An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests

Serrano, Juan Alfonso; Huertas, Gloria; Maldonado-Jacobi, Andrés; Olmo, Alberto; Pérez, Pablo; Martín, María; Daza, Paula; Yufera, A.

doi:10.3390/s18072354

Cited by 13 publications

(30 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of electrical impedance spectroscopy has been studied for cell culture monitoring in different works for a wide variety of applications, such as the study of cell proliferation [ 1 , 27 , 28 , 29 ], cell toxicity [ 30 ], or cellular differentiation [ 31 ]. In [ 28 ], an oscillation-based circuit was proposed for the measurement of electrical impedance in cell cultures. Figure 6 a shows the equivalent model proposed in [ 28 ] for modeling the electrical behavior of the cell culture and instrumentation system.…”

Section: Discussionmentioning

confidence: 99%

“…In [28], an oscillation-based circuit was proposed for the measurement of electrical impedance in cell cultures. Figure 6a shows the equivalent model proposed in [28] for modeling the electrical behavior of the cell culture and instrumentation system. toxicity [30], or cellular differentiation [31].…”

Section: Range Of Porementioning

confidence: 99%

“… Electrical circuits used to model interfaces between impedance measurement electrodes and biological structures. ( a ) Electrical circuit used in [ 28 ], where Rgap models the resistance between the cell layer and the electrodes, Rs is the resistance between the cell layer and the reference electrode, R 1 and C 1 form the impedance of the area of the electrodes that is not covered by cells, and R 2 and C 2 model the impedance of the area of the electrodes covered by cells. ( b ) Electrical circuit used in [ 3 ], where R 0 models the overall electrolyte bulk behavior, R 1 and C 1 model the slower electrochemical phenomena (such as polarization due to mass diffusion), and R 2 and C 2 model the faster electrochemical phenomena (such as the polarization due to charge transfer).…”

Section: Figurementioning

confidence: 99%

See 2 more Smart Citations

Characterization and Monitoring of Titanium Bone Implants with Impedance Spectroscopy

Olmo

Hernández

Chicardi

et al. 2020

Sensors

Self Cite

View full text Add to dashboard Cite

Porous titanium is a metallic biomaterial with good properties for the clinical repair of cortical bone tissue, although the presence of pores can compromise its mechanical behavior and clinical use. It is therefore necessary to characterize the implant pore size and distribution in a suitable way. In this work, we explore the new use of electrical impedance spectroscopy for the characterization and monitoring of titanium bone implants. Electrical impedance spectroscopy has been used as a non-invasive route to characterize the volumetric porosity percentage (30%, 40%, 50% and 60%) and the range of pore size (100–200 and 355–500 mm) of porous titanium samples obtained with the space-holder technique. Impedance spectroscopy is proved to be an appropriate technique to characterize the level of porosity of the titanium samples and pore size, in an affordable and non-invasive way. The technique could also be used in smart implants to detect changes in the service life of the material, such as the appearance of fractures, the adhesion of osteoblasts and bacteria, or the formation of bone tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Range Of Porementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

See 1 more Smart Citation

Characterization and Monitoring of Titanium Bone Implants with Impedance Spectroscopy

Olmo

Hernández

Chicardi

et al. 2020

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…An initial electrical model of the cell culture can be drafted based on some previous works [18,23] (see Figure 7a). R gap models the resistance between the cell layer and the electrodes; R s models the resistance between the cell layer and the reference electrode; R 1 and C 1 form the impedance of the area of the electrodes that are not covered by cells; and R 2 and C 2 model the impedance of the area of the electrodes that is covered by cells.…”

Section: Electrical Modeling Of the Cellular Growth And Differentiationmentioning

confidence: 99%

“…Equation 1is reproduced following the equations of the oscillation-based test model described in [18,23]:…”

Section: Electrical Modeling Of the Cellular Growth And Differentiationmentioning

confidence: 99%

Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering

Olmo

Yuste

Serrano

et al. 2020

Sensors

Self Cite

View full text Add to dashboard Cite

In tissue engineering, of utmost importance is the control of tissue formation, in order to form tissue constructs of clinical relevance. In this work, we present the use of an impedance spectroscopy technique for the real-time measurement of the dielectric properties of skeletal myoblast cell cultures. The processes involved in the growth and differentiation of these cell cultures in skeletal muscle are studied. A circuit based on the oscillation-based test technique was used, avoiding the use of high-performance circuitry or external input signals. The effect of electrical pulse stimulation applied to cell cultures was also studied. The technique proved useful for monitoring in real-time the processes of cell growth and estimating the fill factor of muscular stem cells. Impedance spectroscopy was also useful to study the real-time monitoring of cell differentiation, obtaining different oscillation amplitude levels for differentiated and undifferentiated cell cultures. Finally, an electrical model was implemented to better understand the physical properties of the cell culture and control the tissue formation process.

show abstract

Conductive Polymer‐Based Bioelectronic Platforms toward Sustainable and Biointegrated Devices: A Journey from Skin to Brain across Human Body Interfaces

Bettucci

Matrone

Santoro

2021

Adv Materials Technologies

View full text Add to dashboard Cite

the human's body toward medical applications. [1] Coupling electronics with human tissues allows sensing, stimulating and possibly regulating biological functions. On these premises, a key paradigm of bioelectronics is to preserve the functionality of the biological environment upon coupling, in order to "observe biology through non-perturbing lenses". However, aiming to a seamless interface, the properties of common electronic components, based on metals or inorganic semiconductors, have major limitations to comply with the coupling requirements for cells, organs, and tissues. [2] In fact, inorganic materials might display mechanical rigidity (high Young's modulus ≈100 GPa), [3,4] surface degradation phenomena (oxidation) [5] and surface structure which increases interface capacitance. [6,7] Organic materials have so emerged combining mechanical flexibility, compatibility with large area and different surface functionalization processes, while keeping high electrical conductivity and reproducibility. [8,9] Particularly, these materials intrinsically display key properties such as tunable surface roughness, [10] surface chemical reactivity 11 , and Young's moduli ranging from 20 kPa to 3 GPa, [11,12] approaching the modulus of living tissue (10 kPa [13] ). However, the diversity of biological landscapes offered by the different human tissues, in terms of mechanical flexibility, interface functionalization, accessibility and biological activities defines sets of requirements so stringent that commonly for each tissue/organ coupling ad hoc devices and platforms have been developed. Hence, examining the three major human's body interfaces targeted by bioelectronics applications (skin, heart, and brain), this review focuses on the strategies that can be adopted by employing organic materials such as surface patterning, novel material synthesis and bio-functionalization in order to enhance tissue/organ-specific coupling performances. These aspects are investigated highlighting current and future main challenges and providing perspectives on the development of the next generation organic bioelectronics devices, thus envisioning systems that are: 1) able to adapt their interface in shape and size to comply with different curvilinear and hierarchical biological architectures and 2) combine sensing and stimulation to dynamically control biological functions for biomedical applications.

show abstract

An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests

Cited by 13 publications

References 17 publications

Characterization and Monitoring of Titanium Bone Implants with Impedance Spectroscopy

Characterization and Monitoring of Titanium Bone Implants with Impedance Spectroscopy

Electrical Modeling of the Growth and Differentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering

Conductive Polymer‐Based Bioelectronic Platforms toward Sustainable and Biointegrated Devices: A Journey from Skin to Brain across Human Body Interfaces

Contact Info

Product

Resources

About