An emergent science on the brink of irrelevance: a review of the past 8 years of DNA barcoding

Taylor, Helen R.; Harris, W. E.

doi:10.1111/j.1755-0998.2012.03119.x

Cited by 249 publications

(198 citation statements)

References 113 publications

Supporting

Mentioning

186

Contrasting

Unclassified

Order By: Relevance

“…Several Asiatic taxa are most closely related to B. cryptarum based on COI (Bertsch et al 2010) and the difficulty of defining the respective taxonomic units is an ongoing process (Bertsch et al 2014). Admittedly, although COI barcoding and its applicability for species recognition has been criticized (e.g., Will andRubinoff 2004, DeSalle et al 2005, Meyer and Paulay 2005, for a review see Taylor and Harris 2012), the results for the European B. lucorum-complex seem convincing. The interspecific genetic divergences of the species are considerably larger than the intraspecific divergences and these patterns are stable over wide geographic ranges of Europe.…”

Section: Nucleotide Sequence Data Improved Our Understandingmentioning

confidence: 96%

Recognition and identification of bumblebee species in the Bombus lucorum-complex (Hymenoptera, Apidae) – A review and outlook

Bossert¹

2015

DEZ

View full text Add to dashboard Cite

The recognition of cryptic species represents one of the major challenges in current taxonomy and affects our understanding of global diversity. In practice, the process from discovery to acceptance in the scientific community can take an extensive length of time. A prime example is the traditionally difficult taxonomy of the cryptic bumblebee species belonging to the Bombus lucorum-complex. The status of the three European species in the group -Bombus lucorum and the closely related Bombus cryptarum and Bombus magnus -has recently become widely accepted, primarily due to investigations of nucleotide sequences and marking pheromones. In contrast, doubts prevail concerning the validity of species identification based on morphology. As a consequence, our knowledge of the species is muddled in a mire of unreliable and confusing literature data from a large number of authors over the centuries. To clarify this issue, this paper provides a recapitulation of the historical literature and highlights the milestones in the process of species recognition. Further, the possibility of a morphologically based species identification is discussed in the context of new molecular data. Finally, this review outlines the current challenges and provides directions for future issues.

show abstract

Section: Nucleotide Sequence Data Improved Our Understandingmentioning

confidence: 96%

Recognition and identification of bumblebee species in the Bombus lucorum-complex (Hymenoptera, Apidae) – A review and outlook

Bossert¹

2015

DEZ

View full text Add to dashboard Cite

show abstract

“…Dengan ditemukannya teknik perbanyakan untai DNA melalui PCR (Polymerase Chain Reaction), maka ilmu biologi mengalami evolusi menjadi biologi molekuler, termasuk untuk identifikasi spesies. Taylor & Harrist (2012) menyebutkan bahwa identifikasi taksonomi, serta penentuan batasan spesies dapat dilakukan melalui barcoding gen tunggal atau lokus DNA. Lebih lanjut Hebert et al (2003) menjelaskan bahwa gen cytochrome oxydase (COI) dapat berfungsi sebagai barcode genetik untuk semua makhluk hidup.…”

Section: Pendahuluanunclassified

Dna Barcoding Ikan Hias Introduksi

Fahmi

Kusumah

Ardi

et al. 2017

Jurnal Riset Akuakultur

View full text Add to dashboard Cite

ABSTRAKIdentifikasi spesies menjadi tantangan dalam pengelolaan ikan hias introduksi baik untuk tujuan budidaya maupun konservasi. Penelitian ini bertujuan untuk melakukan identifikasi molekuler ikan hias introduksi yang beredar di pembudidaya dan pasar ikan hias Indonesia dengan menggunakan barcode DNA gen COI. Sampel ikan diperoleh dari pembudidaya dan importir ikan hias di kawasan Bandung dan Jakarta. Total DNA diekstraksi dari jaringan sirip ekor dengan menggunakan metode kolom.

show abstract

“…Such proposals have inevitably drawn criticism from taxonomists and others who are concerned at the empirical and theoretical flaws characterising 'DNA taxonomy' as it is currently practised (Taylor & Harris, 2012;Goldstein & DeSalle, 2011;Lipscomb et al, 2003;Seberg et al, 2003). DNA proponents argue that "DNA sequence information is digital and is not influenced by subjective assessments" (Tautz et al, 2003, p. 71).…”

Section: Dna Barcoding: At the Crossroadsmentioning

confidence: 99%

Optimising Capture Methods for the Evaluation of Parasitoid Wasp Diversity

Saunders¹

2018

Preprint

View full text Add to dashboard Cite

Parasitoid wasps are mega-diverse, ecologically dominant, but poorly studied components of global biodiversity. Despite their intensive application within pest management as biocontrol agents, little is known about native species. To understand their basic biology they must be collected in sampling programs. However, invertebrate surveys are increasingly subject to funding and time constraints that often preclude complete faunal inventories. In order to maximise the efficiency and reduce the cost of their collection, the application of optimal sampling techniques within a Rapid Biodiversity Assessment framework is proposed. Two sites in the Waitakere Ranges were sampled three times over the summer. An intensive sampling effort of 840 Malaise-trap-days over a three month period was used to determine the relationship between sampling effort and observed species richness. Rarefaction techniques and non-parametric estimators were used to predict true species richness and to evaluate the completeness of sampling. Results show that an intensive Malaise-trapping regime over the summer can capture two-thirds of parasitoid wasp species present. Sampling recommendations are provided to guide optimal usage of Malaise traps for both ecological studies and faunal inventories. Modern taxonomic methods are reviewed and a new species of parasitoid wasp is described, representing the first New Zealand species from the genus Lusius (Ichneumonidae: Ichneumoninae). Morphological measurements confirm the new species represents a significant range expansion for the genus. Greater collaboration between ecologists and taxonomists is encouraged, in order to make more efficient use of resources, data, and expertise unique to each discipline. This is the first study to investigate the relationship between sampling effort and parasitoid wasp diversity in New Zealand. It shows that very high sampling effort fails to catch all species present. Parasitoid wasps are known to be keystone species that show promise as indicators of environmental quality and as surrogates for the diversity of other taxa. The development of optimal sampling strategies will therefore provide an important foundation for their future study.

show abstract

An emergent science on the brink of irrelevance: a review of the past 8 years of DNA barcoding

Cited by 249 publications

References 113 publications

Recognition and identification of bumblebee species in the Bombus lucorum-complex (Hymenoptera, Apidae) – A review and outlook

Recognition and identification of bumblebee species in the Bombus lucorum-complex (Hymenoptera, Apidae) – A review and outlook

Dna Barcoding Ikan Hias Introduksi

Optimising Capture Methods for the Evaluation of Parasitoid Wasp Diversity

Contact Info

Product

Resources

About