An ELISA immunoassay employing a conserved Leishmania hypothetical protein for the serodiagnosis of visceral and tegumentary leishmaniasis in dogs and humans

Carvalho, Ana Maria Ravena Severino; Costa, Lourena E.; Salles, Beatriz C.S.; Santos, Thaís T.O.; Ramos, Fernanda F.; Lima, Mariana P.; Chávez-Fumagalli, Miguel Á.; Silvestre, Bruna T.; Portela, Áquila S.B.; Roatt, Bruno Mendes; Silveira, Júlia A.G.; Gonçalves, Denise Utsch; Magalhães-Soares, Danielle F. de; Duarte, Mariana C.; Menezes‐Souza, Daniel; Coelho, Eduardo Antônio Ferraz

doi:10.1016/j.cellimm.2017.06.001

Cited by 20 publications

(14 citation statements)

References 63 publications

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“…Our research resulted in 315 manuscripts related to the serology of leishmaniasis by the combined application of descriptors in the databases described above. Only 14 studies remained ( Table 1 ) 20 , 22 , 24 , 25 , 27 , 32 - 40 after applying the eligibility criteria (duplicate texts, articles related to other topics, texts excluded due to review or quality method criteria). The studies evaluated the diagnostic accuracy of different specific and novel antigens.…”

Section: Resultsmentioning

confidence: 99%

Diagnostic accuracy of Enzyme-Linked Immunosorbent Assays to detect anti-Leishmania antibodies in patients with American Tegumentary Leishmaniasis: a systematic review

Zanetti

Sato

Longhi³

et al. 2019

Rev. Inst. Med. trop. S. Paulo

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American Tegumentary leishmaniasis (ATL) is an infectious disease caused by several species of Leishmania . Even though the direct detection of parasites has low sensitivity, it is still the gold standard for the laboratory diagnosis of ATL. Recent studies have shown promising results of Enzyme-Linked Immunosorbent Assays ( ELISAs) using recombinant antigens. The aim of this study is to compare the accuracy of ELISAs using novel antigens with the standard ELISA based on soluble antigens of Leishmania (SLA) to diagnose ATL. Studies that analyzed patients with ATL and studies that evaluated the diagnostic accuracy of ELISAs using novel antigens and SLA were included. The Fourteen studies from PubMed, Regional Portal of the Virtual Health Library (BVS), Brazilian Society of Dermatology, Virtual Health Library (IBECS), Literature in the Health Sciences in Latin America and the Caribbean (LILACS), Medical Literature Analysis and Retrieval System Online (Medline), Elsevier Embase, Cochrane Library, The National Institute for Health and Care Excellence (NICE), and Cumulative Index to Nursing and Allied Health Literature (CINAHL) were included. The novel ELISA antigens showed a high sensitivity (93.8%-100%) and specificity (82.5-100%), a better diagnostic performance than SLA-based ELISAs (1-97.4% and 57.5-100%, respectively). Only 10 studies analyzed cross-reactions in serum samples from patients with Chagas disease, and only two studies reported a percentage of cross-reactivity. In this systematic review, the novel ELISA antigens showed better sensitivity and specificity with respect to SLA-based ELISAs. However, a meta-analysis should be performed to confirm this finding.

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Section: Resultsmentioning

confidence: 99%

Diagnostic accuracy of Enzyme-Linked Immunosorbent Assays to detect anti-Leishmania antibodies in patients with American Tegumentary Leishmaniasis: a systematic review

Zanetti

Sato

Longhi³

et al. 2019

Rev. Inst. Med. trop. S. Paulo

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“…(12.5% - 1/8). Recently, Carvalho et al [15] proposed an ELISA using a L . infantum LiHypA recombinant antigen, which conferred 100% sensitivity and 99% specificity for CanL diagnosis, but it exhibited cross reactivity with B .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, many serologic assays can present false-positive results due to cross reactions with other species of Leishmania such as L . braziliensis [12], and with other infectious agents that are common in dogs, including Trypanosoma cruzi [13], Ehrlichia canis , Babesia canis [14, 15].…”

Section: Introductionmentioning

confidence: 99%

Leishmania infantum-derived lipophosphoglycan as an antigen in the accurate serodiagnosis of canine leishmaniasis

Portela

Soares

Passos³

et al. 2019

PLoS Negl Trop Dis

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Lipophosphoglycan (LPG) is the major surface glycoconjugate of Leishmania protozoan and has an important biological role in host-parasite interactions both in the midgut epithelium of the sand fly vector and in the vertebrate macrophages. Canine leishmaniasis (CanL) is a chronic infectious disease predominantly caused by Leishmania infantum. An early and accurate immunodiagnosis of the disease is crucial for veterinary clinical practice and for disease control. In this work, we evaluated L. infantum LPG as an antigen in an indirect enzyme-linked immunosorbent assay (ELISA) for CanL immunodiagnosis (LPG-ELISA) by testing serum samples from 97 naturally infected dogs with diverse clinical presentations ranging from subclinical infection to severe disease, as evaluated by veterinarian infectologists. Serum samples from healthy dogs from non-endemic areas (n = 68) and from dogs with other infectious diseases (n = 64) were used as controls for assay validation. The performance of the LPG-ELISA was compared with that of an ELISA using the soluble fraction of L. infantum total lysate antigen (TLA). LPG-ELISA presented a superior performance in comparison to TLA-ELISA, with 91.5% sensitivity, 98.5% specificity and 99.7% accuracy. A distinguishing feature of the LPG-ELISA compared to the TLA-ELISA was its higher ability to identify subclinical infection in clinically healthy dogs, in addition to the absence of cross-reactivity with other canine infectious diseases. Finally, LPG-ELISA was compared to TR DPP visceral canine leishmaniasis test, the immunochromatographic test recommended by the Brazilian Ministry of Agriculture. LPG-ELISA exhibited higher values of specificity (98.5% versus 93.1%) and sensitivity (91.5% versus 90.6%) compared to TR DPP. In conclusion, L. infantum-derived LPG was recognized by antibodies elicited during CanL in different infection stages and was shown to be a suitable antigen for specific clinical settings of veterinary diagnosis and for public health usage.

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“…In a recent study, a hypothetical protein from L. ( L. ) infantum (LiHyE) showed good results in terms of sensitivity in samples from patients with CL and VL, when compared to other antigens. This recombinant protein was considered to be a promising marker for differentiating healthy individuals from those infected with CL or VL [ 187 , 188 ].…”

Section: Immunological Methods For Diagnosis Of Leishmaniasismentioning

confidence: 99%

“…Both antigens showed good performance, however, rLb6H showed greater reactivity with L. ( L. ) amazonensis , followed by L. ( V. ) braziliensis and L. ( V. ) guyanensis . Other recombinant proteins, such as LiHypA, SMP-3, CcOx, HRF, rLiHyL, and others, were evaluated and presented good results, with some studies observing good sensitivity and specificity for both CL and VL [ 187 , 193 , 194 , 195 ]. In general, these recombinant proteins have higher sensitivity and specificity than the soluble Leishmania antigen extract, but they do not discriminate CL and VL infection.…”

Section: Immunological Methods For Diagnosis Of Leishmaniasismentioning

confidence: 99%

Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods

et al. 2020

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Leishmaniasis is a neglected tropical disease with two main clinical forms: cutaneous and visceral leishmaniasis. Diagnosis of leishmaniasis is still a challenge, concerning the detection and correct identification of the species of the parasite, mainly in endemic areas where the absence of appropriate resources is still a problem. Most accessible methods for diagnosis, particularly in these areas, do not include the identification of each one of more than 20 species responsible for the disease. Here, we summarize the main methods used for the detection and identification of leishmaniasis that can be performed by demonstration of the parasite in biological samples from the patient through microscopic examination, by in vitro culture or animal inoculation; by molecular methods through the detection of parasite DNA; or by immunological methods through the detection of parasite antigens that may be present in urine or through the detection of specific antibodies against the parasite. Potential new methods that can be applied for laboratory diagnosis of leishmaniasis are also discussed.

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An ELISA immunoassay employing a conserved Leishmania hypothetical protein for the serodiagnosis of visceral and tegumentary leishmaniasis in dogs and humans

Cited by 20 publications

References 63 publications

Diagnostic accuracy of Enzyme-Linked Immunosorbent Assays to detect anti-Leishmania antibodies in patients with American Tegumentary Leishmaniasis: a systematic review

Diagnostic accuracy of Enzyme-Linked Immunosorbent Assays to detect anti-Leishmania antibodies in patients with American Tegumentary Leishmaniasis: a systematic review

Leishmania infantum-derived lipophosphoglycan as an antigen in the accurate serodiagnosis of canine leishmaniasis

Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods

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