An ELISA-based method for measurement of food-specific IgE antibody in mouse serum: an alternative to the passive cutaneous anaphylaxis assay

Birmingham, Neil P.; Payankaulam, Sandhya; Thanesvorakul, Sirinart; Stefura, Bill P.; HayGlass, Kent T.; Gangur, Venu

doi:10.1016/s0022-1759(03)00008-5

Cited by 38 publications

(35 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have previously described optimization of enzyme-linked immunosorbent assay (ELISA) for food-specific IgG1, IgG2a and IgE antibody analyses [17, 18]. The ELISA procedure used in this study was essentially as described by us before using pooled plasma samples [17, 18].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

An Adjuvant-Free Mouse Model of Tree Nut Allergy Using Hazelnut as a Model Tree Nut

Birmingham¹,

Parvataneni²,

Hassan³

et al. 2007

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Background: Tree nut allergy, a major group of food allergy, is often linked to fatal or near-fatal systemic anaphylaxis. Currently, an adjuvant-free mouse model to study tree nut hypersensitivity is unavailable. Here we tested the hypothesis that transdermal exposure to hazelnut, a model tree nut, without the use of an adjuvant is sufficient to sensitize mice for immediate hypersensitivity reaction to oral hazelnut challenge. Methods: BALB/c mice were repeatedly exposed to hazelnut protein via the transdermal route and systemic allergic and anaphylactic responses were studied. Results: Transdermal exposure to hazelnut protein elicited robust systemic IgE response in a dose-dependent manner with immunological memory. Oral challenge of transdermally sensitized mice with hazelnut protein resulted in immediate (30 min after the challenge) clinical signs of systemic anaphylaxis as measured by significant clinical scores and drop in rectal temperature. Clinical hypersensitivity reaction was associated with severe pathological changes in the small intestine. Hazelnut-allergic but not control mice exhibited in vivo activation of GATA-3 and hazelnut-driven recall IL-4, IL-5 and IL-13 response by splenocytes, thus elucidating the underlying mechanism of hazelnut allergy development in this model. Conclusions: These data suggest that (1) transdermal exposure to hazelnut protein is sufficient to activate the key immune pathways necessary for sensitizing mice for clinical immediate hypersensitivity reactions and (2) this mouse model may be useful for further basic and applied studies on tree nut allergy, especially because it does not depend on an adjuvant for eliciting immediate hypersensitivity reactions to nut protein.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The ELISA procedure used in this study was essentially as described by us before using pooled plasma samples [17, 18]. Total plasma IgE levels were measured using paired antibodies and isotype standard purchased from BD Pharmingen.…”

Section: Methodsmentioning

confidence: 99%

An Adjuvant-Free Mouse Model of Tree Nut Allergy Using Hazelnut as a Model Tree Nut

Birmingham¹,

Parvataneni²,

Hassan³

et al. 2007

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…We have previously described optimization of enzyme-linked immunosorbent assay (ELISA) for food-specific IgG1, IgG2a and IgE antibody analyses [11, 12]. The ELISA procedure used in this study was essentially as described by us before using pooled plasma samples [11, 12].…”

Section: Methodsmentioning

confidence: 99%

“…The ELISA procedure used in this study was essentially as described by us before using pooled plasma samples [11, 12]. …”

Section: Methodsmentioning

confidence: 99%

Hazelnut Allergy: Evidence that Hazelnut Can Directly Elicit Specific IgE Antibody Response via Activating Type 2 Cytokines in Mice

Birmingham¹,

Gangur

Samineni³

et al. 2005

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Background: Hazelnut is one of the major tree nuts that causes potentially fatal food allergy, with underlying mechanisms that are unclear at present. One suggestion is that hazelnut allergy results from immune crossreactivity of IgE antibodies produced against certain aeroallergens. We tested the hypothesis that hazelnut is intrinsically capable of eliciting an allergic response using a mouse model. Methods: Groups of mice were injected intraperitoneally with hazelnut/filbert protein extract with or without alum as an adjuvant, and hazelnut-specific antibody (IgE, IgG1) responses were examined using optimized enzyme-linked immunosorbent assay. Hazelnut-specific type 2 and type 1 cytokine responses were evaluated by ex vivo antigen-mediated activation of spleen cells. Results: Hazelnut elicited robust IgE and IgG1 antibody responses. Timecourse and dose-response analyses further provided evidence for memory type 2-dependent antibody responses to hazelnuts. Hazelnut-specific IgE response in two strains of mice with different MHC haplotypes and IgE response to hazelnut without the use of alum adjuvant asserted that hazelnut is intrinsically an allergenic food. The type 2 cytokine analyses revealed that hazelnut sensitization results from activation of IL-4 and IL-5, thus providing a mechanistic basis for hazelnut-specific IgE response. Conclusion: Our data argue that hazelnut – a widely consumed food – is intrinsically an allergenic food capable of directly eliciting hazelnut-binding specific IgE antibodies viaactivation of type 2 cytokines in mice.

show abstract

“…Food allergies could be assessed by some immunoanalytical methods, such as ELISA tests, which determine both IgE-and IgG-mediated food allergies and often help in the identification of allergies [91][92][93][94][95].…”

Section: Diet and Nutritionmentioning

confidence: 99%

Candidiasis: Predisposing Factors, Prevention, Diagnosis and Alternative Treatment

et al. 2014

View full text Add to dashboard Cite

SUMMARYCandidiasis is the most common opportunistic yeast infection. Candida species and other microorganisms are involved in this complicated fungal infection, but Candida albicans continues to be the most prevalent. In the past two decades, it has been observed an abnormal overgrowth in the gastrointestinal, urinary and respiratory tracts, not only in immunocompromised patients, but also related to nosocomial infections and even in healthy individuals. There is a widely variety of causal factors that contribute to yeast infection which means that candidiasis is a good example of a multifactorial syndrome. Due to rapid increase in the incidence of these infections, this is the subject of numerous studies. Recently, the focus of attention is the treatment and, above all, the prevention of those complications. The diagnosis of candidiasis could become quite complicated. Prevention is the most effective "treatment", much more than eradication of the yeast with antifungal agents. There are several aspects to consider in the daily routine that can provide a strength protection. However, a therapeutic approach is necessary when the infection is established and, therefore, other alternatives should be explored. This review provides an overview on predisposition factors, prevention and diagnosis of candidiasis, highlighting alternative approaches for candidiasis treatment.

show abstract

An ELISA-based method for measurement of food-specific IgE antibody in mouse serum: an alternative to the passive cutaneous anaphylaxis assay

Cited by 38 publications

References 25 publications

An Adjuvant-Free Mouse Model of Tree Nut Allergy Using Hazelnut as a Model Tree Nut

An Adjuvant-Free Mouse Model of Tree Nut Allergy Using Hazelnut as a Model Tree Nut

Hazelnut Allergy: Evidence that Hazelnut Can Directly Elicit Specific IgE Antibody Response via Activating Type 2 Cytokines in Mice

Candidiasis: Predisposing Factors, Prevention, Diagnosis and Alternative Treatment

Contact Info

Product

Resources

About