An elegant technology for ultrasensitive impedimetric and voltammetric determination of cholestanol based on a novel molecularly imprinted electrochemical sensor

Abstract

In this work, a novel molecularly imprinted electrochemical sensor (MIES) has been fabricated based on electropolymerization of a molecularly imprinted polymer (MIP) onto a glassy carbon electrode (GCE) modified with gold-palladium alloy nanoparticles (AuPd NPs)/polydopamine film (PDA)/multiwalled carbon nanotubeschitosan-ionic liquid (MWCNTs-CS-IL) for voltammetric and impedimetric determination of cholestanol (CHO).Modifications applied to the bare GCE formed an excellent biocompatible composite film which was able to selectively detect CHO molecules. Modifications applied to the bare GCE were characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (SEM). Under optimal experimental conditions, the sensor was able to detect CHO in the range of 0.1-60 pM and 1-50 pM by EIS and DPV, respectively. Moreover, the sensor showed high sensitivity, selectivity, repeatability, reproducibility, low interference and good stability towards CHO determination. Our records confirmed that the sensor was successfully able to the analysis real samples for determination of CHO.

Molecularly Imprinted Polymers for Chemical Sensing: A Tutorial Review

“…To increase conductivity and obtain a better signal, graphite powder [33] and nanoparticles [71][72][73] have been included in these sensors. Morphine [21,56], ephedrine [57], paracetamol [58], dopamine [59,71], homovanillic acid [74], atrazine [60], creatinine [61], cholestanol [64], triacetone triperoxide [24], 2,4,6-trinitrotoluene, and 1,3,5-trinitroperhydro-1,3,5-triazine explosives [63] (see Table 1 for the characteristics of these sensors) are representative analytes. The analytic results obtained for the detection of creatinine using a MIP-based sensor showed comparable precision and accuracy with results obtained by HPLC [61].…”

Functional Ionic Liquids Decorated Carbon Hybrid Nanomaterials for the Electrochemical Biosensors

“…Consequently, they observed that the effective imprinting site could be greatly affecting the sensitivity of the sensor. Moreover, the specificity of the sensor is poor, as the concentration of 1.0 µM vitamin D3, 0.1 µM progesterone, and 10.0 µM cholesterol can significantly affect the result [ 141 ]. In another study, Boobphahom et al detected the creatinine in serum sample on a non-enzymatic, electrochemical technique integrated paper-based microfluidic device.…”

Remedial Effects and Molecular Docking Studies of Nevadensin: ‎Antiglucosidase, Anti-cholinesterase, and Anti-Human Oral Squamous ‎Cell Carcinoma Potentials

“…The medium was added dropwise to the vial and then its contents were taken out and centrifuged with the medium in 15 cc sterile test tubes. After centrifugation, the supernatant was removed and the cells were suspended again in the medium and transferred to a pre-prepared flask containing the medium and FBS and incubated (Hemmati et al, 2020;Jalalvand et al, 2020;Liu et al, 2020;Mahdavi et al, 2019;Nematpour et al, 2020;Zangeneh et al, 2019).…”

“…In another column, it was considered to contain culture medium and healthy cells and in other columns, it was considered to contain culture medium and cell line cells. One of these columns, which contained culture medium and cells and did not contain Nevadensin, was considered as a control (Hemmati et al, 2020;Jalalvand et al, 2020;Liu et al, 2020;Mahdavi et al, 2019;Nematpour et al, 2020;Zangeneh et al, 2019).…”

“…Wells containing cells without Nevadensin were considered as control and the optical density of wells without cells and only culture medium were considered as blank. The percentage of cell viability was calculated using the following formula (Hemmati et al, 2020;Jalalvand et al, 2020;Liu et al, 2020;Mahdavi et al, 2019;Nematpour et al, 2020;Taslimi et al, 2020;Türkan et al, 2020;Zangeneh et al, 2019):…”