An end-to-end workflow to study newly synthesized mRNA following rapid protein depletion inSaccharomyces cerevisiae
John B. Ridenour,
Rafal Donczew
Abstract:Accurate regulation of gene transcription by RNA polymerase II is essential for the growth and development of eukaryotic cells. Although significant progress has been made in understanding the mechanisms that regulate transcription, many questions remain unanswered. Defining the direct effects of transcriptional regulators is critically important to answering these questions. An effective approach for identifying the direct targets of transcriptional regulators is combining rapid protein depletion and quantifi… Show more
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