An emerging Panton–Valentine leukocidin-positive CC5-meticillin-resistant Staphylococcus aureus-IVc clone recovered from hospital and community settings over a 17-year period from 12 countries investigated by whole-genome sequencing

Aloba, Bisola K.; Kinnevey, Peter M.; Monecke, Stefan; Brennan, Gráinne I.; O’Connell, Brian; Blomfeldt, Anita; McManus, Brenda A.; Schneider-Brachert, Wulf; Tkadlec, Jan; Ehricht, Ralf; Senok, Abiola; Bartels, Mette Damkjær; Coleman, David C.

doi:10.1016/j.jhin.2022.11.015

Cited by 3 publications

(4 citation statements)

References 42 publications

(63 reference statements)

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“…To place WA121 within the broader phylogenetic context with other more diverse S. aureus CC5 strains, a second SNP tree was constructed using representatives of CC5 subclades CC5-basal (N315 and CF-Marseille), CC5-I (DAR5810), CC5-IIA (USA100_OR-10) and CC5-IIB (USA100_CA126) [ 35 ]. We also included ST5 MRSA and methicillin-susceptible S. aureus (MSSA) strains previously isolated in WA (WAMRSA3, WAMRSA64, 1995-MRSA-WBG8381 [ 16 ] and 1995-MSSA-C36S [ 36 ]), a PVL-positive ST5-IVo isolate from the Northern Territory of Australia (TC-1024 [ 12 ]), a representative of an emerging Sri Lankan PVL-positive ST5 clade (strain 229895 [ 37 ]) and the poultry-associated ST5 isolates ED98 and B4-59C. ST5 isolates SR130 and SR231 [ 38 ] were also included since they were frequently identified as the top hits in blastn searches using regions of the 2010–15611 chromosome sequence as a query.…”

Section: Resultsmentioning

confidence: 99%

“…We also included ST5 MRSA and methicillin-susceptible S. aureus (MSSA) strains previously isolated in WA (WAMRSA3, WAMRSA64, 1995-MRSA-WBG8381 [16] and 1995-MSSA-C36S [36]), a PVL-positive ST5-IVo isolate from the Northern Territory of Australia (TC-1024 [12]), a representative of an emerging Sri Lankan PVL-positive ST5 clade (strain 229895 [37]) and the poultry-associated ST5 isolates ED98 and B4-59C. ST5 isolates SR130 and SR231 [38] were also included since they were frequently identified as the top hits in blastn searches using regions of the 2010–15611 chromosome sequence as a query.…”

Section: Resultsmentioning

confidence: 99%

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Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities

Ramsay,

Parahitiyawa,

Mowlaboccus

et al. 2023

Microbial Genomics

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In 2010 a single isolate of a trimethoprim-resistant multilocus sequence type 5, Panton–Valentine leucocidin-positive, community-associated methicillin-resistant Staphylococcus aureus (PVL-positive ST5 CA-MRSA), colloquially named WA121, was identified in northern Western Australia (WA). WA121 now accounts for ~14 % of all WA MRSA infections. To gain an understanding of the genetic composition and phylogenomic structure of WA121 isolates we sequenced the genomes of 155 WA121 isolates collected 2010–2021 and present a detailed genomic description. WA121 was revealed to be a single clonally expanding lineage clearly distinct from sequenced ST5 strains reported outside Australia. WA121 strains were typified by the presence of the distinct PVL phage φSa2wa-st5, the recently described methicillin resistance element SCCmecIVo carrying the trimethoprim resistance (dfrG) transposon Tn4791, the novel β-lactamase transposon Tn7702 and the epidermal cell differentiation inhibitor (EDIN-A) plasmid p2010-15611-2. We present evidence that SCCmecIVo together with Tn4791 has horizontally transferred to Staphylococcus argenteus and evidence of intragenomic movement of both Tn4791 and Tn7702. We experimentally demonstrate that p2010-15611-2 is capable of horizontal transfer by conjugative mobilization from one of several WA121 isolates also harbouring a pWBG749-like conjugative plasmid. In summary, WA121 is a distinct and clonally expanding Australian PVL-positive CA-MRSA lineage that is increasingly responsible for infections in indigenous communities in northern and western Australia. WA121 harbours a unique complement of mobile genetic elements and is capable of transferring antimicrobial resistance and virulence determinants to other staphylococci.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities

Ramsay,

Parahitiyawa,

Mowlaboccus

et al. 2023

Microbial Genomics

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“…MRSA infection remains a global health concern, proliferating and posing significant challenges ( Aloba et al, 2023 ). While current investigations primarily targeted tertiary and teaching hospitals ( Chen et al, 2022 ), scant data exist regarding the genetic traits and nosocomial outbreaks of MRSA in county-level hospitals.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, PVL can lead to tissue necrosis and leukocyte lysis, and it is considered a significant contributor to skin and soft tissue infections. MRSA is considered to be a major nosocomial infection pathogen, PVL-positive CC5 strains have reported to be associated with nosocomial outbreaks, demonstrating a trend of global dissemination ( Lindner et al, 2022 ; Aloba et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Genomic characterization and outbreak investigations of methicillin-resistant Staphylococcus aureus in a county-level hospital in China

Huang,

Zhu,

Yan

et al. 2024

Front. Microbiol.

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Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen contributing to healthcare-associated infections, which can result in multiple sites infections. The epidemiological characteristics of MRSA exhibit variability among distinct regions and healthcare facilities. The aim of this study was to investigate the molecular epidemiology and nosocomial outbreak characteristics of MRSA in a county-level hospital in China. A total of 130 non-repetitive MRSA strains were collected from December 2020 to November 2021. Whole-genome sequencing (WGS) was performed to identify antimicrobial resistance and virulence factors. Phylogenetic analysis was conducted to ascertain genetic diversity and phylogenetic relationships. Independent transmission scenarios were determined by the phylogeny derived from single nucleotide polymorphisms (SNPs) within the core genome. All the MRSA isolates were collected from the intensive care unit (30.00%, 39/130), the department of otorhinolaryngology (10.00%, 13/130) and the department of burn unit (9.23%, 12/130). The clinical samples mainly included phlegm (53.85%, 70/130), purulent fluid (24.62%, 32/130), and secretions (8.46%, 11/130). The resistance rates to erythromycin, clindamycin and ciprofloxacin were 75.38, 40.00, and 39.23%, respectively. All the isolates belonged to 11 clonal complexes (CCs), with the major prevalent types were CC5, CC59, and CC398, accounting for 30.00% (39/130), 29.23% (38/130), and 16.92% (22/130), respectively. Twenty sequence types (STs) were identified, and ST59 (25.38%, 33/130) was the dominant lineage, followed by ST5 (23.84%, 31/130) and ST398 (16.92%, 22/130). Three different SCCmec types were investigated, most of isolates were type IV (33.85%, 44/130), followed by type II (27.69%, 36/130) and type III (0.77%, 1/130). The common clonal structures included CC5-ST5-t2460-SCCmec IIa, CC59-ST59-t437-SCCmec IV and CC398-ST398-t034-SCCmec (−), with rates of 16.92% (22/130), 14.62% (19/130), and 13.84% (18/130), respectively. Only 12 panton-valentine leucocidin (PVL) positive strains were identified. Two independent clonal outbreaks were detected, one consisting of 22 PVL-negative strains belongs to CC5-ST5-t2460-SCCmec IIa and the other consisting of 8 PVL-negative strains belongs to CC5-ST5-t311-SCCmec IIa. Overall, our study indicated that the CC5 lineage emerged as the predominant epidemic clone of MRSA, responsible for nosocomial outbreaks and transmission within a county-level hospital in China, highlighting the necessity to strengthen infection control measures for MRSA in such healthcare facilities.

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Pathogenomic profile and clonal diversity of potential zoonotic MRSA-CC7-ST789-t091-SCCmecV from human skin and soft tissue infections

Akinduti,

Motayo,

Maged

et al. 2024

Sci Rep

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An emerging Panton–Valentine leukocidin-positive CC5-meticillin-resistant Staphylococcus aureus-IVc clone recovered from hospital and community settings over a 17-year period from 12 countries investigated by whole-genome sequencing

Cited by 3 publications

References 42 publications

Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities

Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities

Genomic characterization and outbreak investigations of methicillin-resistant Staphylococcus aureus in a county-level hospital in China

Pathogenomic profile and clonal diversity of potential zoonotic MRSA-CC7-ST789-t091-SCCmecV from human skin and soft tissue infections

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