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“…Pseudogomphonema sp. 1 was smaller than Pseudogomphonema plinskii Witkowski, Metzelin & Lange-Bertalot and the endophytic diatom found inside the macroalgal genus Neoabbottiella [ 49 ] and could be yet undescribed . In contrast with the epiphytic diatom studies, usual proxies for sea-ice as Fragilariopsis curta [ 50 ] and Thalassiosira antarctica [ 51 ] were not found in as much predominance as, for instance, in Majewska et al [ 3 ].…”

Section: Discussionmentioning

confidence: 99%

Epiphytic diatom community structure and richness is determined by macroalgal host and location in the South Shetland Islands (Antarctica)

et al. 2021

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The marine waters around the South Shetland Islands are paramount in the primary production of this Antarctic ecosystem. With the increasing effects of climate change and the annual retreat of the ice shelf, the importance of macroalgae and their diatom epiphytes in primary production also increases. The relationships and interactions between these organisms have scarcely been studied in Antarctica, and even less in the volcanic ecosystem of Deception Island, which can be seen as a natural proxy of climate change in Antarctica because of its vulcanism, and the open marine system of Livingston Island. In this study we investigated the composition of the diatom communities in the context of their macroalgal hosts and different environmental factors. We used a non-acidic method for diatom digestion, followed by slidescanning and diatom identification by manual annotation through a web-browser-based image annotation platform. Epiphytic diatom species richness was higher on Deception Island as a whole, whereas individual macroalgal specimens harboured richer diatom assemblages on Livingston Island. We hypothesize this a possible result of a higher diversity of ecological niches in the unique volcanic environment of Deception Island. Overall, our study revealed higher species richness and diversity than previous studies of macroalgae-inhabiting diatoms in Antarctica, which could however be the result of the different preparation methodologies used in the different studies, rather than an indication of a higher species richness on Deception Island and Livingston Island than other Antarctic localities.

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An endophytic diatom,Pseudogomphonemasp. (Naviculaceae, Bacillariophyceae), lives inside the red algaNeoabbottiella(Halymeniaceae, Rhodophyta)

Cited by 11 publications

References 34 publications

18S rDNA gene metabarcoding of microeukaryotes and epi‐endophytes in the holobiome of seven species of large brown algae

18S rDNA gene metabarcoding of microeukaryotes and epi‐endophytes in the holobiome of seven species of large brown algae

An Assessment of the Influence of Host Species, Age, and Thallus Part on Kelp-Associated Diatoms

Epiphytic diatom community structure and richness is determined by macroalgal host and location in the South Shetland Islands (Antarctica)

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