An ELISA assay for the rapid diagnosis of acute bacterial meningitis

Salih, M. A. M.; Ahmed, A. A.; Ahmed, H. S.; Olcén, Per

doi:10.1080/02724936.1995.11747783

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…18 CSF samples collected on admission were subjected to laboratory analysis including cell count, glucose level, Gram stain using direct microscopy, bacteriological culture and ELISA (Meningitis EIA test, Karo-Bio Diagnostics, Sweden). 19 Cultures on blood chocolate agar and MacConkey agar were incubated at 37 o C aerobically and read after 48 hours. Chocolate agar plates were incubated in candle jars.…”

Section: Methodsmentioning

confidence: 99%

Epidemiological, Clinical and Prognostic Profile of Acute Bacterial Meningitis among Children in Alexandria, Egypt

Farag¹,

Abdel-Fattah²,

Youssri³

2005

Indian J Med Microbiol

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Purpose: To address the epidemiological characteristics and clinical indices that may predict the prognostic profile of meningitis among children. Methods: Children admitted to Alexandria fever hospital with clinical diagnosis of meningitis/ meningoencephalitis during the period 2002-2003 were recruited for the study. They were subjected to clinical examination as well as CSF bacteriological and serological investigations Results: Three hundred and ten patients (195 males and 115 females) were included. About 65.2% of them were infected with acute bacterial meningitis (ABM) and 34.8% were infected with aseptic meningitis. In this study, ABM was caused by Haemophilus influenzae (21%), Streptococcus pneumoniae (13.9%), Neisseria meningitidis (14.2%) and other undetermined bacteria (16.1%). ABM showed significant association with age group 1-9 years (66.3%), low socio-economic class (96%), working mother (83.2%), more than two smokers in the family (62.9%) and cold seasons(fall 35.1% and winter 48.5%). Aseptic meningitis showed significant association with age group 3-15 months (100%) and previous immunization(81.5%). The overall case fatality rate was 10.3%; 13.9% for ABM and 3.4% for aseptic meningitis. 7.1% of all survivors developed epileptic attacks. Predictors for death or epilepsy events were high WHO meningitis score (>9), decreased CSF glucose level (<10 mg/dL), more smokers in the family, generalised seizures, infancy (<1year of age) and working mothers. Conclusion: This study highlights the importance of several predictors of the outcome of meningitis in children. It is concluded that quick and simple scoring scales, such as the WHO scale, are not only applicable but valuable prognostic tools for meningitis in children.

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Section: Methodsmentioning

confidence: 99%

Epidemiological, Clinical and Prognostic Profile of Acute Bacterial Meningitis among Children in Alexandria, Egypt

Farag¹,

Abdel-Fattah²,

Youssri³

2005

Indian J Med Microbiol

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“…Opn is secreted in non-phosphorylated (Kubota et al, 1989;Chambers et al, 1992;Chang & Prince, 1993;Barber et al, 1999) and phosphorylated (Salih et al, 1995(Salih et al, , 1997Sorensen et al, 1994) forms, which contains up to 28 phosphate residues and are differentially induced by cytokines. Phosphorylation appears to be functionally important in determining whether Opn binds to cell surface receptors or the extracellular matrix (Nemir et al, 1989;Ek-Rylander et al, 1994).…”

Section: Osteopontinmentioning

confidence: 99%

Osteopontin: a bridge between bone and blood

2006

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SummaryThe production of mature blood cells within the bone marrow (BM) is attributed to a pool of haemopoietic stem cells (HSC). It is now evident that HSC reside preferentially at the endosteal region within the BM where bone-lining osteoblasts are a key cellular component of the HSC niche that directly regulates HSC fate. Osteoblasts synthesise proteins that stimulate and inhibit HSC proliferation. In addition to angiopoietin 1 (Ang-1), osteoblasts synthesise and express the highly acidic glycoprotein, osteopontin (Opn), which, like Ang-1, acts as a potent constraining factor on HSC proliferation. Overexpression of Opn is a feature of haemopoietic malignancies, such as multiple myeloma and chronic myeloid leukaemia, although its exact role in the aetiology and progression of these diseases remains unclear. Through osteoblasts and their cell surface and expressed proteins including Opn, bone is able to regulate the tissue that resides within it. In doing so, Opn can be considered a bridge between bone and blood.

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Neuroinfektionen und vernachlässigte Tropenkrankheiten (NTD)

Babikir,

Singh

2023

Neuroinflammation Und Neuroinfektion in Der Neuropädiatrie

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An ELISA assay for the rapid diagnosis of acute bacterial meningitis

Cited by 6 publications

References 23 publications

Epidemiological, Clinical and Prognostic Profile of Acute Bacterial Meningitis among Children in Alexandria, Egypt

Epidemiological, Clinical and Prognostic Profile of Acute Bacterial Meningitis among Children in Alexandria, Egypt

Osteopontin: a bridge between bone and blood

Neuroinfektionen und vernachlässigte Tropenkrankheiten (NTD)

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