An emphysema model in rats treated intratracheally with elastase

Yokoyama, Eiji; Nambu, Ziro; Uchiyama, Iwao; Kyono, Hiroko

doi:10.1016/s0013-9351(87)80199-8

Cited by 17 publications

(9 citation statements)

References 11 publications

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“…As in the present study, an increase in lung volume has been reported in living animals treated with intratracheal elastase (Kaplan et al 1973;Hayes et al 1975;Snider and Sherter 1977;Harkema 1984;Yokoyama et al 1987). However, when elastase was instilled intratracheally to excised lungs no increase in TLC was observed.…”

Section: Discussionsupporting

confidence: 83%

Dose dependency of intratracheal elastase-induced changes in pressure volume curve and morphometry in rat lungs.

Sato

Kato

Takahashi

et al. 1990

Tohoku J. Exp. Med.

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We studied the dose-dependency of the lung pressure-volume curve (n -37) and morphometry (n -30) with intratracheally administered elastase. Four weeks after elastase was instilled at dosages of 20 (EL-20), 40, 80, and 100 U/100 g wt., chord compliance between 50 and 70% of total lung capacity (TLC) (C5o-70) and that between 80 and 100% TLC (C8o-100) Were measured. After a significant initial reduction, the weight of the EL-80 group recovered to the control level, whereas the weight curve of the EL-100 significantly decreased below that of the EL-80. Lung volumes of all the elastase-treated groups were significantly larger than those of control. Air-filled lung volumes monotonously increased when the elastase dose was increased from 20 to 80 U/100 g wt. In contrast lung volume of the EL-100 was significantly lower than that of the EL-80. On the other hand, liquid-filled lung volumes monotonously increased from 20 to 100 U/100 g wt. Cao-ioo was significantly smaller in the EL-100 than in the EL-80. The mean linear intercept increased and alveolar surface area decreased monotonously over the dose range tested. We conclude that there is a critical dose of elastase below which lung volume is increased and above which the increase is suppressed when elastase is administered intratracheally to Wistar rats. pulmonary emphysema ; mean linear intercept ; lung compliance ; alveolar surface area

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Section: Discussionsupporting

confidence: 83%

Dose dependency of intratracheal elastase-induced changes in pressure volume curve and morphometry in rat lungs.

Sato

Kato

Takahashi

et al. 1990

Tohoku J. Exp. Med.

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“…Unlike Damon et al [18], we also observed increases in residual volume and total lung capacity. These changes in lung structure and pulmonary function are often seen in patients with COPD [ 10, 21-23] and are in agreement with a variety of animal studies utilizing lung elastase treatment to model emphysema [ 19,20,24]. Using the methods of Damon et al…”

Section: Effect Of Elastase On Lung Structure and Pulmonary Functionsupporting

confidence: 74%

Pulmonary Vagal Reflexes and Breathing Pattern are not Altered in Elastase-Induced Emphysema in Rats

Mansoor

Hyde

Schelegle

1997

Experimental Lung Research

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The role of nonmyelinated and myelinated vagal afferents in pulmonary reflexes and breathing pattern was examined in elastase-treated emphysemic rats. Fourteen to 17 days after intratracheal instillation of 1 IU/gm of porcine pancreatic elastase or 0.5 mL of saline, elastase-treated rats had a decreased alveolar surface area to volume of parenchyma (Sv) (42.44 +/- 1.7 vs. 31.51 +/- 1.1 mm2/mm3), increased quasistatic compliance (QSC) (1.05 +/- 0.06 vs. 1.25 +/- 0.09 mL/cm H2O), functional residual capacity (FRC) (4.31 +/- 0.10 vs. 5.88 +/- 0.37 mL), residual volume (RV) (3.02 +/- 0.14 vs. 4.27 +/- 0.31 mL), and total lung capacity (TLC) (14.04 +/- 0.28 vs. 15.58 +/- 0.54 mL). There were no changes in the strength of the pulmonary chemoreflex, the strength of the Hering-Breuer inflation reflex, or breathing pattern before or after vagal perineural capsaicin treatment (VPCT) or vagotomy. There were, however, significant negative correlations between Sv and TLC, FRC and RV, and a near significant (p < .09) negative correlation between Sv and QSC, but no significant correlations between Sv and indices of either the pulmonary chemoreflex or Hering-Breuer inflation reflex. The results indicate that pulmonary vagal nonmyelinated and myelinated reflex activity and breathing pattern are not affected by elastase-induced emphysema in rats.

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“…The increased mean ADC at EEV in the elastase group as compared with the saline group implies that the distal air space volume is larger in the elastase group. This increase is anticipated in the emphysema model, because the EEV increases because of decreased elastic recoil (18,19). It is difficult to comment on the lack of significant difference between the HB diffusion coefficients for both groups of animals because we used a pressure ventilator, which does not guarantee an equal tidal volume delivered to both sets of animals.…”

Section: Resultsmentioning

confidence: 97%

Detection of emphysema in rat lungs by using magnetic resonance measurements of ³ He diffusion

Chen

Hedlund

Möller

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

163

175

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An emphysema model in rats treated intratracheally with elastase

Cited by 17 publications

References 11 publications

Dose dependency of intratracheal elastase-induced changes in pressure volume curve and morphometry in rat lungs.

Dose dependency of intratracheal elastase-induced changes in pressure volume curve and morphometry in rat lungs.

Pulmonary Vagal Reflexes and Breathing Pattern are not Altered in Elastase-Induced Emphysema in Rats

Detection of emphysema in rat lungs by using magnetic resonance measurements of ³ He diffusion

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An emphysema model in rats treated intratracheally with elastase

Cited by 17 publications

References 11 publications

Dose dependency of intratracheal elastase-induced changes in pressure volume curve and morphometry in rat lungs.

Dose dependency of intratracheal elastase-induced changes in pressure volume curve and morphometry in rat lungs.

Pulmonary Vagal Reflexes and Breathing Pattern are not Altered in Elastase-Induced Emphysema in Rats

Detection of emphysema in rat lungs by using magnetic resonance measurements of 3 He diffusion

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Detection of emphysema in rat lungs by using magnetic resonance measurements of ³ He diffusion