An embryonic heart cell line is susceptible to dengue virus infection

Protocol to evaluate the antiviral effect of FDA-approved drugs against dengue virus in Huh7 cells and AG129 mice

“… Note: The plasmid containing the 151 bp corresponding to a region of the C protein of DENV was made by Angel-Ambrocio et al. 3 Note: To obtain the initial volume of the stock plasmid, use the following formula: Mass of one plasmid molecule. To calculate the mass of one plasmid molecule, the following formula is used: m=(n) (1.096 × 10 -21 grams/base pairs).…”

“… Note: The plasmid containing the 151 bp corresponding to a region of the C protein of DENV was made by Angel-Ambrocio et al. 3 Note: To obtain the initial volume of the stock plasmid, use the following formula: …”

“…Note: The plasmid containing the 151 bp corresponding to a region of the C protein of DENV was made by Angel-Ambrocio et al. 3 …”

The β 4 subunit of Ca v 1.2 channels is required for an optimal interferon response in cardiac muscle cells

“…For real-time PCR amplification, SYBR Fast universal (Kapa) was used in the Eco Illumina System apparatus. A standard curve was generated as described previously (23). The number of copies of the 151-bp RNA fragment encoding the capsid was estimated by interpolation in the standard curve.…”

“…These cells are also a good model in which to study viral infection in the heart. We previously showed that these cells are susceptible to infection by dengue virus (DENV) (23), a single-stranded positive RNA virus of the Flaviviridae family, the pathogenic agent that causes dengue fever, which can damage the heart, liver, lungs, and other organs and is potentially fatal (24)(25)(26). Previous work showed that Ca 2+ homeostasis is disturbed by viral proteins (27,28).…”