An Endogenous Staphylococcus aureus CRISPR-Cas System Limits Phage Proliferation and Is Efficiently Excised from the Genome as Part of the SCC
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Mikkelsen, Kasper; Bowring, Janine; Ng, Yong Kai; Frisinger, Frida Svanberg; Maglegaard, Julie Kjærsgaard; Li, Qiuchun; Sieber, Raphael N.; Petersen, Andreas; Andersen, Paal Skytt; Rostøl, Jakob T.; Høyland‐Kroghsbo, Nina Molin; Ingmer, Hanne

doi:10.1128/spectrum.01277-23

Cited by 4 publications

(1 citation statement)

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“…Myoviruses have the largest genome of the three groups, carrying genes for a contractile tail as well as many accessory genes such as tRNAs and nucleases (O'Flaherty et al, 2004). S. aureus strains encode only a limited number of phage defense systems, the most prevalent being restriction-modification and abortive infection systems (Jurado et al, 2022), whereas Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems can only be found in 2.9% of S. aureus genomes (Mikkelsen et al, 2023). Furthermore, alteration of wall teichoic acid (WTA) polymers, can protect from phage infection.…”

Section: Introductionmentioning

confidence: 99%

Systematic classification of phage receptor-binding proteins predicts surface glycopolymer structure inStaphylococcuspathogens

Krusche,

Beck,

Lehmann

et al. 2024

Preprint

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SummaryWall teichoic acids (WTAs) are major surface polymers of staphylococcal pathogens and commensals, whose variable structure governs interaction with host receptors, immunoglobulins, and bacteriophages. The ribitol phosphate (RboP) WTA type contributes to virulence, for instance inStaphylococcus aureus, but we lack comprehensive knowledge of WTA types and cognate phages.We developed a computational pipeline to identify the receptor-binding proteins (RBPs) in 335 Staphylococcus phage genomes, yielding multiple distinct RBP clusters. Notably, many phages had two separate RBPs with in part different WTA preferences. RBP representatives differed in specificity for RboP WTA glycosylation types, recapitulating the specificity of the corresponding phage. Based on these results, we created a publicly available bioinformatic tool to predict phage host specificity based on RBP similarity.The RboP WTA specific Φ13-RBP also revealed that the presence of RboP WTA on non-aureus staphylococci is more common than previously thought. Our approach facilitates the characterization of opportunistic Staphylococcus pathogens according to WTA types, which has major implications for phage-mediated interspecies horizontal gene transfer and future phage therapies.

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Section: Introductionmentioning

confidence: 99%